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Assessment of protective potential of Nigella sativa oil against carbendazim- and/or mancozeb-induced hematotoxicity, hepatotoxicity, and genotoxicity

Environmental Science and Pollution Research volume 25, pages 1270–1282 (2018)Cite this article

Abstract

Nigella sativa oil (NSO) possesses antioxidant activity. However, its protective role against the hazards of fungicides has been poorly studied. Therefore, the present work aimed at determining the ameliorative potential of NSO against hepatotoxicity induced by carbendazim (CBZ) and/or mancozeb (MNZ) in female rats. In the present study, about 120 adult female Sprague-Dawley rats were randomly divided into eight equal groups. One group of animals was kept as a negative control (Gp. 1); groups 2, 3 and 4 orally received CBZ (200 mg/kg body wt) and/or MNZ (300 mg/kg body wt) daily for 2 weeks (positive groups). In order to assess the hepatoprotective potential of NSO, in comparison with NSO-treated rats (Gp. 5), groups 6, 7 and 8 were CBZ- and/or MNZ-exposed groups pre-treated orally with NSO (2 ml/kg body wt) daily for 2 weeks (prophylactic groups). All groups were kept further for 15 days without medications to observe the withdrawal effect. At the end of exposure and withdrawal periods, the body weight of all experimental rats was recorded and blood samples were collected for hematological, clinico-biochemical, and micronucleus assays. The animals were then sacrificed, and the liver and bone marrow were harvested for oxidative stress bioassay, chromosomal aberrations, DNA fragmentation, and histopathological examinations. The results suggested that pre-treatment with NSO remarkably diminished CBZ- and MNZ-induced macrocytic hypochromic anemia, leukocytosis, lymphocytosis, eosinophilia, and neutropenia. Besides, it also minimized the elevated liver enzymes, lipid peroxidation, micronucleus incidence, DNA damage, and chromosomal aberration frequency. Conversely, NSO significantly stimulated the CBZ- and/or MNZ-induced antioxidant system suppression. The NSO also normalized the hepatic structural architecture. As far as withdrawal effect is concerned, there was almost disappearance of the bad effects of these fungicides and the values were close to the normal range especially with the use of NSO. Ultimately, the results revealed that N. sativa oil is an effective hepatoprotective agent due to its genoprotective and free radical scavenging activities.

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References

  • Aebi H (1984) Catalase in vitro. Methods Enzymol 105:121–126

    CAS  Article  Google Scholar 

  • Al Sa’aidi JAA, Dawood KHA, Latif AD (2012) Immunomodulatory effect of Nigella sativa seed extract in male rabbits treated with dexamethasone. Iraqi J of Vet Sci 26(IV):141

    Google Scholar 

  • Alenzi FQ, Alsakran Altamimi MA, Kujan O, Tarakji B, Tamimi W (2013) Antioxidant properties of Nigella sativa. J Mol Genet Med 7:77

    Article  Google Scholar 

  • Ananthan G, Kumaran B (2013) Effect of mancozeb on the specific activities of testicular phosphatases and protective role of vitamin C in albino rats. Bull Env Pharmacol Life Sci 2(7):56–61

    CAS  Google Scholar 

  • Asaduzzaman M, Nahar L, Rahman MS, Hasan M, Khatun A (2015) Hypoglycemic and hypolipidemic potential of Nigella sativa L. seed extract in streptozotocin (STZ)-induced diabetic rats. J Plant Biochem Physiol 3:158

    Google Scholar 

  • Atamanalp M, Nayik T (2003) Alterations in hematological parameters of rainbow trout (Oncorhynchus mykiss) exposed to mancozeb. Turk J Vet Anim Sci 27:1213–1217

  • Baligar PN, Kaliwal BB (2001) Induction of gonadal toxicity to female rat after chronic exposure to mancozeb. Ind Health 39:235–243

    CAS  Article  Google Scholar 

  • Barlas N, Selmanoglu G, Koçkaya A, Songür S (2002) Effects of carbendazim on rat thyroid, parathyroid, pituitary and adrenal glands and their hormones. Hum Exp Toxicol 21(4):217–221

