Environmental Science and Pollution Research

, Volume 24, Issue 9, pp 8790–8804 | Cite as

Biohydrogen production from sugarcane bagasse hydrolysate: effects of pH, S/X, Fe2+, and magnetite nanoparticles

  • Karen Reddy
  • Mahmoud Nasr
  • Sheena Kumari
  • Santhosh Kumar
  • Sanjay Kumar Gupta
  • Abimbola Motunrayo Enitan
  • Faizal Bux
Research Article

Abstract

Batch dark fermentation experiments were conducted to investigate the effects of initial pH, substrate-to-biomass (S/X) ratio, and concentrations of Fe2+ and magnetite nanoparticles on biohydrogen production from sugarcane bagasse (SCB) hydrolysate. By applying the response surface methodology, the optimum condition of steam-acid hydrolysis was 0.64% (v/v) H2SO4 for 55.7 min, which obtained a sugar yield of 274 mg g−1. The maximum hydrogen yield (HY) of 0.874 mol (mol glucose−1) was detected at the optimum pH of 5.0 and S/X ratio of 0.5 g chemical oxygen demand (COD, g VSS−1). The addition of Fe2+ 200 mg L−1 and magnetite nanoparticles 200 mg L−1 to the inoculum enhanced the HY by 62.1% and 69.6%, respectively. The kinetics of hydrogen production was estimated by fitting the experimental data to the modified Gompertz model. The inhibitory effects of adding Fe2+ and magnetite nanoparticles to the fermentative hydrogen production were examined by applying Andrew’s inhibition model. COD mass balance and full stoichiometric reactions, including soluble metabolic products, cell synthesis, and H2 production, indicated the reliability of the experimental results. A qPCR-based analysis was conducted to assess the microbial community structure using Enterobacteriaceae, Clostridium spp., and hydrogenase-specific gene activity. Results from the microbial analysis revealed the dominance of hydrogen producers in the inoculum immobilized on magnetite nanoparticles, followed by the inoculum supplemented with Fe2+ concentration.

Graphical abstract

Keywords

Biohydrogen Dark fermentation Full stoichiometry Hydrogen-producing microbes Magnetite nanoparticles Sugarcane bagasse hydrolysate 

Supplementary material

11356_2017_8560_MOESM1_ESM.docx (3.1 mb)
ESM 1(DOCX 3163 kb)
11356_2017_8560_MOESM2_ESM.docx (23 kb)
ESM 2(DOCX 22 kb)

