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Exploring the Legionella pneumophila positivity rate in hotel water samples from Antalya, Turkey

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Abstract

The genus Legionella is a fastidious Gram-negative bacteria widely distributed in natural waters and man made water supply systems. Legionella pneumophila is the aetiological agent of approximately 90% of reported Legionellosis cases, and serogroup 1 is the most frequent cause of infections. Legionnaires’ disease is often associated with travel and continues to be a public health concern at present. The correct water management quality practices and rapid methods for analyzing Legionella species in environmental water is a key point for the prevention of Legionnaires’ disease outbreaks. This study aimed to evaluate the positivity rates and serotyping of Legionella species from water samples in the region of Antalya, Turkey, which is an important tourism center. During January−December 2010, a total of 1403 samples of water that were collected from various hotels (n = 56) located in Antalya were investigated for Legionella pneumophila. All samples were screened for L. pneumophila by culture method according to “ISO 11731-2” criteria. The culture positive Legionella strains were serologically identified by latex agglutination test. A total of 142 Legionella pneumophila isolates were recovered from 21 (37.5%) of 56 hotels. The total frequency of L. pneumophila isolation from water samples was found as 10.1%. Serological typing of 142 Legionella isolates by latex agglutination test revealed that strains belonging to L. pneumophila serogroups 2−14 predominated in the examined samples (85%), while strains of L. pneumophila serogroup 1 were less numerous (15%). According to our knowledge, our study with the greatest number of water samples from Turkey demonstrates that L. pneumophila serogroups 2−14 is the most common isolate. Rapid isolation of L. pneumophila from environmental water samples is essential for the investigation of travel related outbreaks and the possible resources. Further studies are needed to have epidemiological data and to determine the types of L. pneumophila isolates from Turkey.

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Authors and Affiliations

  1. Clinical Microbiology, Public Health Laboratory, Antalya, Turkey

    Nevgün Sepin Özen & Şenay Tuğlu Ataman

  2. Epidemiology, Public Health Laboratory, Antalya, Turkey

    Mestan Emek

Authors
  1. Nevgün Sepin Özen
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  2. Şenay Tuğlu Ataman
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  3. Mestan Emek
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Correspondence to Nevgün Sepin Özen.

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Responsible editor: Philippe Garrigues

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Sepin Özen, N., Tuğlu Ataman, Ş. & Emek, M. Exploring the Legionella pneumophila positivity rate in hotel water samples from Antalya, Turkey. Environ Sci Pollut Res 24, 12238–12242 (2017). https://doi.org/10.1007/s11356-017-8864-1

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  • DOI: https://doi.org/10.1007/s11356-017-8864-1

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