    CAS  Article  Google Scholar 

  • Bertram C, Hass R (2008) Cellular responses to reactive oxygen species-induced DNA damage and aging. Biol Chem 389:211–220

    CAS  Article  Google Scholar 

  • Bukhari MH, Khalil J, Qamar S, Qamar Z, Zahid M, Ansari N (2011) Comparative gastroprotective effects of natural honey Nigella sativa and cimetidine against acetylsalicylic acid induced gastric ulcer in albino rats. J Coll Physicians Surg Pak 21:151–156

    Google Scholar 

  • Burits M, Bucar F (2000) Antioxidant activity of Nigella sativa essential oil. Phytother Res 14(14):323–328

    CAS  Article  Google Scholar 

  • Burtis CA, Ashwood ER (1994), editors. Tietz textbook of clinical chemistry. 2nd ed. Philadelphia, W.B.Saunders Company, 830–843

  • Calviello G, Piccioni E, Boninsegna A, Tedesco B, Maggiano N, Serini S, Wolf FI, Palozza P (2006) DNA damage and apoptosis induction by the pesticide mancozeb in rat cells: involvement of the oxidative mechanism. Toxicol and Appl Pharmacol 211:87–96

    CAS  Article  Google Scholar 

  • Castro S, Vinocur M, Permigiani M, Halle C, Taurian T, Fabra A (1997) Interaction of the fungicide Mancozeb and Rhizobium sp. in pure culture and under field conditions. Biol Fertil Soil 25:147–151

    CAS  Article  Google Scholar 

  • Cédric P, Sébastien V, Karim A, Philippe D, Marie-Hélène P, Marie-Roberte G, Philippe B, Odette P (2016) Ex vivo assessment of testicular toxicity induced by carbendazim and iprodione, alone or in a mixture. ALTEX 33(4)

  • Costa LG, Giordano G, Guizzetti M, Vitalone A (2008) Neurotoxicity of pesticides: a brief review. Front Biosci 1(13):1240–1249

    Article  Google Scholar 

  • Danladi J, Ahmed SA, Akpulu SP, Owolagba GK, Iduh MU (2013) Protective effect of cool extraction of black seed (Nigella sativa) oil against Ccl4-induced oxidative damages in Wistar rats testis. IOSR J Pharm Biol Sci 5:68–74

    Google Scholar 

  • Dollah MA, Parhizkar S, Latiff LA, Bin Hassan MH (2013) Toxicity effect of Nigella sativa on the liver function of rats. Adv Pharma Bulletin 3(1):97–102

    Google Scholar 

  • Ekanem JT, Yusuf OK (2008) Some biochemical and hematological effects of black seed (Nigella sativa) oil on T. brucei-infected rats. Afr J Biomed Res 11:79–85

    Google Scholar 

  • El-Damaty EMA, Farrag AH, Rowayshed G, Fahmy HM (2012) Biochemical and histopathological effects of systemic pesticides on some functional organs of male albino rats. J of Appl Sci Res 8(11):5459–5469

    CAS  Google Scholar 

  • Farrag AH, Mahdy KA, Abd El- Rahman JH, Osfor MM (2007) Protective effect of Nigella sativa seeds against lead induced hepatorenal damage in male rats. Pak J of Biol sci 10(7):2809–2816

    CAS  Google Scholar 

  • Feldman BF, Zinkl JG, Jain NC (2000) Schalm’s veterinary hematology, 5th edn. Lippincott Williams and Wilkins A Wolters Company Philadelphia, Baltimore, New York, London, pp 1219–1224

    Google Scholar 

  • Forouzanfar F, Bazzaz BSF, Hosseinzadeh H (2014) Black cumin (Nigella sativa) and its constituent (thymoquinone): A review on antimicrobial effects. Iran J Bas Med Sci 17(12):929–938

    Google Scholar 

  • Gani ANS, John SA (2013) Evalution of hepatoprotective effect of Nigella sativa L. Int. J Pharm Sci 5(4):428–430

    Google Scholar 

  • Gareeballa OA, Mahbulur R, Sei JL, Kim G, Hyung SK, Jin SK, Shang JK (2016) Hepatoprotective effects of Nigella sativa seed extract against acetaminophen-induced oxidative stress. Asian Pacific J of Trop Med 9(3):221–227

