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Mucociliary transport, differential white blood cells, and cyto-genotoxicity in peripheral erythrocytes in fish from a polluted urban pond

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Abstract

The present study evaluated the water quality of a polluted pond through the analysis of in vitro mucociliary transport, hematological parameters, and biomarkers of cyto-genotoxicity in the Nile tilapia (Oreochromis niloticus). Blood and mucus samples were collected from ten specimens from the polluted pond and from ten specimens from a control area. The fish were anesthetized with 3% benzocaine, mucus was collected directly from the gills, and blood was drawn from the caudal artery. Blood smears were stained using the May-Grünwald Giemsa process for the differential leukocyte counts and to determine the frequency of leukocytes, thrombocytes, erythroblasts, micronuclei, and nuclear abnormalities. The results revealed low transportability in vitro, a high percentage of monocytes and eosinophils, and increased frequency of leukocytes and nuclear abnormalities in fish from the polluted pond. However, the frequency of thrombocytes and erythroblasts and the percentage of lymphocytes and neutrophils were significantly lower. It is possible to conclude that changes in fish are due to poor water quality and that these non-destructive biomarkers can be used for the biomonitoring of aquatic environments vulnerable to contamination.

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Acknowledgments

The authors would like to thank Dr. Rogerio Pazzeti for providing the frog palate and Dr. Mariângela Macchione for her help during frog palate preparation sampling. The authors are also grateful to Mrs. Sonia A.S. Feliciano and Mr. Guilherme F. Cavalheiro for their assistance in laboratorial procedures and to vivarium technicians Mr. Luiz Afonso, Mr. Davi Francisco, and Mr. Ivandir. D.M.S. Abessa thanks the Brazilian National Council for Scientific and Technological Development (CNPq) for the financial support. The authors would also like to thank Danielle Deremo Cosimo of Superior Translation Services for reviewing the manuscript.

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Authors and Affiliations

  1. Pós-Graduacão em Ecogestão, Campus Paraíso, Universidade Paulista—UNIP, São Paulo, Brazil

    Edison Bezerra da Silva & Sandra Aparecida da Silva Corrêa

  2. Escola Municipal de Ensino Fundamental Olavo Fontoura, Prefeitura do Município de São Paulo, São Paulo, Brazil

    Edison Bezerra da Silva

  3. Campus do Litoral Paulista—Núcleo de Estudos em Poluição e Ecotoxicologia Aquática, Universidade Estadual Paulista—UNESP, São Vicente, Brazil

    Denis Moledo de Souza Abessa

  4. Faculdade das Américas—FAM, São Paulo, Brazil

    Bruno Ferreira Xavier da Silva, Dolores Helena Rodriguez Ferreira Rivero & Robson Seriani

  5. Departamento de Patologia, Faculdade de Medicina, Laboratório de Investigação Médica LIM05, Universidade de São Paulo, Av. Dr. Arnaldo 455, 1°andar, sala 1150, Cerqueira César, São Paulo, Brazil

    Dolores Helena Rodriguez Ferreira Rivero & Robson Seriani

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  1. Edison Bezerra da Silva
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Correspondence to Robson Seriani.

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da Silva, E.B., da Silva Corrêa, S.A., de Souza Abessa, D.M. et al. Mucociliary transport, differential white blood cells, and cyto-genotoxicity in peripheral erythrocytes in fish from a polluted urban pond. Environ Sci Pollut Res 25, 2683–2690 (2018). https://doi.org/10.1007/s11356-017-0729-0

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  • DOI: https://doi.org/10.1007/s11356-017-0729-0

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