Individual and combined effect of chlorpyrifos and cypermethrin on reproductive system of adult male albino rats
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Commercial mixtures of chlorpyrifos and cypermethrin pesticides are widely used to enhance the toxic effects of cypermethrin on target insects. So, the purpose of the current study was to evaluate the individual and combined toxic effects of chlorpyrifos (CPF) and cypermethrin (CYP) on reproductive system of adult male albino rats. Forty adult male albino rats were randomized into main four groups: group I (control group) included 16 rats, subdivided into negative and positive control; group II (eight rats) received chlorpyrifos 6.75 mg/kg b.w./orally∕daily); group III (eight rats) (received cypermethrin 12.5 mg/kg b.w./orally∕daily); and group IV (eight rats) (received chlorpyrifos and cypermethrin at the same previously mentioned doses). All treatments were given by oral gavage for 12 weeks. We found that single CPF and CYP exposures significantly have adverse effects on reproductive function of adult male albino rats manifested by reduced testicular weight, decreased sperm count, motility and viability, significantly increased percent of morphologically abnormal spermatozoa, and significant increments in sperm DNA fragmentation index (DFI) with respect to control group. Furthermore, serum follicle stimulating hormone, luteinizing hormone, and testosterone levels were decreased significantly compared to control group. This was accompanied with histopathological changes in the testis of rats such as necrosis, degeneration, decreasing number of spermatogenic cells in some seminiferous tubules, edema, congested blood vessels, and exudate in interstitial tissue of the testis. Notably, all these changes were exaggerated in rats treated concomitantly with chlorpyrifos and cypermethrin rendering the mixture more toxic than the additive effects of each compound and causing greater damage on the reproductive system of male albino rats than the individual pesticides.
KeywordsPesticides Organophosphorus Pyrethroids Chlorpyrifos Cypermethrin Reproductive toxicity
Sincere appreciation is expressed to Prof./Dr. Mai Samir for her valuable guidance and to all members in the Forensic Medicine and Clinical Toxicology Department for their encouragement.
Compliance with ethical standards
The experiment was carried out in compliance with the “Guide of the Care and Use of Laboratory Animals” (Clark et al., 1996) and the institutional guidelines for the care and use of experimental animals approved by the Medical Research Ethics Committee of Zagazig University, Egypt.
Conflict of interest
The authors declare that they have no conflict of interest.
There is no financial support for the research, authorship, and/or publication of this article.
- Ahmad L, Khan A, Khan MZ (2012) Pyrethroid-induced reproductive toxicopathology in non-target species. Pak Vet J 32(1):1–9Google Scholar
- Akhtar N, Srivastava MK, Raizada RB (2009) Assessment of chlorpyrifos toxicity on certain organs in rat, Rattus norvegicus. J Environ Biol 30(6):1047–1053Google Scholar
- Assayed M, Salem H, Khalaf A et al (2008) Protective effects of garlic extract and vitamin C against cypermethrin reproductive toxicity in male rats. J Vet Sci 1(1):1–15Google Scholar
- Bancroft JD, Gamble M (2007) Connective tissue stains. In: Bancroft JD, Gamble M (eds) Theory and practice of histological techniques. 6th ed. Churchill Livingstone, London New York Philadelphia, p. 150Google Scholar
- Chen L, Wang R, Wang W, Lu W, Xiao Y, Wang D, Dong Z (2015) Hormone inhibition during mini-puberty and testicular function in male rats. International journal of endocrinology and metabolism 13(4)Google Scholar
- Choudhary N, Goyal R, Joshi SC (2008) Effect of malathion on reproductive system of male rats. J Environ Biol 29:259–262Google Scholar
- Clark JD, Baldwin RL, Bayne KA, Brown M, Gebhart GF, Gonder JC, Gwathmey JK, Keeling ME, Kohn DF, Robb JW, Smith OA (1996) Guide for the care and use of laboratory animals. Institute of Laboratory Animal Resources, National Research Council. Washington, DC, p 125Google Scholar
- Goel A, Dani V, Dhawan D K (2007) Zinc mediates normalization of hepatic drug metabolizing enzymes in chlorpyrifos-induced toxicity. Toxicol Letters 169:26–33Google Scholar
- Greene S, Pohanish R (2005) Sittig’s handbook of pesticides and agricultural chemicals. William Andrew, Norwich, NY, pp. 174–177Google Scholar
- Hussien H, Abdoub H, Yousef M et al (2011) Cypermethrin induced damage in genomic DNA and histopathological changes in brain and haematotoxicity in rats: the protective effect of sesame oil. Brain Res Bull 87(5):402–419Google Scholar
- Idris SB, Ambali SF, Ayo JO (2012) Cytotoxicity of chlopyrifos and cypermethrin: the ameliorative effects of antioxidants. Afr J Biotechnol 11(99):16461–16467Google Scholar
- Ikpeme EV, Udensi O, Ekaluo UB, Uyoh EA, Asuquo BO, Udoh FV, Udoh PB (2007) Effect of crude extract of Caricapapaja seeds on the reproductive efficiency of male albino rats. Global J Pure Applied Sci 13:365–368Google Scholar
- LI YF, Chen PA, HU JX, Jing LI, XU LC (2013) Effects of cypermethrin on male reproductive system in adult rats. Biomed Environ Sci 26(3):201–208Google Scholar
- Manna S, Bhattacharyya D, Mandal T et al (2004) Repeated dose toxicity of alfa cypermethrin in rats. J Vet Sci 5:241–245Google Scholar
- Mori K, Kaido M, Fujishiro K et al (1991) Dose dependent effects of inhaled ethylene oxide on spermatogenesis in rats. Br J Ind Med 48(4):270–274Google Scholar
- Noaishi MA, Allah AA, Afify MM (2013) Oral and dermal exposure of chlorpyrifos and cypermethrin mixture induced cytogenetic, histopathological damage and oxidative stress in rats. J Am Sci 9:56–65Google Scholar
- SPSS Inc. (2007) SPSS for Windows, Version 16.0. Chicago, SPSS Inc. available from: http://www.unimuenster.de/imperia/md/content/ziv/service/software/spss/handbuecher/englisch/spss_brief_guide_16.0.pdf.
- World Health Organization (WHO) (1996) The world health report: fighting disease, fostering development. WHO, GenevaGoogle Scholar
- Wielgomas B, Krechniak J (2007) Toxicokinetic interactions of alpha-cypermethrin and chlorpyrifos in rats. Pol J Environ Stud 16(2):267Google Scholar