Antibiotic resistance genes in an urban river as impacted by bacterial community and physicochemical parameters
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Antibiotic resistance genes (ARGs) in urban rivers are a serious public health concern in regions with poorly planned, rapid development. To gain insights into the predominant factors affecting the fate of ARGs in a highly polluted urban river in eastern China, a total of 285 ARGs, microbial communities, and 20 physicochemical parameters were analyzed for 17 sites. A total of 258 unique ARGs were detected using high-throughput qPCR, and the absolute abundance of total ARGs was positively correlated with total organic carbon and total dissolved nitrogen concentrations (P < 0.01). ARG abundance and diversity were greatly altered by microbial community structure. Variation partitioning analysis showed that the combined effects of multiple factors contributed to the profile and dissemination of ARGs, and variation of microbial communities was the major factor affecting the distribution of ARGs. The disparate distribution of some bacteria, including Bacteroides from mammalian gastrointestinal flora, Burkholderia from zoonotic infectious diseases, and Zoogloea from wastewater treatment, indicates that the urban river was strongly influenced by point-source pollution. Results imply that microbial community shifts caused by changes in water quality may lead to the spread of ARGs, and point-source pollution in urban rivers requires greater attention to control the transfer of ARGs between environmental bacteria and pathogens.
KeywordsAntibiotic resistance genes Urban rivers Physicochemical parameters Microbial community structure High-throughput qPCR
This work was supported by the Natural Science Foundation of China (21677121 and 41571130064).
- Bogaerts P, Cuzon G, Evrard S, Hoebekeet M, Naas T, Glupczynski Y (2016) Evaluation of a DNA microarray for rapid detection of the most prevalent extended-spectrum β-lactamases, plasmid-mediated cephalosporinases and carbapenemases in Enterobacteriaceae, Pseudomonas and Acinetobacter. Int J Antmicrob Agents 48(2):189–193CrossRefGoogle Scholar
- Chen H, Zhang M (2013) Effects of advanced treatment systems on the removal of antibiotic resistance genes in wastewater treatment plants from Hangzhou, China. Environ Sci Technol 47:8157–8163Google Scholar
- Djordjevic SP, Stokes HW, Chowdhury PR (2013) Mobile elements, zoonotic pathogens and commensal bacteria: conduits for the delivery of resistance genes into humans, production animals and soil microbiota. Front Microbiol 4(86):1–12Google Scholar
- Garrity G, Brenner DJ, Kreig N, (2005) Bergey’s manual of systematic bacteriology, Vol. 2 Part C. The Alpha-, Beta-, Delta-, and Epsilonproteobacteria. Springer, New YorkGoogle Scholar
- Madigan MT, Martinko JM, Stahl D, Clark D (2012) Brock biology of microorganisms, 13th edn. Pearson/Benjamin Cummings, San Francisco, pp 368–381Google Scholar
- Nakayama T, Tuyet-Hoa TT, Harada K, Warisaya M, Asayama M, Hinenoya A, Lee WJ, Phu TM, Ueda S, Sumimura Y, Hirata K, Phuong NT, Yamamoto Y (2017) Water metagenomic analysis reveals low bacterial diversity and the presence of antimicrobial residues and resistance genes in a river containing wastewater from backyard aquacultures in the Mekong Delta, Vietnam. Environ Pollut 222:294–306CrossRefGoogle Scholar
- Rodriguez-Mozaz S, Chamorro S, Marti E, Martia E, Huertaa B, Grosa M, Sànchez-Melsióa A, Borregoa CM, Barcelóa D, Balcázar JL (2015) Occurrence of antibiotics and antibiotic resistance genes in hospital and urban wastewaters and their impact on the receiving river. Water Res 69:234–242CrossRefGoogle Scholar
- Statista (2016) Degree of urbanization by continent in 2016. http://www.statista.com/statistics/270860/ urbanization-by- continent/
- Woods DE, Sokol PA (2006) The genus Burkholderia. Springer, New York, pp 848–860Google Scholar
- Xiong W, Sun Y, Ding X, Wange M, Zeng Z (2015) Selective pressure of antibiotics on ARGs and bacterial communities in manure-polluted freshwater-sediment microcosms. Front Microbiol 6:1–8Google Scholar