Environmental Science and Pollution Research

, Volume 24, Issue 31, pp 24261–24271 | Cite as

Response of anaerobic membrane bioreactor to the presence of nano-Bi2WO6: reactor performance, supernatant characteristics, and microbial community

  • Lin ChenEmail author
  • Yue Wang
  • Chuqing Cao
  • Chang Liu
  • Liang Zhu
Research Article


Considering the increasing incorporation of manufactured nano-material into consumer products, there is a concern about its potential impacts in biological wastewater treatment. In this study, the response of anaerobic sludge to the presence of Bi2WO6 nano-particles (NPs) was investigated in the anaerobic membrane bioreactor (AnMBR). As the concentration of Bi2WO6 in the reactor was controlled around 1 mg/L, there was no significant difference in effluent water quality or bacterial activities before and after NP exposure, partially due to the microbial-induced NP aggregation and stable complex formation. However, with the increasing dosage of Bi2WO6 from 5 to 40 mg/L, great influences on the AnMBR performance were observed, including the reduction of COD removal efficiency, inhibition of the mechanization step, increased production of soluble microbial products, and enhanced secretion of extracellular polymer substrates. Additional investigation with high-throughput sequencing was conducted, clearly demonstrating that Bi2WO6 NPs induced changes in the bacterial community.


Anaerobic membrane bioreactor Bi2WO6 nano-particles COD removal Supernatant characteristics Microbial community 



This study was mainly financially supported by Natural Science Fund of Jiangsu (grant number BK20150813), National Natural Science Fund of China (grant number 51508153), Fundamental Research Funds for the Central Universities, and a project funded by the Priority Academic Program Development of Jiangsu Higher Education Institutions.

Supplementary material

11356_2017_22_MOESM1_ESM.docx (1.6 mb)
ESM 1 (DOCX 1615 kb)