    Article  Google Scholar 

  • Ghada ZAS, Salia MH, Mohamed A (2012) Protective effect of Curcuma donga or Nigella sativa on aflatoxin B1-induced hepato-toxicity in rats in relation to food safety on public health. Med J Cairo Univ 80(2):191–203

    Google Scholar 

  • Goldner WS, Sandler DP, Yu F, Hoppin JA, Kamel F, Levan TD (2010) Pesticide use and thyroid disease among women in the Agricultural Health Study. Am J Epidemiol 171:455–464

    Article  Google Scholar 

  • Goldoni A, Klauck CR, Da Silva ST, Da Silva MD, Ardenghi PG, Da Silva LB (2014) DNA damage in wistar rats exposed to dithiocarbamate pesticide mancozeb. Folia Biologica (Praha) 60:202–204

    CAS  Google Scholar 

  • Halliwell B, Gutteridge JM (1990) Role of free radicals and catalytic metal ions in human disease: an overview. Methods Enzymol 186:1–85

    CAS  Article  Google Scholar 

  • Haq A, Abdullatif M, Lobo PI, Khabar KS, Sheth KV, Al-Sedairy ST (1995) Nigella sativa: effect on human lymphocytes and polymorphonuclear leukocyte phagocytic activity. Immunopharmacology 30:147–155

    CAS  Article  Google Scholar 

  • Hashem HE (2012) Light and electron microscopic study of the possible protective effect of Nigella sativa on metalaxyl induced hepatotoxicity in adult albino rats. J Cell Sci Ther 3:118

    Article  Google Scholar 

  • Ines BD, Alya A, Manel J, Soumaya M, Najoua G, Saloua E, Mohamed ML (2016) Carbamates pesticides induced immunotoxicity and carcinogenicity in human: a review. J Appl Biomed 14:85–90

    Article  Google Scholar 

  • Kackar R, Srivastava MK, Raizada RB (1999) Induction of gonadal toxicity to male rat after chronic exposure to mancozeb. Ind Health 35:104–111

    Article  Google Scholar 

  • Koller A (1984) Total serum protein. Kaplan A et al. Clin Chem The C.V. Mosby Co. St Louis. Toronto. Princeton ,418:1316–1324

  • Krystyna S (2001) Embryolethal and teratogenic effects of carbendazim in rats. Teratog Carcinog Mutag 21:335–340

    Article  Google Scholar 

  • Ksheerasagar RL, Kaliwal BB (2003) Temporal effects of mancozeb on testes, accessory reproductive organs and biochemical constituents in albino mice. Environ Toxicol and Pharmacol 15:9–17

    CAS  Article  Google Scholar 

  • Ksheerasagar RL, Kaliwal BB (2010) Effect of mancozeb on thyroid, testis, accessory reproductive organs and biochemical constituents in albinos mice. Rec Res in Sci Technol 2:7–17

    CAS  Google Scholar 

  • Kumar A, Tomar M, Kumar S, Maharshi K (2014) Effect of sub-lethal doses of imidacloprid on histological and biochemical parameters in female albino mice. ISOR J of Envir Sci Toxicol Food Techn 8:9–15

    CAS  Article  Google Scholar 

  • Larramendy ML, Nikoloff ML, de Arcaute CR, Soloneski S (2014) Genotoxicity and cytotoxicity exerted by pesticides in different biotic matrices. An overview of more than a decade of experimental evaluation. J Environ Anal Toxicol 4:225

    Google Scholar 

  • Li S, Cao C, Shi H, Yang S, Qi L, Zhao X, Sun C (2016) Effect of quercetin against mixture of four organophosphate pesticides induced nephrotoxicity in rats. Xenobiotica 46:225–233

    CAS  Article  Google Scholar 

  • Mahboub FA, Lamfon HA (2013) Protective effect of Gingo biloba extract on carbendazim-induced hepatotoxicity in albino rats. Food and Nutr Sci 4:866–872

    Article  Google Scholar 

  • Majdalawieh AF, Fayyad MW (2015) Immunomodulatory and anti-inflammatory action of Nigella sativa and thymoquinone: a comprehensive review. Int Immunopharmacol 28(1):295–304

