Abstract
Human pharmaceuticals, such as nonsteroidal anti-inflammatory drugs (NSAIDs), are an emerging threat to marine organisms. NSAIDs act through inhibition of cyclooxygenase (COX) conversion of arachidonic acid into prostaglandins. One experiment was carried out whereby marine mussels were exposed for 72 h to 1 and 100 μg/L diclofenac (DCF). A specific and sensitive method using liquid chromatography high-resolution tandem mass spectrometry was developed to quantify DCF in mussel tissues. The developed method could also clearly identify and quantify COX products, i.e., prostaglandin levels, and be used to assess their modulation following DCF exposure. Prostaglandin-D2 (PGD2) was always found below the detection limit (20 μg/kg dry weight (dw)). Basal prostaglandin-E2 (PGE2) concentrations ranged from below the detection limit to 202 μg/kg dw. Exposure of 100 μg/L resulted in a significant reduction in PGE2 levels, whereas a downward trend was observed at 1 μg/L exposure. No difference was observed for prostaglandin-F2α (PGF2α) levels between controls and exposed organisms.
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Acknowledgments
Funding support was obtained from the The French National Research Program for Environmental and Occupational Health of Anses (AMeCE 2015/1/091) and the Agence Nationale de la Recherche (IMAP ANR-16-CE34-0006-01). This research benefited from the support of the Chair Veolia Environnement-HydroSciences: Risk analysis relating to emerging contaminants in water bodies. The doctoral fellowship of Bénilde Bonnefille is financially supported by a grant from the Université de Montpellier and Sanofi.
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Courant, F., Arpin-Pont, L., Bonnefille, B. et al. Exposure of marine mussels to diclofenac: modulation of prostaglandin biosynthesis. Environ Sci Pollut Res 25, 6087–6094 (2018). https://doi.org/10.1007/s11356-017-9228-6
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DOI: https://doi.org/10.1007/s11356-017-9228-6