  1. Amneklev J, Sörme L, Augustsson A, Bergbäck B (2015) The increase in bismuth consumption as reflected in sewage sludge. Water Air Soil Poll 226:92CrossRefGoogle Scholar
  2. Benndorf D, Loffhagen N, Babel W (2001) Protein synthesis patterns in Acinetobacter calcoaceticus induced by phenol and catechol show specificities of responses to chemostress. FEMS Microbiol Lett 200:247–252CrossRefGoogle Scholar
  3. Brown RE, Jarvis KL, Hyland KJ (1989) Protein measurement using bicinchoninic acid: elimination of interfering substances. Anal Biochem 180:136–139CrossRefGoogle Scholar
  4. Cammack WL, Kalff J, Prairie YT, Smith EM (2004) Fluorescent dissolved organic matter in lakes: relationships with heterotrophic metabolism. Limnol Oceanogr 49:2034–2045CrossRefGoogle Scholar
  5. Dollhopf S, Hashsham S, Dazzo F, Hickey R, Criddle C, Tiedje J (2001) The impact of fermentative organisms on carbon flow in methanogenic systems under constant low-substrate conditions. Appl Microbiol Biotechnol 56:531–538CrossRefGoogle Scholar
  6. Dubois M, Gilles KA, Hamilton JK, Rebers P, Smith F (1956) Colorimetric method for determination of sugars and related substances. Anal Chem 28:350–356CrossRefGoogle Scholar
  7. Fabrega J, Fawcett SR, Renshaw JC, Lead JR (2009) Silver nanoparticle impact on bacterial growth: effect of pH, concentration, and organic matter. Environ Sci Technol 43:7285–7290CrossRefGoogle Scholar
  8. Fang HHP, Xu L-C, Chan K-Y (2002) Effects of toxic metals and chemicals on biofilm and biocorrosion. Water Res 36:4709–4716CrossRefGoogle Scholar
  9. Fernández N, Díaz EE, Amils R, Sanz JL (2008) Analysis of microbial community during biofilm development in an anaerobic wastewater treatment reactor. Microb Ecol 56:121–132CrossRefGoogle Scholar
  10. Gómez-Rivera F, Field JA, Brown D, Sierra-Alvarez R (2012) Fate of cerium dioxide (CeO2) nanoparticles in municipal wastewater during activated sludge treatment. Bioresour Technol 108:300–304CrossRefGoogle Scholar
  11. Hou J, Miao L, Wang C, Wang P, Ao Y, Lv B (2015) Effect of CuO nanoparticles on the production and composition of extracellular polymeric substances and physicochemical stability of activated sludge flocs. Bioresour Technol 176:65–70CrossRefGoogle Scholar
  12. Laspidou CS, Rittmann BE (2002) A unified theory for extracellular polymeric substances, soluble microbial products, and active and inert biomass. Water Res 36:2711–2720CrossRefGoogle Scholar
  13. Li Y, Li A-M, Xu J, Li W-W, Yu H-Q (2013) Formation of soluble microbial products (SMP) by activated sludge at various salinities. Biodegradation 24:69–78CrossRefGoogle Scholar
  14. Li Y, Zhang C, Shuai D, Naraginti S, Wang D, Zhang W (2016a) Visible-light-driven photocatalytic inactivation of MS2 by metal-free g-C3N4: virucidal performance and mechanism. Water Res 106:249CrossRefGoogle Scholar
  15. Li Y, Chen L, Wang Y, Zhu L (2016b) Advanced nanostructured photocatalysts based on reduced graphene oxide-flower-like Bi2WO6 composites for an augmented simulated solar photoactivity activity. Mater Sci Eng 210:29–36CrossRefGoogle Scholar
  16. Marshall CW, Ross DE, Fichot EB, Norman RS, May HD (2012) Electrosynthesis of commodity chemicals by an autotrophic microbial community. Appl Environ Microb 78:8412–8420CrossRefGoogle Scholar
  17. Mei X, Wang Z, Zheng X, Huang F, Ma J, Tang J, Wu Z (2014) Soluble microbial products in membrane bioreactors in the presence of ZnO nanoparticles. J Membrane Sci 451:169–176CrossRefGoogle Scholar
  18. Morgan J, Forster C, Evison L (1990) A comparative study of the nature of biopolymers extracted from anaerobic and activated sludges. Water Res 24:743–750CrossRefGoogle Scholar
  19. Otero-González L, Field JA, Sierra-Alvarez R (2014a) Fate and long-term inhibitory impact of ZnO nanoparticles during high-rate anaerobic wastewater treatment. J Environ Manag 135:110–117CrossRefGoogle Scholar
  20. Otero-González L, Field JA, Sierra-Alvarez R (2014b) Inhibition of anaerobic wastewater treatment after long-term exposure to low levels of CuO nanoparticles. Water Res 58:160–168CrossRefGoogle Scholar
  21. Puay N-Q, Qiu G, Ting Y-P (2015) Effect of zinc oxide nanoparticles on biological wastewater treatment in a sequencing batch reactor. J Clean Prod 88:139–145CrossRefGoogle Scholar
  22. Rahman L, Corns WT, Bryce DW, Stockwell PB (2000) Determination of mercury, selenium, bismuth, arsenic and antimony in human hair by microwave digestion atomic fluorescence spectrometry. Talanta 52:833–843CrossRefGoogle Scholar
  23. Rice EW, Bridgewater L, Association APH (2012) Standard methods for the examination of water and wastewater, American Public Health Association Washington, DCGoogle Scholar
