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Antimicrobial activity of aqueous extracts from four plants on bacterial isolates from periodontitis patients

Environmental Science and Pollution Research volume 24, pages 13394–13404 (2017)Cite this article

Abstract

Four aqueous extracts of different plant organs are the following: Artemisia herba-alba, Opuntia ficus-indica, Camellia sinensis and Phlomis crinita were evaluated against two bacterial strains: Porphyromonas gingivalis and Prevotella intermedia, which are implicated in periodontal diseases. By using a disc method, these plant extracts demonstrated powerful bacterial activity against these Gram-negative strains. The minimum inhibitory concentration values of the four plant extracts varied between 0.03 and 590.82 mg/ml for the microbes. Another assay using commercial antibiotics and antibacterials as positive controls was also conducted. Values obtained after statistical analysis of inhibition diameters of all plant extracts demonstrated that for P. gingivalis, the aqueous extracts of A. herba-alba and O. ficus-indica were most effective, followed by those of C. sinensis and P. crinita. For P. intermedia, aqueous extracts of O. ficus-indica and C. sinensis appeared to be more efficient with significantly different (P > 0.05) inhibition diameters, followed by those of O. ficus-indica and P. crinita. In summary, the statistical results reveal that these plant extracts exert stronger antibacterial activity on P. intermedia germ as compared to P. gingivalis.

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Acknowledgements

The authors would like to thank the DGRSDT/MESRS Algeria for financially supporting this research.

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Affiliations

  1. Ecole Nationale Polytechnique d’Alger, Laboratoire BIOGEP, B.P. 182-16200, El Harrach, Alger, Algeria

    Leila Arbia, Nassima Chikhi-Chorfi, Selma Zenia, Nabil Mameri & Hakim Lounici

  2. Ecole Nationale Superieure Veterinaire d’Alger, BP 161, 16200, El Harrach, Alger, Algeria

    Nassima Chikhi-Chorfi

  3. Institut Pasteur d’Algérie, Service des Anaérobies, Route du petit staouéli, Dély-Brahim, Algeria

    Ilhem Betatache

  4. Laboratoire de Toxicologie, Faculté de Pharmacie, Université de Paris V, 4 avenus de l’Observatoire, 75006, Paris, France

    Chuong Pham-Huy

  5. Département Génie chimique, Université de Technologie de Compiègne, B.P. 20.509, 60205, Compiègne Cedex, France

    Nabil Mameri

  6. Université de Bouira, Bouira, Algeria

    Hakim Lounici

  7. Centre de Recherche en Technologie des Semi-conducteurs de l ‘Energetique (crtse), Algiers, Algeria

    Nadjib Drouiche

  8. Department of Environmental Engineering 2, Technology of Semi-conductor for the Energetic Research Center, Bd Frantz Fanon BP140 Alger-7-merveilles, 16038, Algiers, Algeria

    Nadjib Drouiche

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  1. Leila Arbia
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  2. Nassima Chikhi-Chorfi
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  3. Ilhem Betatache
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  4. Chuong Pham-Huy
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  5. Selma Zenia
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  6. Nabil Mameri
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  8. Hakim Lounici
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Correspondence to Nadjib Drouiche.

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Arbia, L., Chikhi-Chorfi, N., Betatache, I. et al. Antimicrobial activity of aqueous extracts from four plants on bacterial isolates from periodontitis patients. Environ Sci Pollut Res 24, 13394–13404 (2017). https://doi.org/10.1007/s11356-017-8942-4

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  • DOI: https://doi.org/10.1007/s11356-017-8942-4

Keywords

  • Artemisia herba-alba
  • Opuntia ficus-indica
  • Camellia sinensis
  • Phlomis crinita
  • Antibacterial activity
  • Porphyromonas gingivalis
  • Prevotella intermedia