    CAS  Article  Google Scholar 

  • María-Lourdes A, Mineko S, Margarita C, Angélica S, María-Isabe S, Víctor T, Fabiola-Gabriela Z, Ana-Rosa R (2012) Hepatic effects from subacute exposure to insecticides in adult male Wistar rats. Insecticides—Advances in Integrat Pest Manag:280–290

  • Mohamed WAM, Ismail SA, El-Hakim YMA (2014) Spirulina platensis ameliorative effect against GSM 900-MHz cellular phone radiation-induced genotoxicity in male Sprague-Dawley rats. Comp Clin Pathol 23(6):1719–1726

    Article  Google Scholar 

  • Mohamed WAM, Abd-Elhakim YMA, Farouk SM (2016) Protective effects of ethanolic extract of rosemary against lead-induced hepato-renal damage in rabbits. Exper and Toxicol Pathol 68(8):451–461

    CAS  Article  Google Scholar 

  • Mohammad HI, Iffat ZA, Mohammad TS (2015) Neuroprotective effects of Nigella sativa extracts during germination on central nervous system. Pharmaco Mag 11(1):S182–S189

    Google Scholar 

  • Mollazadeh H, Hosseinzadeh H (2014) The protective effect of Nigella sativa against liver injury: a review. Iran J Basic Med Sci 17(12):958–966

    Google Scholar 

  • Motawi TK, Teleb ZA, El-Boghdady NA, Ibrahim SA (2014) Effect of simvastatin and naringenin coadministration on rat liver DNA fragmentation and cytochrome P450 activity: an in vivo and in vitro study. J Physiol Biochem 70(1):225–237

    CAS  Article  Google Scholar 

  • Muthuviveganandavel V, Muthuraman P, Muthu S, Srikumar KJ (2008) Toxic effects of carbendazim at low dose levels in male rats. J of Toxicol Sci 33(1):33–30

    Article  Google Scholar 

  • Nada MA (2013) Protective effect of crude oil of Nigella sativa on liver in male albino mice treated with low toxic dose of paracetamol. Med J of Babylon 10(4):929–936

    Google Scholar 

  • Nishikimi M, Appaji N, Yagi K (1972) The occurrence of superoxide anion in the reaction of reduced phenazine methosulfate and molecular oxygen. Biochem Biophys Res Commun 46(2):849–854

    CAS  Article  Google Scholar 

  • Ogbera AO, Dada O, Adeyeye F, Jewo PI (2010) Complementary and alternative medicine use in diabetes mellitus. West Afr J Med 29(3):158–162

    CAS  Google Scholar 

  • Omonona AO, Jarikre TA (2015) Effect of carbendazim exposure and vitamin E supplementation in African Giant rats. J Agric Ecol Res Internat 4(1):1–9

    Google Scholar 

  • Pant N, Srivastava SP (2003) Testicular and spermatotoxic effects of quinalphos in rats. J Appl Toxicol 23(4):271–274

    CAS  Article  Google Scholar 

  • Patil JA, Patil AJ, Govindwar SP (2003) Biochemical effects of various pesticides on sprayers of grape gardens. Indian J Clin Biochem 18(2):16–22

    CAS  Article  Google Scholar 

  • Rabello-Gay MN, Ahmed AE (1980) Acrylonitrile: in vivo cytogenetic studies in mice and rats. Mutat Res 79:249–255

    CAS  Article  Google Scholar 

  • Rajeswary S, Kumaran B, Ilangovan R, Yuvaraj S, Sridhar M, Venkataraman P, Srinivasan N, Aruldhas MM (2007) Modulation of antioxidant defense system by the environmental fungicide carbendazim in Leydig cells of rats. Reprod Toxicol 24:371–380

    CAS  Article  Google Scholar 

  • Ramadan IA, Mohamed AA, Fahad IA (2014) Genoprotective and genotoxic effects of thymoquinone on doxorubicin-induced damage in isolated human leukocytes. Trop J Pharm Res 13(12):2015–2020

    Google Scholar 

  • Rašković A, Pavlović N, Kvrgić M, Jan S, Gorana M, Ivan Č, Momir M (2015) Effects of pharmaceutical formulations containing thyme on carbon tetrachlorideinduced liver injury in rats. BMC Complement Altern Med 15:442