  24. Romero-Güiza MS, Vila J, Mata-Alvarez J, Chimenos JM, Astals S (2016) The role of additives on anaerobic digestion: a review. Renew Sust Energ Rev 58:1486–1499CrossRefGoogle Scholar
  25. Shen P, Zhang J, Zhang J, Jiang C, Tang X, Li J, Zhang M, Wu B (2013) Changes in microbial community structure in two anaerobic systems to treat bagasse spraying wastewater with and without addition of molasses alcohol wastewater. Bioresour Technol 131:333–340CrossRefGoogle Scholar
  26. Sheng Z, Liu Y (2011) Effects of silver nanoparticles on wastewater biofilms. Water Res 45:6039–6050CrossRefGoogle Scholar
  27. Sheng G-P, Yu H-Q, Li X-Y (2010) Extracellular polymeric substances (EPS) of microbial aggregates in biological wastewater treatment systems: a review. Biotechnol Adv 28:882–894CrossRefGoogle Scholar
  28. Smith AL, Stadler LB, Love NG, Skerlos SJ, Raskin L (2012) Perspectives on anaerobic membrane bioreactor treatment of domestic wastewater: a critical review. Bioresour Technol 122:149–159CrossRefGoogle Scholar
  29. Tan M, Qiu G, Ting YP (2015) Effects of ZnO nanoparticles on wastewater treatment and their removal behavior in a membrane bioreactor. Bioresour Technol 185:125–133CrossRefGoogle Scholar
  30. Tian Y, Chen L, Jiang T (2011) Characterization and modeling of the soluble microbial products in membrane bioreactor. Sep Purif Technol 76:316–324CrossRefGoogle Scholar
  31. Wang X-H, Gai L-H, Sun X-F, Xie H-J, Gao M-M, Wang S-G (2010) Effects of long-term addition of Cu(II) and Ni(II) on the biochemical properties of aerobic granules in sequencing batch reactors. Appl Microbiol Biotechnol 86:1967–1975CrossRefGoogle Scholar
  32. Wang B-B, Peng D-C, Hou Y-P, Li H-J, Pei L-Y, Yu L-F (2014) The important implications of particulate substrate in determining the physicochemical characteristics of extracellular polymeric substances (EPS) in activated sludge. Water Res 58:1–8CrossRefGoogle Scholar
  33. Yang Y, Quensen J, Mathieu J, Wang Q, Wang J, Li M, Tiedje JM, Alvarez PJJ (2014) Pyrosequencing reveals higher impact of silver nanoparticles than Ag+ on the microbial community structure of activated sludge. Water Res 48:317–325CrossRefGoogle Scholar
  34. Yao S, Wei J, Huang B, Feng S, Zhang X, Qin X, Wang P, Wang Z, Zhang Q, Jing X, Zhan J (2009) Morphology modulated growth of bismuth tungsten oxide nanocrystals. J Solid State Chem 182:236–239CrossRefGoogle Scholar
  35. Zhang C, Liang Z, Hu Z (2014) Bacterial response to a continuous long-term exposure of silver nanoparticles at sub-ppm silver concentrations in a membrane bioreactor activated sludge system. Water Res 50:350–358CrossRefGoogle Scholar
  36. Zhang C, Li Y, Wang D, Zhang W, Wang Q, Wang Y, Wang P (2015) Ag@helical chiral TiO2 nanofibers for visible light photocatalytic degradation of 17α-ethinylestradiol. Environ Sci Pollut R 22:10444–10451CrossRefGoogle Scholar
  37. Zhang C, Li Y, Wang C, Niu L, Cai W (2016a) Occurrence of endocrine disrupting compounds in aqueous environment and their bacterial degradation: a review. Crit Rev Environ Sci Technol 46:1–59CrossRefGoogle Scholar
  38. Zhang J, Dong Q, Liu Y, Zhou X, Shi H (2016b) Response to shock load of engineered nanoparticles in an activated sludge treatment system: insight into microbial community succession. Chemosphere 144:1837–1844CrossRefGoogle Scholar
  39. Zheng X, Chen Y, Wu R (2011) Long-term effects of titanium dioxide nanoparticles on nitrogen and phosphorus removal from wastewater and bacterial community shift in activated sludge. Environ Sci Technol 45:7284–7290CrossRefGoogle Scholar
  40. Zhou L, Zhang Z, Xia S, Jiang W, Ye B, Xu X, Gu Z, Guo W, Ngo H-H, Meng X, Fan J, Zhao J (2014) Effects of suspended titanium dioxide nanoparticles on cake layer formation in submerged membrane bioreactor. Bioresour Technol 152:101–106CrossRefGoogle Scholar
  41. Zhu S, Xu T, Fu H, Zhao J, Zhu Y (2007) Synergetic effect of Bi2WO6 photocatalyst with C60 and enhanced photoactivity under visible irradiation. Environ Sci Technol 41:6234CrossRefGoogle Scholar

Copyright information

© Springer-Verlag GmbH Germany 2017

Authors and Affiliations

  • Lin Chen
    • 1
    • 2
    Email author
  • Yue Wang
    • 1
    • 2
  • Chuqing Cao
    • 3
  • Chang Liu
    • 1
    • 2
  • Liang Zhu
    • 1
    • 2
  1. 1.Key Laboratory of Integrated Regulation and Resources Development of Shallow LakesHohai UniversityNanjingChina
  2. 2.College of EnvironmentHohai UniversityNanjingChina
  3. 3.School of Mechanical EngineeringNangjing University of Technology and ScienceNanjingChina

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