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Assessing PAHs pollution in Shandong coastal area (China) by combination of chemical analysis and responses of reproductive toxicity in crab Portunus trituberculatus

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Abstract

The concentrations of PAHs in seawater and sediments were measured at three selected sites (S1, S2, and S3) along the coastal area of Shandong (China) in April, May, and June, 2015, which ranged from 29.72 to 123.88 ng/L and 82.62 to 232.63 ng/g, respectively. Meanwhile, the reproductive toxicity responses in crab Portunus trituberculatus were also evaluated to assess the pollution of PAHs during the sampling period. Chemical analysis showed that S3 was the most PAH-contaminated area while S1 was the least, and the biochemical parameters concerned with reproduction were efficiently responded to the three sites, especially in S3 (p < 0.05). Moreover, the principal component analyses (PCA) showed that parameters for DNA alkaline unwinding, protein carbonyl content, and lipid peroxidation levels in two genders, 17β-estradiol in female, testosterone and TESK2 gene expression in male crabs, were closely correlated with the concentrations of PAHs (2 + 3 rings, 4 rings, and 5 + 6 rings), which were considered to be good candidate indicators to assess the environmental pollutions resulting from PAHs in the coastal area of Shandong, China.

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Acknowledgments

This work was supported by the State Oceanic Administration specific public project of China (201405010). We thank the staff of the Laboratory of Physiology for the help with sampling and taking care of the crabs.

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Authors and Affiliations

  1. The Key Laboratory of Mariculture, Ministry of Education, Ocean University of China, Qingdao, 266003, People’s Republic of China

    Luqing Pan, Ruiyi Xu, Jianmin Wen & Ruiming Guo

  2. Fisheries College, Ocean University of China, Yushan Road 5, Qingdao, 266003, People’s Republic of China

    Luqing Pan, Ruiyi Xu, Jianmin Wen & Ruiming Guo

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  1. Luqing Pan
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Correspondence to Luqing Pan.

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Pan, L., Xu, R., Wen, J. et al. Assessing PAHs pollution in Shandong coastal area (China) by combination of chemical analysis and responses of reproductive toxicity in crab Portunus trituberculatus . Environ Sci Pollut Res 24, 14291–14303 (2017). https://doi.org/10.1007/s11356-017-8993-6

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