References

  1. Akutsu Y, Lee D-Y, Li Y-Y, Noike T (2009) Hydrogen production potentials and fermentative characteristics of various substrates with different heat-pretreated natural microflora. Internatl J Hydrog Energy 34:5365–5372CrossRefGoogle Scholar
  2. Anam K, Habibi MS, Harwati TU, Susilaningsih D (2012) Photofermentative hydrogen production using Rhodobium marinum from bagasse and soy sauce wastewater. Internatl J Hydrog Energy 37:15436–15442CrossRefGoogle Scholar
  3. APHA (1998) Standard methods for the examination of water and wastewater, 20th ed. Washington, DC, USA: American Public Health Association/American Water Works Association/Water Environment FederationGoogle Scholar
  4. Ateia M, Nasr M, Ikeda A, Okada H, Fujii M, Natsuike M, Yoshimura C (2016) Nonlinear relationship of near-bed velocity and growth of riverbed periphyton. Water 8(461):1–12Google Scholar
  5. Beckers L, Hiligsmann S, Lambert SD, Heinrichs B, Thonart P (2013) Improving effect of metal and oxide nanoparticles encapsulated in porous silica on fermentative biohydrogen production by Clostridium butyricum. Bioresour Technol 133:109–117CrossRefGoogle Scholar
  6. Chang JJ, Chen WE, Shih SY, Yu SJ, Lay JJ, Wen FS, Huang CC (2006) Molecular detection of the clostridia in an anaerobic biohydrogen fermentation system by hydrogenase mRNA-targeted reverse transcription-PCR. Appl Microbiol Biotechnol 70:598–604CrossRefGoogle Scholar
  7. Chen CC, Lin CY, Chang JS (2001) Kinetics of hydrogen production with continuous anaerobic cultures utilizing sucrose as the limiting substrate. Appl Microbiol Biotechnol 57:56–64CrossRefGoogle Scholar
  8. Das D, Dutta T, Nath K, Kotay SM, Das AK, Veziroglu TN (2006) Role of Fe-hydrogenase in biological hydrogen production. Curr Sci 90:1627–1637Google Scholar
  9. de Sá LRV, de Oliveira TC, dos Santos TF, Matos A, Cammarota MC, Oliveira EMM, Ferreira-Leitão VS (2011) Hydrogenase activity monitoring in the fermentative hydrogen production using heat pretreated sludge: a useful approach to evaluate bacterial communities performance (technical communication). Internatl J Hydrog Energy 36:7543–7549CrossRefGoogle Scholar
  10. Fangkum A, Reungsang A (2011) Biohydrogen production from sugarcane bagasse hydrolysate by elephant dung: effects of initial pH and substrate concentration. Internatl J Hydrog Energy 36(14):8687–8696CrossRefGoogle Scholar
  11. Fazzeli H, Arabestani MR, Esfahani BN, Khorvash F, Pourshafie MR, Moghim S, Safaei HG, Faghri J, Narimani T (2012) Development of PCR-based method for detection of Enterobacteriaceae in septicemia. J Res Med Sci 17:671–675Google Scholar
  12. Fengel D, Wegener G (1984) Wood. Chemistry, ultrastructure, reactions. Berlin: Ed: Walter de GruyterGoogle Scholar
  13. Gadhe J, Gupta R (2007) Hydrogen production by methanol reforming in supercritical water: Catalysis by in-situ-generated copper nanoparticles. Internatl J Hydrog Energy 32:2374–2381Google Scholar
  14. Gadhe A, Sonawane S, Varma M (2015) Enhancement effect of hematite and nickel nanoparticles on biohydrogen production from dairy wastewater. Internatl J Hydrog Energy 13:4502–4511CrossRefGoogle Scholar
  15. Gupta R, Sharma K, Kuhad R (2009) Separate hydrolysis and fermentation (SHF) of Prosopis juliflora a wood substrate, for the production of cellulosic ethanol by Saccharomyces cerevisiae and Pichia stipitis—NCIM 3498. Bioresour Technol 100:1214–1220CrossRefGoogle Scholar
  16. Han H, Cui M, Wei L, Yang H, Shen J (2011) Enhancement effect of hematite nanoparticles on fermentative hydrogen production. Bioresour Technol 102:7903–7909CrossRefGoogle Scholar
  17. Lay J (2000) Modeling and optimization of anaerobic digested sludge converting starch to hydrogen. Biotechnol Bioeng 68:269–278CrossRefGoogle Scholar
  18. Lee H-S, Rittmann BE (2009) Evaluation of metabolism using stoichiometry in fermentative biohydrogen. Biotechnol Bioeng 102:749–758CrossRefGoogle Scholar
  19. Li J, Ren N (1998) The operational controlling strategy about the optimal fermentation type of acidogenic phase. Chin J Environmental Sci 18:398–402Google Scholar
  20. Logan B, Oh S, Kim I, Van Ginkel S (2002) Biological hydrogen production measured in batch anaerobic respirometers. Environmental Sci Technol 36:2530–2535CrossRefGoogle Scholar
  21. Mohanraj S, Kodhaiyolli S, Rengasamy M, Pugalenthi V (2014) Phytosynthesized iron oxide nanoparticles and ferrous iron on fermentative hydrogen production using Enterobacter cloacae: evaluation and comparison of the effects. Internatl J Hydrog Energy 39:11920–11929CrossRefGoogle Scholar
