Abstract
Pseudomonas aeruginosan has emerged as an important pathogen elated to serious infections and nosocomial outbreaks worldwide. This study was conducted to understand the prevalence of aminoglycoside (AMG)-resistant P. aeruginosa in our hospital and to provide a scientific basis for control measures against nosocomial infections. Eighty-two strains of P. aeruginosa were isolated from clinical departments and divided into AMG-resistant strains and AMG-sensitive strains based on susceptibility test results. AMG-resistant strains were typed by drug resistance gene typing (DRGT) and protein typing. Five kinds of aminoglycoside-modifying enzyme (AME) genes were detected in the AMG-resistant group. AMG-resistant P. aeruginosa strains were classified into three types and six subtypes by DRGT. Four protein peaks, namely, 9900.02, 7600.04, 9101.25 and 10,372.87 Da, were significantly and differentially expressed between the two groups. AMG-resistant P. aeruginosa strains were also categorised into three types and six subtypes at the distance level of 10 by protein typing. AMG-resistant P. aeruginosa was cloned spread in our hospital; the timely implementation of nosocomial infection prevention and control strategies were needed in preventing outbreaks and epidemic of AMG-resistant P. aeruginosa. SELDI-TOF MS technology can be used for bacterial typing, which provides a new method of clinical epidemiological survey and nosocomial infection control.
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Abbreviations
- AMG:
-
Aminoglycoside
- DRGT:
-
Drug resistance gene typing
- AME:
-
Aminoglycoside-modifying enzyme
- SELDI-TOF MS:
-
Surface-enhanced laser desorption/ionization-time of flight mass spectrometry
- MDR:
-
Multidrug-resistant
- APH:
-
Aminoglycoside phosphoryltransferase
- AAC:
-
Aminoglycoside acetyltransferase
- ANT:
-
Aminoglycoside nucleotidyl transferase
- AFLP:
-
Amplified fragment length polymorphism
- RFLP:
-
Restriction fragment length polymorphism
- REP-PCR:
-
Repetitive extragenic palindromic polymerase chain reaction
- PFGE:
-
Pulsed-field gel electrophoresis
- MLST:
-
Multilocus sequence typing
- CLSI:
-
Clinical Laboratory Standards Institute
- MICs:
-
Minimal inhibitory concentrations
- ACTH:
-
Adrenocorticotropic hormone
- MALDI:
-
Matrix-assisted laser desorption and ionization
References
Allegranzi B, Bagheri NS, Combescure C et al (2011) Burden of endemic health-care-associated infection in developing countries: systematic review and meta-analysis. Lancet 377(9761):228–241. https://doi.org/10.1016/S0140-6736(10)61458-4
Bongat AF, Saksena R, Adamo R et al (2010) Multimeric bivalent immunogens from recombinant tetanus toxin HC fragment, synthetic hexasaccharides, and a glycopeptide adjuvant. Glycoconj J 27(1):69–77. https://doi.org/10.1007/s10719-009-9259-4
Cheng K, Chui H, Domish L, Hernandez D, Wang G (2016) Recent development of mass spectrometry and proteomics applications in identification and typing of bacteria. Proteomics Clin Appl 10(4):346–357. https://doi.org/10.1002/prca.201500086
Fenselau CC (2013) Rapid characterization of microorganisms by mass spectrometry—what can be learned and how. J Am Soc Mass Spectrom 24(8):1161–1166. https://doi.org/10.1007/s13361-013-0660-7
Heister T, Kaier K, Wolkewitz M (2017) Estimating the burden of nosocomial infections: time dependency and cost clustering should be taken into account. Am J Infect Control 45(1):94–95. https://doi.org/10.1016/j.ajic.2016.07.030
Jia X, Zhang J, Sun W, He W, Jiang H, Chen D, Murchie AIH (2013) Riboswitch control of aminoglycoside antibiotic resistance. Cell 152(1-2):68–81. https://doi.org/10.1016/j.cell.2012.12.019
Jin H, XM X, Mi ZH et al (2009) Drug-resistant gene based genotyping for Acinetobacter baumannii in tracing epidemiological events and for clinical treatment within nosocomial settings. Chin Med J 122(3):301–306
Li W, Raoult D, Fournier PE (2009) Bacterial strain typing in the genomic era. FEMS Microbiol Rev 33(5):892–916. https://doi.org/10.1111/j.1574-6976.2009.00182.x
Li XQ, Huang WF, Liu H, et al (2012) Establishment of protein fingerprint database of Salmonella paratyphi A using SELDI-TOF-MS. Chin J Cell Mol Immunol 28(6):576–579
Livermore DM (2002) Multiple mechanisms of antimicrobial resistance in Pseudomonas aeruginosa: our worst nightmare. Clin Infect Dis 34(5):634–640. https://doi.org/10.1086/338782
McFarland MA, Andrzejewski D, Musser SM, Callahan JH (2014) Platform for identification of Salmonella serovar differentiating bacterial proteins by top-down mass spectrometry: S. Typhimurium vs S. Heidelberg. Anal Chem 86(14):6879–6886. https://doi.org/10.1021/ac500786s
Mei-jie JIANG, Li FENG, Shu-ping ZHAO (2009) Multi-drug resistant genes of Pseudomonas aeruginosa clinical strains. Chinese J Nosocomiology 19:8–12 in Chinese