  • Razia S, Siddiqui A (2015) Study on hepatic histopathological observations a sanctum on mancozeb induced toxicity in albino mice. Inter J of Global Sci Res 2(3):152–158

    Google Scholar 

  • Reisinger K, Szigeti J, Várnagy L (2006) Determination of carbendazim residues in the eggs, liver and pectoral muscle of Japanese quail (Coturnix coturnix japonica). Acta Vet Hung 54(1):127–133

    CAS  Article  Google Scholar 

  • Riad A, Adi G, Arik D, Shimon BS (2015) Anticancer activity of Nigella sativa (black seed) and its relationship with the thermal processing and quinone composition of the seed. Drug Des Devel Ther 9:3119–3124

    Google Scholar 

  • Saber TM, Reda MA (2016) Curcumin ameliorates mancozeb-induced neurotoxicity in rats. Japanese J of. Vet Res 64(2):S197–S202

    Google Scholar 

  • Sakr SA (2007) Ameliorative effect of ginger (Zingiber officinale) on mancozeb fungicide induced liver injury in albino rats. Aust J of Bas and Appl Sci 1(4):650–656

    CAS  Google Scholar 

  • Saleem U, Bashir A, Kanwal R , Saeed M , Maqsood A, Alia E (2012) Nephroprotective effect of vitamin C and Nigella sativa oil on gentamicin associated nephrotoxicity in rabbits. Pak J Pharm Sci 25(4):727–730

  • Salihu M, Ajayi BO, Adedara IA, Farombi EO (2016) 6-Gingerol-rich fraction from Zingiber officinale prevents hematotoxicity and oxidative damage in kidney and liver of rats exposed to carbendazim. J Diet Suppl 13(4):433–448

    CAS  Article  Google Scholar 

  • Salomi NJ, Nair SC, Jayawardhanan KK (1992) Antitumour principles from Nigella sativa seeds. Can Lett 63:41–46

    CAS  Article  Google Scholar 

  • Sharma E, Mustafa M, Pathak R, Guleria K, Ahmed RS, Vaid NB, Banerjee BD (2012) A case control study of gene environmental interaction in fetal growth restriction with special reference to organochlorine pesticides. Europ J of Obstet & Gynecol and Reprod Biol 161:163–169

    CAS  Article  Google Scholar 

  • Sherlock S (1981) Disease of the liver and biliary system, 8th edn. Blackwell Scientific Publication, Oxford

    Google Scholar 

  • Song JC, Deresinki S (2005) Hepatotoxicity of antifungal agents. Curr Opinion in Investig Drugs 6(2):170–177

    Google Scholar 

  • Srivastava AK, Ali W, Singh R, Bhui K, Tyagi S, AlKhedhairy AA, Srivastava PK, Musarrat J, Shukla Y (2012) Mancozeb-induced genotoxicity and apoptosis in cultured human lymphocytes. Life Sci 90:815–824

    CAS  Article  Google Scholar 

  • Srivastava AK, Sanjay M, Wahid A, Yogeshwer S (2016) Protective effects of lupeol against mancozeb-induced genotoxicity in cultured human lymphocytes. Phytomed 23:714–724

    CAS  Article  Google Scholar 

  • Sumanta C, Pratima B, Malay C, Prosun D, Jacintha AP, Samaresh C, Sujata L (2011) Pesticide induced alterations in marrow physiology and depletion of stem and stromal progenitor population: an experimental model to study the toxic effects of pesticide. Environm Toxicology:84–98

  • Suvarna KS, Layton C, Bancroft JD (2013) Bancroft’s theory and practice of histological techniques. Elsevier, U.K.