  22. Mullai P, Yogeswari M, Sridevi K (2013) Optimisation and enhancement of biohydrogen production using nickel nanoparticles—a novel approach. Bioresour Technol 141:212–219CrossRefGoogle Scholar
  23. Nasr M, Tawfik A, Ookawara S, Suzuki M (2013a) Biological hydrogen production from starch wastewater using a novel up-flow anaerobic staged reactor. BioResour 8:4951–4968CrossRefGoogle Scholar
  24. Nasr M, Tawfik A, Ookawara S, Suzuki M (2013b) Environmental and economic aspects of hydrogen and methane production from starch wastewater industry. J Water Environment Technol 11:463–475CrossRefGoogle Scholar
  25. Nasr M, Tawfik A, Ookawara S, Suzuki M, Kumari S, Bux F (2015) Continuous biohydrogen production from starch wastewater via sequential dark-photo fermentation with emphasize on maghemite nanoparticles. J Industrial Eng Chem 21:500–506CrossRefGoogle Scholar
  26. Nath K, Muthukumar M, Kumar A, Das D (2008) Kinetics of two-stage fermentation process for the production of hydrogen. Internatl J Hydrog Energy 33:1195–1203CrossRefGoogle Scholar
  27. Oh SE, Van Ginkel S, Logan BE (2003) The relative effectiveness of pH control and heat treatment for enhancing biohydrogen gas production. Environ Sci Technol 37:5186–5190CrossRefGoogle Scholar
  28. Pattra S, Sangyoka S, Boonmee M, Reungsang A (2008) Biohydrogen production from the fermentation of sugarcane bagasse hydrolysate by Clostridium butyricum. Internatl J Hydrog Energy 33:6058–6065CrossRefGoogle Scholar
  29. Peters JW, Schut GJ, Boyd ES, Mulder DW, Shepard EM, Broderick JB, King PW, Adams MWW (2015) [FeFe]- and [NiFe]-hydrogenase diversity, mechanism, and maturation. Biochimica et Biophysica Acta (BBA) - Molecular Cell Research 1853:1350–1369CrossRefGoogle Scholar
  30. Rai PK, Singh SP, Asthana RK, Singh S (2014) Biohydrogen production from sugarcane bagasse by integrating dark- and photo-fermentation. Bioresour Technol 152:140–146CrossRefGoogle Scholar
  31. Rittmann BE, McCarty PL (2001) Environmental biotechnology: principles and applications. New York: McGraw-HillGoogle Scholar
  32. Seviour R, Nielsen P (2010) Microbial ecology of activated sludge. London, United Kingdom: IWA PublishingGoogle Scholar
  33. Singh L, Siddiqui MF, Ahmad A, MH AR, Sakinah M, Wahid ZA (2013) Biohydrogen production from palm oil mill effluent using immobilized mixed culture. J Industrial Eng Chem 19:659–664CrossRefGoogle Scholar
  34. Tan C, Ma F, Qiu S (2013) Impact of carbon to nitrogen ratio on nitrogen removal at a low oxygen concentration in a sequencing batch biofilm reactor. Water Sci Technol 67:612–618CrossRefGoogle Scholar
  35. Tolvanen KES, Santala VP, Karp MT (2010) [FeFe]-hydrogenase gene quantification and melting curve analysis from hydrogen-fermenting bioreactor samples. Internatl J Hydrog Energy 35:3433–3439CrossRefGoogle Scholar
  36. Wang J, Wan W (2008) Effect of Fe2+ concentration on fermentative hydrogen production by mixed cultures. Internatl J Hydrog Energy 33:1215–1220CrossRefGoogle Scholar
  37. Wang X, Hoefel D, Saint CP, Monis PT, Jin B (2007) The isolation and microbial community analysis of hydrogen producing bacteria from activated sludge. J Appl Microbiol 103:1415–1423CrossRefGoogle Scholar
  38. Winsor C (1932) The Gompertz curve as a growth curve. Proceedings of the National Academy of Sciences USA 18:1–8CrossRefGoogle Scholar
  39. Zhang Y, Liu G, Shen J (2005) Hydrogen production in batch culture of mixed bacteria with sucrose under different iron concentrations. Internatl J Hydrog Energy 30:855–860CrossRefGoogle Scholar
  40. Zhang Y, Shen J (2007) Enhancement effect of gold nanoparticles on biohydrogen production from artificial wastewater. Internatl J Hydrog Energy 32:17–23CrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 2017

Authors and Affiliations

  • Karen Reddy
    • 1
  • Mahmoud Nasr
    • 2
  • Sheena Kumari
    • 1
  • Santhosh Kumar
    • 3
  • Sanjay Kumar Gupta
    • 1
  • Abimbola Motunrayo Enitan
    • 1
  • Faizal Bux
    • 1
  1. 1.Institute for Water and Wastewater TechnologyDurban University of TechnologyDurbanSouth Africa
  2. 2.Sanitary Engineering Department, Faculty of EngineeringAlexandria UniversityAlexandriaEgypt
  3. 3.Department of Biotechnology and Food TechnologyDurban University of TechnologyDurbanSouth Africa

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