Formetanate toxicity and changes in antioxidant enzyme system of Apis mellifera larvae
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Substantial percentage of world food production depends on pollinating service of honeybees that directly depends on their health status. Among other factors, the success of bee colonies depends on health of developed larvae. The crucial phase of larval development is the first 6 days after hatching when a worker larva grows exponentially and larvae are potentially exposed to xenobiotics via diet. In the present study, we determined the lethal concentration LC50 (72 h) following single dietary exposure of honeybee larvae to formetanate under laboratory conditions, being also the first report available in scientific literature. Activities of antioxidant enzymes, superoxide dismutase (SOD), catalase (CAT) and glutathione-S-transferase (GST) were also measured in the homogenates of in vitro reared honeybee larvae after single formetanate exposure. Decreased specific activity of SOD and increased activities of CAT and GST suggest the induction of oxidative stress. Higher levels of thiobarbituric reactive species in all samples supported this fact. Comparing determined larval toxicity (LC50 of 206.01 mg a.i./kg diet) with adult toxicity data, we can suppose that the larvae may be less sensitive to formetanate than the adult bees.
KeywordsHoney bee larvae Enzymes Carbamate Dietary exposure
Funding was provided by the Slovak Grant Agency VEGA (grant No. 1/0858/16 and No. 1/0176/16) and by the National Reference Laboratory for Pesticides of University of Veterinary Medicine and Pharmacy in Košice, Slovakia. The authors would like to thank Mgr. Tomáš Plichta for his help during the larvae rearing.
- European Food Safety Authority . 2006. Conclusion regarding the peer review of the pesticide risk assessment of the active substance formetanate. The EFSA Journal, [Internet]. [cited2016 Oct 10] 4(6):RN-69, 1–78 Available from: doi: 10.2903/j.efsa.2006.69r
- Fast PG (1964) Insect lipids: a review. Mem Entomol Soc Can 37:1–50Google Scholar
- Gómez-Ramos MM, García-Valcárcel AI, Tadeo JL, Fernández-Alba AR, Hernando MD (2016) Screening of environmental contaminants in honey bee wax comb using gas chromatography–high-resolution time-of-flight mass spectrometry. Environ Sci Pollut Res 23:4609. doi: 10.1007/s11356-015-5667-0 CrossRefGoogle Scholar
- Mittapalli O, Neal JJ, Shukle RH. 2007. Tissue and life stage specificity of glutathione S-transferase expression in the Hessian fly, Mayetiola destructor: implications for resistance to host allelochemicals. J Insect Sci [Internet]. [cited 2016 March 2]; 7: 1–13. Available from: http://jinsectscience.oxfordjournals.org/content/jis/7/1/20.full.pdf 10.1673/031.007.2001
- Nielsen SA, Brødsgaard CJ, Hansen H. 2000. Effects on detoxification enzymes in different life stages of honeybees (Apis mellifera L., Hymenoptera: Apidae) treated with a synthetic pyrethroid (flumethrin). Altern Lab Anim 28(3):437–443 [Abstract]Google Scholar
- Niemann RA (1993) Determination of formetanate hydrochloride in selected fruits by coupled-column cation exchange liquid chromatography. J AOAC Int 76:1362–1368Google Scholar
- OECD. 1998. Guideline for the Testing of Chemicals No. 213: Honey bee, Acute Oral Toxicity Test, Section 2; Effects on Biotic Systems, OECD, Paris 10.1787/9789264070165-en
- OECD. 2013. Guideline for the Testing of Chemicals No. 237: Honey Bees (Apis mellifera) Larval Toxicity Test, Single Exposure, Section 2: Effects on Biotic Systems 10.1787/9789264203723-en
- OECD. 2016. Guidance Document on Honey Bee Larval Toxicity Test following Repeated Exposure No. 239, [Internet]. [cited 2016 Oct 10] Available at: http://www.oecd.org/officialdocuments/publicdisplaydocumentpdf/?cote=ENV/JM/MONO(2016)34&docLanguage=En.
- Park YC, Lee S, Cho MH. 2014. The Simplest Flowchart Stating the Mechanisms for Organic Xenobiotics-induced Toxicity: Can it possibly be accepted as a “Central Dogma” for Toxic Mechanisms? Toxicol Res [Internet]. [cited 2016 May 12]; 30(3): 179–184. Available from: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4206744/pdf/toxicr-30-179.pdf doi: 10.5487/TR.2014.30.3.179
- Pisa LW, Amaral-Rogers V, Belzunces LP, Bonmatin JM, Downs A, Goulson D, Kreutzweiser DP, Krupke C, Liess M, McField M, Morrissey CA, Noome DA, Settele J, Simon-Delso N, Stark JD, Van der Sluijs JP, Van Dyck P, Wiemers M (2015) Effects of neonicotinoids and fipronil on non-target invertebrates. Environ Sci Pollut Res 22:68. doi: 10.1007/s11356-014-3471-x CrossRefGoogle Scholar
- Porrini C, Sabatini AG, Girotti S, Fini F, Monaco L, Celli G, Bortolotti L, Ghini S (2003) The death of honey bees and environmental pollution by pesticides: the honey bees as biological indicators. B Insectol 56(1):147–152Google Scholar
- Sizer IW, Beers RF Jr (1952) A spectrophotometric method for measuring the breakdown of hydrogen peroxide by catalase. J Biol Chem 195:133–139Google Scholar