Environmental Science and Pollution Research

, Volume 25, Issue 30, pp 29910–29920 | Cite as

Biofilm formation is determinant in tomato rhizosphere colonization by Bacillus velezensis FZB42

  • Ameen Al-Ali
  • Jovana Deravel
  • François KrierEmail author
  • Max Béchet
  • Marc Ongena
  • Philippe Jacques
Chemistry, Activity and Impact of Plant Biocontrol products


In this work, the behavior in tomato rhizosphere of Bacillus velezensis FZB42 was analyzed taking into account the surfactin production, the use of tomato roots exudate as substrates, and the biofilm formation. B. velezensis FZB42 and B. amyloliquefaciens S499 have a similar capability to colonize tomato rhizosphere. Little difference in this colonization was observed with surfactin non producing B. velezensis FZB42 mutant strains. B. velezensis is able to grow in the presence of root exudate and used preferentially sucrose, maltose, glutamic, and malic acids as carbon sources. A mutant enable to produce exopolysaccharide (EPS-) was constructed to demonstrate the main importance of biofilm formation on rhizosphere colonization. This mutant had completely lost its ability to form biofilm whatever the substrate present in the culture medium and was unable to efficiently colonize tomato rhizosphere.


Rhizosphere Colonization Bacillus Lipopeptides Root exudates Biofilm 



The authors thank Dr. Rainer Borriss for kindly providing the Bacillus velezensis strains.

Funding information

This work was supported by the University of Lille 1 Sciences and Technologies, the European Funds of INTERREG IV PhytoBio Project and of INTERREG V Smartbiocontrol portfolio, BioProd project and the CPER FEDER project ALIBIOTECH. The authors thank the REALCAT platform for the use of BioLector in this work. The REALCAT platform is benefiting from a state subsidy administrated by the French National Research Agency (ANR) within the frame of the ‘Future Investments’ program (PIA), with the contractual reference ‘ANR-11-EQPX-0037’. The European Union, through the ERDF funding administered by the Hauts-de-France Region, has co-financed the platform. Centrale Lille, the CNRS, and Lille 1 University as well as the Centrale Initiatives Foundation, are thanked for their financial contributions to the acquisition and implementation of the equipment of the REALCAT platform. Ameen Al-Ali was a recipient of PhD scholarship awarded by Campus France through joint French-Iraqi governments program.


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Copyright information

© Springer-Verlag GmbH Germany 2017

Authors and Affiliations

  • Ameen Al-Ali
    • 1
    • 2
  • Jovana Deravel
    • 1
  • François Krier
    • 1
    Email author
  • Max Béchet
    • 1
  • Marc Ongena
    • 3
  • Philippe Jacques
    • 1
    • 3
  1. 1.Université Lille, INRA, ISA, Université Artois, Université Littoral Côte d’Opale, EA 7394-ICV Institut Charles ViolletteLilleFrance
  2. 2.College of Agriculture-Soil, Water and Environmental Research DepartmentAl-Qasim Green UniversityBabylonIraq
  3. 3.Terra Teaching and Research Centre, Microbial Processes and Interactions (MiPI)Gembloux Agro-Bio Tech, University of LiegeGemblouxBelgium

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