    Google Scholar 

  • Szepvolgyi J, Nagy K, Sajgone K, Regoly-Merci A, Soos K, Toth K, Pinter A, Antal M (1989) Subacute toxicological examination of Dithane M-45. Fd Chem Toxic 27(8):531–538

    CAS  Article  Google Scholar 

  • Tamhane A C, Dunlop D D (2000) Multiple comparisons of means. In Statistics and data analysis from elementary to intermediate 475–476

  • Tuzmen N, Candan N, Kaya E, Demiryas N (2008) Biochemical effects of chlorpyrifos and deltamethrin on altered antioxidative defense mechanisms and lipid peroxidation in rat liver. Cell Biochem Funct 26(1):119–124

    CAS  Article  Google Scholar 

  • Uchiyama M, Mihara M (1978) Determination of malondialdehyde precursor in tissues by thiobarbituric acid test. Anal Biochem 86:271–278

    CAS  Article  Google Scholar 

  • Uggini GK, Patel PV, Balakrishnan S (2012) Embryotoxic and teratogenic effects of pesticides in chick embryos: a comparative study using two commercial formulations. Environ Toxicol 27(3):166–174

    CAS  Article  Google Scholar 

  • Veerappan M, Pandurangan M, Suriyamurthy M, Srikumar K (2011) Acute toxicological evaluation of low dose methyl 2-benzimidazole carbamate fungicide on male albino rats. Iran J of Toxicology 4(4):381–389

    Google Scholar 

  • Worden J (2014) Antioxidant and oxidative stress and it molecular biochemical make up. McGraw Hill Press

  • Yahia E, Aiche MA, Chouabbia A (2014) Subchronic mancozeb treatment induced liver toxicity via oxidative stress in male Wistar rats. Commun Agric Appl Biol Sci 79:553–559

    CAS  Google Scholar 

  • Yahia E, Mohamed AA, Amel C, Mohamed SB (2015) Biochemical and hematological changes following long term exposure to mancozeb. Adv Biores 6(2):83–86

    CAS  Google Scholar 

  • Yesmin F, Rahman Z, Dewan JF, Helali AM, Rahman NIA, Alattraqchi AG, Ahmed A, Yousuf R, Salam A, Haque M (2013) Hepato-protective role of the aqueous and n-hexane extracts of Nigella sativa Linn in experimental liver damage in rats. Asian J of Pharm Clin Res 6(3):205–209

    Google Scholar 

  • Zaoui A, Cherrah Y, Lacaille-Dubois MA, Settaf A, Amarouch H, Hasar M (2000) Diuretic and and hypotensive effects of Nigella sativa in the spontaneously hypertensive rat. Therapie 55:379–382

    CAS  Google Scholar 

  • Zari TA, Al-Attar AM (2012) Therapeutic effects of olive leaves extract on rats treated with a sublethal concentration of carbendazim. Europ Rev Med Pharmac Sci 15(4):413–426

  • Zeba F, Mohammad A, Sana R, Aijaz A, Farah K (2016) Oral administration of Nigella sativa oil ameliorates the effect of cisplatin on membrane enzymes, carbohydrate metabolism and oxidative damage in rat liver. Toxicol Reports 3:328–335

    Article  Google Scholar 

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Acknowledgements

The authors wish to express their great thanks to Dr. Iman Elsayed El-Arabi, Associate Professor of Genetics and Genetic Engineering, Faculty of Veterinary Medicine, Zagazig University for her effort in performing the chromosomal aberrations technique and also Dr. Rehab El-Sayed Mowafy, Senior Researcher of Pathology, Zagazig Animal Health Research Institute for her help in histopathology.

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  1. Department of Clinical Pathology, Faculty of Veterinary Medicine, Zagazig University, Zagazig, Egypt

    Mohamed A. Hashem & Wafaa A. M. Mohamed

  2. Zagazig University Hospital, Faculty of Medicine, Zagazig University, Zagazig, Egypt

    Engy S. M. Attia

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  1. Mohamed A. Hashem
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Correspondence to Wafaa A. M. Mohamed.

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Hashem, M.A., Mohamed, W.A.M. & Attia, E.S.M. Assessment of protective potential of Nigella sativa oil against carbendazim- and/or mancozeb-induced hematotoxicity, hepatotoxicity, and genotoxicity. Environ Sci Pollut Res 25, 1270–1282 (2018). https://doi.org/10.1007/s11356-017-0542-9

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Keywords

  • Black seed oil
  • DNA fragmentation
  • Micronucleus
  • Genotoxicity
  • Fungicides
  • Oxidative stress