Evaluation of Beauveria bassiana infection in the hemolymph serum proteins of the housefly, Musca domestica L. (Diptera: Muscidae)
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Beauveria bassiana plays a prominent role in biocontrol of houseflies, Musca domestica (L.). Thus, a deeper insight into immune response of M. domestica during B. bassiana infection was warranted to assist the production of more efficient mycoinsecticides. The present study investigates changes in protein profile of M. domestica hemolymph serum post B. bassiana infection using two-dimensional difference gel electrophoresis (2D-DIGE) followed by identification of selected proteins by matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF MS). The non-infected or control group of flies showed an expression of 54 proteins, while M. domestica infected with B. bassiana expressed a total of 68 hemolymph serum proteins. Thirty three proteins were expressed in both groups of houseflies, whereas 35 proteins were exclusively expressed in infected flies and 21 proteins were exclusively expressed in control flies. Among the 33 proteins which were expressed in both groups of houseflies, 17 proteins showed downregulation, while16 proteins were upregulated in the infected flies compared to the non-infected ones. The results from this study are expected to facilitate better understanding of insect’s immune response mechanism.
KeywordsMusca domestica Entomopathogen Beauveria bassiana Hemolymph Immune response Protein expression
This paper is dedicated to the memory of Late, Padma Bhusahn, Dr. V. P. Sharma, Founder Director of National Institute of Malaria Research (India) and Former Additional Director General, Indian Council of Medical Research, who inspired the authors constantly with valuable suggestions on insect pest/vector control. Financial support by Indian Council of Medical Research (IRIS_ID No. 2010-07860), India and CSIR Research Associateship (09/086 (1191)/2014-EMR-I) to one of the author (SM) is gratefully acknowledged. The authors also acknowledge Mr. Satendar Singh (IIT Delhi, India) for his help in experimental work.
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