Moore NM, Flaws ML (2011) Antimicrobial resistance mechanisms in Pseudomonas aeruginosa. Clin Lab Sci 24(1):47–51
Odumosu BT, Adeniyi BA, Chandra R (2015) Occurrence of aminoglycoside-modifying enzymes genes (aac(6′)-I and ant(2″)-I) in clinical isolates of Pseudomonas aeruginosa from Southwest Nigeria. Afr Health Sci 15(4):1277–1281. https://doi.org/10.4314/ahs.v15i4.29
Over U, Gur D, Unal S, Miller GH (2001) The changing nature of aminoglycoside resistance mechanisms and prevalence of newly recognized resistance mechanisms in Turkey. Clin Microbiol Infect 7(9):470–478. https://doi.org/10.1046/j.1198-743x.2001.00284.x
Poonsuk K, Tribuddharat C, Chuanchuen R (2013) Aminoglycoside resistance mechanisms in Pseudomonas aeruginosa isolates from non-cystic fibrosis patients in Thailand. Can J Microbiol 59(1):51–56. https://doi.org/10.1139/cjm-2012-0465
Quiroga MP, Andres P, Petroni A, Soler Bistue AJC, Guerriero L, Vargas LJ, Zorreguieta A, Tokumoto M, Quiroga C, Tolmasky ME, Galas M, Centron D (2007) Complex class 1 integrons with diverse variable regions, including aac(6′)-Ib-cr, and a novel allele, qnrB10, associated with ISCR1 in clinical enterobacterial isolates from Argentina. Antimicrob Agents Chemother 51(12):4466–4470. https://doi.org/10.1128/AAC.00726-07
Ramirez MS, Tolmasky ME (2010) Aminoglycoside modifying enzymes. Drug Resist Updat 13(6):151–171. https://doi.org/10.1016/j.drup.2010.08.003
Ramírez MS, Quiroga C, Centrón D (2005) Novel rearrangement of a class 2 integron in two non-epidemiologically related isolates of Acinetobacter baumannii. Antimicrob Agents Chemother 49(12):5179–5181. https://doi.org/10.1128/AAC.49.12.5179-5181.2005
Shakil S, Khan R, Zarrilli R, Khan AU (2008) Aminoglycosides versus bacteria—a description of the action, resistance mechanism, and nosocomial battleground. J Biomed Sci 15(1):5–14. https://doi.org/10.1007/s11373-007-9194-y
Shaw KJ, Rather PN, Hare RS, Miller GH (1993) Molecular genetics of aminoglycoside resistance genes and familial relationships of the aminoglycoside-modifying enzymes. Microbiol Rev 57(1):138–163
Singh A, Goering RV, Simjee S, Foley SL, Zervos MJ (2006) Application of molecular techniques to the study of hospital infection. Clin Microbiol Rev 19(3):512–530. https://doi.org/10.1128/CMR.00025-05
Vakulenko SB, Mobashery S (2003) Versatility of aminoglycosides and prospects for their future. Clin Microbiol Rev 16(3):430–450. https://doi.org/10.1128/CMR.16.3.430-450.2003
Vaziri F, Peerayeh SN, Nejad QB, Farhadian A (2011) The prevalence of aminoglycoside-modifying enzyme genes (aac (6′)-I, aac (6′)-II, ant (2″)-I, aph (3′)-VI) in Pseudomonas aeruginosa. Clinics (Sao Paulo) 66(9):1519–1522
Wu X, Yan T, Liu Y, Wang J, Li Y, Wang S (2016) Nosocomial infections among acute leukemia patients in China: an economic burden analysis. Am J Infect Control 44(10):1123–1127. https://doi.org/10.1016/j.ajic.2016.03.072
Xiao D, Yang Y, Jiang W, Zhang H, Liu H, Yu H, Xie C, Zhong M, Chen L, Huang W (2014) Direct common gram-negative bacterial identification from positive blood culture bottles by SELDI-TOF MS. J Microbiol Methods 105:116–120. https://doi.org/10.1016/j.mimet.2014.07.021
Xu P, Alam MM, Kalsy A, Charles RC, Calderwood SB, Qadri F, Ryan ET, Kováč P (2011) Simple, direct conjugation of bacterial O-SP-core antigens to proteins: development of cholera conjugate vaccines. Bioconjug Chem 22(10):2179–2185. https://doi.org/10.1021/bc2001984
Yan Q, Chong Z, Kai-yuan C et al (2012) Researches on the correlated resistant genes of aminoglycosides-resistant Pseudomonas aerugionsa. Chinese J Antibiotics 37:501–506 in Chinese
Yun LI, Yuan LV, Bo ZHENG (2012) Ministry of Health National Antimicrobial Resistance Investigation Net annual report of 2011:surveillance of antimicrobial resistance in nonfermenting gram-negative bacteria in China. The Chinese Journal of Clinical Pharmacology 28:883–887 in Chinese
Funding
This research was funded by the National Natural Fund Project of China (No. 41472046), the Key Program of National Natural Science Project of China (No. 41130746), the Science and Technology Project of Sichuan Province, China (No. 2016JY0045), the Strategic Cooperation Project of the Luzhou People’s Government and Sichuan University, China (No. 2013CDLZ-S15) and the Fund Project of the Science and Technology Leader of Sichuan Province (No. 15031).
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Song, M., Tang, M., Ding, Y. et al. Application of protein typing in molecular epidemiological investigation of nosocomial infection outbreak of aminoglycoside-resistant Pseudomonas aeruginosa . Environ Sci Pollut Res 25, 22437–22445 (2018). https://doi.org/10.1007/s11356-017-0960-8
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DOI: https://doi.org/10.1007/s11356-017-0960-8