Effects of organochlorine pesticides on plant growth-promoting traits of phosphate-solubilizing rhizobacterium, Paenibacillus sp. IITISM08

Rani, Rupa; Usmani, Zeba; Gupta, Pratishtha; Chandra, Avantika; Das, Aakankshya; Kumar, Vipin

doi:10.1007/s11356-017-0940-z

Research Article
Published: 11 December 2017

Effects of organochlorine pesticides on plant growth-promoting traits of phosphate-solubilizing rhizobacterium, Paenibacillus sp. IITISM08

Rupa Rani¹,
Zeba Usmani¹,
Pratishtha Gupta¹,
Avantika Chandra²,
Aakankshya Das³ &
Vipin Kumar ORCID: orcid.org/0000-0002-1077-3440¹

Environmental Science and Pollution Research volume 25, pages 5668–5680 (2018)Cite this article

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Abstract

The study aimed to identify an effective phosphate-solubilizing and organochlorine pesticide-tolerant bacterial strain(s). A total of 50 phosphate-solubilizing bacterial (PSB) strains were isolated from pesticide-stressed soil. Ten isolates showing higher solubilization were selected for organochlorine pesticides (endosulfan, aldrin, and lindane) tolerance. The strain IITISM08 showed the maximum potential of phosphorous solubilization in Pikovaskya agar medium (solubilization index = 3.2) and in broth medium (348 ± 2 μg mL⁻¹) and tolerated up to 250 μg mL⁻¹ of organochlorine pesticides. During phosphorous solubilization, the presence of functional group and organic acid production were also observed using FT-IR and HPLC. The plant growth-promoting (PGP) traits of the strain IITISM08 was highly inhibited in presence of endosulfan among the three organochlroine pesticides. The strain IITISM08 degraded aldrin (79%), lindane (68%), and endosulfan (51%) at a concentration of 50 μg mL⁻¹. The strain IITISM08 was identified using 16S rDNA gene sequencing as Paenibacillus sp. (IITISM08). The study revealed that the strain IITISM08 can be used as PGP candidate even under organochlorine pesticide-stressed condition.

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Laboratory of Applied Microbiology, Department of Environmental Science and Engineering, Indian Institute of Technology (Indian School of Mines), Dhanbad, Jharkhand, 826004, India
Rupa Rani, Zeba Usmani, Pratishtha Gupta & Vipin Kumar
Applied Air and Soil Laboratory, Department of Environmental Science and Engineering, Indian Institute of Technology (Indian School of Mines), Dhanbad, Jharkhand, 826 004, India
Avantika Chandra
ICAR-Central Institute of Freshwater Aquaculture (CIFA), Bhubaneswar, India
Aakankshya Das

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Rupa Rani
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Zeba Usmani
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Pratishtha Gupta
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Avantika Chandra
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Aakankshya Das
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Vipin Kumar
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Correspondence to Vipin Kumar.

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Rani, R., Usmani, Z., Gupta, P. et al. Effects of organochlorine pesticides on plant growth-promoting traits of phosphate-solubilizing rhizobacterium, Paenibacillus sp. IITISM08. Environ Sci Pollut Res 25, 5668–5680 (2018). https://doi.org/10.1007/s11356-017-0940-z

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Received: 13 June 2017
Accepted: 04 December 2017
Published: 11 December 2017
Issue Date: February 2018
DOI: https://doi.org/10.1007/s11356-017-0940-z

Keywords

Phosphorous-solubilizing bacteria
Organochlorine pesticides
Plant growth-promoting traits
Pesticide-resistant bacteria
Rhizospheric soil