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Environmental Science and Pollution Research

, Volume 25, Issue 19, pp 18297–18302 | Cite as

Biological control of wilt disease complex on tomato crop caused by Meloidogyne javanica and Fusarium oxysporum f.sp. lycopersici by Verticillium leptobactrum

  • Lobna Hajji-HedfiEmail author
  • Hajer Regaieg
  • Asma Larayedh
  • Noura Chihani
  • Najet Horrigue-Raouani
Environmental sustainability and pollution prevention

Abstract

The efficacy of Verticillium leptobactrum isolate (HR1) was evaluated in the control of root-knot nematode and Fusarium wilt fungus under laboratory and greenhouse conditions. Five concentrations of V. leptobactrum (HR1) isolate were tested for their nematicidal and fungicidal activities against Meloidogyne javanica and Fusarium oxysporum f.sp. lycopersici in vitro. Laboratory trials showed that mycelium growth inhibition of Fusarium wilt fungus was correlated to the increase of the concentration of culture filtrate. All dilutions showed efficiency in reducing the growth of Fusarium oxysporum f.sp. lycopersici. The greatest nematicidal activity was observed at 50, 75, and 100% filtrate dilutions. The egg hatching percentage reached 42%, and the juvenile’s corrected mortality registered 90% for the above treatments. In greenhouse experiment, the biocontrol agent fungus enhanced significantly tomato growth components (height and weight of plant and root). The multiplication rate of root-knot nematode and the Fusarium wilt disease incidence declined significantly with soil application of V. leptobactrum as with chemical treatments. The isolate HR1 was efficient to control wilt disease complex caused by M. javanica and Fusarium oxysporum f.sp. lycopersici.

Keywords

Biocontrol Verticillium leptobactrum Root-knot nematode Fusarium wilt fungus Tomato 

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Copyright information

© Springer-Verlag GmbH Germany 2017

Authors and Affiliations

  • Lobna Hajji-Hedfi
    • 1
    Email author
  • Hajer Regaieg
    • 1
  • Asma Larayedh
    • 1
  • Noura Chihani
    • 1
  • Najet Horrigue-Raouani
    • 1
  1. 1.Higher Agronomic Institute of Chott-MariemSousse UniversitySousseTunisia

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