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Ovarian growth impairment after chronic exposure to Roundup Ultramax® in the estuarine crab Neohelice granulata

Abstract

Adult females of the estuarine crab Neohelice granulata were exposed to the glyphosate formulation Roundup Ultramax® during the entire 3-month pre-reproductive period. At the end of the assay, a significant higher increment of glycemia was noted at both glyphosate concentrations assayed (0.01 and 0.2 mg/L, acid equivalent). Although no differences were observed in the gonadosomatic index, a significantly higher proportion of reabsorbed vitellogenic oocyte was observed at the highest glyphosate concentration, together with a significant decrease of vitellogenin content in the ovary. In addition, some in vitro assays were carried out by co-incubating small pieces of ovary with or without the addition of Roundup; at both concentrations tested (same as those used in vivo), a decrease in the ovarian vitellogenin content was observed, whereas the ovarian protein synthesis was significantly inhibited by glyphosate at 0.2 mg/L in the Roundup formulation used.

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Acknowledgements

This study was supported by grants from the University of Buenos Aires (UBACYT 2016–2018, code 20020150100060BA) and CONICET (PIP 2015, code 11220150100100CO).

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Correspondence to Enrique M. Rodríguez.

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Responsible editor: Philippe Garrigues

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Canosa, I.S., Silveyra, G.R., Avigliano, L. et al. Ovarian growth impairment after chronic exposure to Roundup Ultramax® in the estuarine crab Neohelice granulata . Environ Sci Pollut Res 25, 1568–1575 (2018). https://doi.org/10.1007/s11356-017-0581-2

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  • DOI: https://doi.org/10.1007/s11356-017-0581-2

Keywords

  • Roundup
  • Glyphosate
  • Crabs
  • Ovary
  • Reproduction
  • Vitellogenin