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Environmentally benign antifouling activity and toxic properties of bioactive metabolites from mangrove Excoecaria agallocha L

Abstract

This study was aimed to investigate the antifouling (AF) potentials and toxic properties of methanol extract from leaves of mangrove Excoecaria agallocha. Antimicrofouling activity results inferred that this extract strongly inhibited fouling bacterial and microalgal growth. This extract had also inhibited the settlement of brown mussel Perna indica and larvae of barnacle Balanus amphitrite. Further, EC50 < LC50 and therapeutic ratio > 1 together propagated non-toxic nature of the extract. Mollusk foot adherence assay result showed complete inhibition of foot spreading and loss of attachment of common rocky fouler Patella vulgata to the substrata. Field assay results affirmed that this extract effectively deterred settlement of biofoulers. Purification and GC-MS analysis of bioassay-guided active spot evidenced presence of three major compounds (> 85%) responsible for the promising AF activity. The identified lead compounds subjected to an estimation (BIOWIN™) program developed by United States Environmental Protection Agency (USEPA) predicts that they are biodegradable in nature.

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Acknowledgements

We gratefully acknowledge the financial assistance extended in the form of BSR-One time Grant by the UGC, New Delhi, India [F. No. 19-91/2013(BSR) dated October 2013].

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Correspondence to Ramasamy Ramasubburayan or Grasian Immanuel.

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Highlights

•Antifouling and toxic properties of leaves of mangrove Excoecaria agallocha L was studied.

•Methanol extract showed promising in vitro antimicro- and macrofouling activity.

•Toxicity assay using mussel and barnacle evidenced that the extract is non-toxic in nature.

•Field assay results inferred that extract effectively deterred settlement of fouling organisms.

•GC-MS analysis showed presence of antibiofouling compounds which are readily biodegradable.

Responsible editor: Philippe Garrigues

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Ramasubburayan, R., Prakash, S., Venkatesan, S. et al. Environmentally benign antifouling activity and toxic properties of bioactive metabolites from mangrove Excoecaria agallocha L. Environ Sci Pollut Res 24, 27490–27501 (2017). https://doi.org/10.1007/s11356-017-0297-3

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Keywords

  • Mangrove
  • Excoecaria agallocha
  • Antifouling
  • Field assay
  • GC-MS
  • Biodegradation