Impact of metal stress on the production of secondary metabolites in Pteris vittata L. and associated rhizosphere bacterial communities

  • Hoang Nam Pham
  • Serge Michalet
  • Josselin Bodillis
  • Tien Dat Nguyen
  • Thi Kieu Oanh Nguyen
  • Thi Phuong Quynh Le
  • Mohamed Haddad
  • Sylvie Nazaret
  • Marie-Geneviève Dijoux-Franca
Research Article

DOI: 10.1007/s11356-017-9167-2

Cite this article as:
Pham, H.N., Michalet, S., Bodillis, J. et al. Environ Sci Pollut Res (2017). doi:10.1007/s11356-017-9167-2
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Abstract

Plants adapt to metal stress by modifying their metabolism including the production of secondary metabolites in plant tissues. Such changes may impact the diversity and functions of plant associated microbial communities. Our study aimed to evaluate the influence of metals on the secondary metabolism of plants and the indirect impact on rhizosphere bacterial communities. We then compared the secondary metabolites of the hyperaccumulator Pteris vittata L. collected from a contaminated mining site to a non-contaminated site in Vietnam and identified the discriminant metabolites. Our data showed a significant increase in chlorogenic acid derivatives and A-type procyanidin in plant roots at the contaminated site. We hypothesized that the intensive production of these compounds could be part of the antioxidant defense mechanism in response to metals. In parallel, the structure and diversity of bulk soil and rhizosphere communities was studied using high-throughput sequencing. The results showed strong differences in bacterial composition, characterized by the dominance of Proteobacteria and Nitrospira in the contaminated bulk soil, and the enrichment of some potential human pathogens, i.e., Acinetobacter, Mycobacterium, and Cupriavidus in P. vittata’s rhizosphere at the mining site. Overall, metal pollution modified the production of P. vittata secondary metabolites and altered the diversity and structure of bacterial communities. Further investigations are needed to understand whether the plant recruits specific bacteria to adapt to metal stress.

Keywords

Bacterial communities Metal stress Pteris vittata Rhizosphere Secondary metabolites 

Abbreviations

UHPLC-DAD-ESI/QTOF-MS

Ultrahigh-performance liquid chromatography with diode array detection coupled to electrospray ionization and quadrupole time-of-flight mass spectrometry

UV

Ultraviolet

ESI

Electrospray ionization

MSMS

Tandem mass spectrometry

ESI/MS2

Electrospray ionization tandem mass spectrometry

HRMS

High-resolution mass spectrometry

1H-NMR

Proton nuclear magnetic resonance

RT

Retention time

SPE

Solid phase extraction

PVP

Soil under P. vittata polluted

BSP

Bulk soil polluted

DLP

Soil under Dicranopteris linearis polluted

PVC

Soil under P. vittata control

HSD

Honest significant difference

PCA

Principal component analysis

ANOVA

Analysis of variance

CFU

Colony forming unit

QPCR

Quantitative real-time polymerase chain reaction

OTU

Operational taxonomic unit

DGGE

Denaturing gradient gel electrophoresis

DPPH

2,2-diphenyl-1-picrylhydrazyl

Supplementary material

11356_2017_9167_MOESM1_ESM.docx (157 kb)
Fig. S1Typical UHPLC/DAD chromatogram at 280 nm of P. vittata root extracts. PVP: From plants grown on contaminated soil (Ha Thuong); PVC: From plants grown on non-polluted soil (USTH) (DOCX 157 kb).
11356_2017_9167_MOESM2_ESM.docx (183 kb)
Fig. S2Typical UHPLC/DAD chromatogram at 280 nm of P. vittata stems extracts. PVP: From plants grown on contaminated soil (Ha Thuong); PVC: From plants grown on non-polluted soil (USTH) (DOCX 182 kb).
11356_2017_9167_MOESM3_ESM.docx (186 kb)
Fig. S3Typical UHPLC/DAD chromatogram at 280 nm of P. vittata leaves extracts. PVP: From plants grown on contaminated soil (Ha Thuong); PVC: From plants grown on non-polluted soil (USTH) (DOCX 185 kb).
11356_2017_9167_MOESM4_ESM.docx (12 kb)
Table S1(DOCX 11 kb).

Funding information

Funder NameGrant NumberFunding Note
Ministry of Education and Training of Vietnam
    University of Science and technology of Hanoi

      Copyright information

      © Springer-Verlag Berlin Heidelberg 2017

      Authors and Affiliations

      • Hoang Nam Pham
        • 1
        • 2
      • Serge Michalet
        • 1
      • Josselin Bodillis
        • 1
      • Tien Dat Nguyen
        • 3
      • Thi Kieu Oanh Nguyen
        • 2
      • Thi Phuong Quynh Le
        • 4
      • Mohamed Haddad
        • 5
      • Sylvie Nazaret
        • 1
      • Marie-Geneviève Dijoux-Franca
        • 1
      1. 1.UMR 5557, Ecologie Microbienne, CNRS, INRA, VetagroSup, UCBLUniversité de LyonVilleurbanneFrance
      2. 2.University of Science and Technology of Hanoi, Vietnam Academy of Science and TechnologyHanoiVietnam
      3. 3.Institute of Marine BiochemistryVietnam Academy of Science and TechnologyHanoiVietnam
      4. 4.Institute of Natural Products ChemistryVietnam Academy of Science and TechnologyHanoiVietnam
      5. 5.UMR 152 Pharma-DEVUniversité de Toulouse, IRD, UPSToulouseFrance

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