Environmental Science and Pollution Research

, Volume 24, Issue 1, pp 813–826 | Cite as

Upgrading the hydrolytic potential of immobilized bacterial pretreatment to boost biogas production

  • U. Ushani
  • S. Kavitha
  • M. Johnson
  • Ick Tae Yeom
  • J. Rajesh Banu
Research Article


In this study, surfactant dioctyl sodium sulphosuccinate (DOSS)-mediated immobilized bacterial pretreatment of waste activated sludge (WAS) was experimentally proved to be an efficient and economically feasible process for enhancing the biodegradability of WAS. The maximal floc disruption with negligible cell cleavage was achieved at surfactant dosage of 0.009 g/g SS. Results of the outcome of bacterial pretreatment of sludge biomass revealed that chemical oxygen demand (COD) solubilization for deflocculated (EPS removed—bacterially pretreated) sludge was 20 %, which was higher than that of flocculated (14 %) or control (5 %). The pretreatment was swift in deflocculated sludge with a rate constant of about 0.064 h−1. Biochemical methane potential (BMP) assay resulted in significant methane yield at 0.24 gCOD/gCOD for deflocculated sludge. Economic assessment of the proposed method showed a net profit of about 57.39 USD/ton of sludge.


Waste activated sludge Dioctyl sodium sulphosuccinate Immobilization Protease enzyme Methane 


  1. APHA (American Public Health Association) (2005) Standard methods for the examination of water and wastewater, 21st edn. American water works association (Water Environment Federation), Washington, DC, USAGoogle Scholar
  2. Appels L, Baeyens J, Degreve J, Dewil R (2008) Principles and potential of the anaerobic digestion of waste-activated sludge. Prog Energy Combust Sci 34:755–781CrossRefGoogle Scholar
  3. Appels L, Houtmeyers S, Degreve J, Van Impe J, Dewil R (2013) Influence of microwave pre-treatment on sludge solubilization and pilot scale semicontinuous anaerobic digestion. Bioresour Technol 128:598CrossRefGoogle Scholar
  4. Azize A (2005) Enzymatic treatment effects on dewaterability of anaerobically digested biosolids-I: performance evaluations. Process Biochem 40:2427–2434CrossRefGoogle Scholar
  5. Batstone DJ, Tait S, Starrenburg D (2009) Estimation of hydrolysis parameters in full-scale anaerobic digesters. Biotechnol Bioeng 102:1513–1520CrossRefGoogle Scholar
  6. Bazot S, Lebeau T (2009) Effect of immobilization of a bacterial consortium on diuron dissipation and community dynamics. Bioresour Technol 100:4257–4261CrossRefGoogle Scholar
  7. Bhuyan AK (2010) On the mechanism of SDS-induced protein denaturation. Biopolymers 93:186–199CrossRefGoogle Scholar
  8. Cadoret A, Conrad A, Block JC (2002) Availability of low and high molecular weight substrates to extracellular enzymes in whole and dispersed activated sludges. Enzym Microb Technol 31:179–186CrossRefGoogle Scholar
  9. Carrère H, Dumas C, Battimelli A, Batstone DJ, Delgenès JP, Steyer JP, Ferrer I (2010) Pretreatment methods to improve sludge anaerobic degradability: a review. J Hazard Mater 183:1–15CrossRefGoogle Scholar
  10. Cesaro A, Belgiorno V (2014) Pretreatment methods to improve anaerobic biodegradability of organic municipal solid waste fractions. Chem Eng J 240:24–37CrossRefGoogle Scholar
  11. Dhar BR, Nakhla G, Ray MB (2012) Techno-economic evaluation of ultrasound and thermal pretreatments for enhanced anaerobic digestion of municipal waste activated sludge. Waste Manag 32:542–549CrossRefGoogle Scholar
  12. Emmanuel N, Courtney AO, Matthew JD, Ram BG (2014) Comparison of the effectiveness of solid and solubilized dioctyl sodium sulfosuccinate (DOSS) on oil dispersion using the baffled flask test, for crude oil spill applications. Ind Eng Chem Res 53:11862–11872CrossRefGoogle Scholar
  13. Feng LY, Yan YY, Chen YG (2009) Kinetic analysis of waste activated sludge hydrolysis and short-chain fatty acids production at pH 10. J Environ Sci Chin 21(5):589–594CrossRefGoogle Scholar
  14. Foladori P, Tamburini S, Bruni L (2010) Bacteria permeabilisation and disruption caused by sludge reduction technologies evaluated by flow cytometry. Water Res 44:4888–4899CrossRefGoogle Scholar
  15. Gayathri T, Kavitha S, Adish Kumar S, Kaliappan S, Yeom IT, Rajesh BJ (2015) Effect of citric acid induced deflocculation on the ultrasonic pretreatment efficiency of dairy waste activated sludge. Ultrason Sonochem 22:333–340CrossRefGoogle Scholar
  16. Guangyin Z, Xueqin L, You LY, Youcai Z (2014) Combined electrical-alkali pretreatment to increase the anaerobic hydrolysis rate of waste activated sludge during anaerobic digestion. Appl Energy 128:93–102CrossRefGoogle Scholar
  17. Gyu YH, Yeun CH, Daewon P (2014) Improvement of sludge anaerobic degradability by combined electro-flotation and electro-oxidation treatment. Biochem Eng J 90:44–48CrossRefGoogle Scholar
  18. Hamid-reza KH, Kohzo K, Fumio K (2003) Wastewater treatment with bacteria immobilized onto a ceramic carrier in an aerated system. J Biosci Bioeng 95:128–132CrossRefGoogle Scholar
  19. Hou J, Miao L, Wang C, Wang P, Ao Y, Qian J, Dai S (2014) Inhibitory effects of ZnO nanoparticles on aerobic wastewater biofilms from oxygen concentration profiles determined by microelectrodes. J Hazard Mater 276:164–170CrossRefGoogle Scholar
  20. Houtmeyers S, Degrève J, Willems K, Dewil R, Appels L (2014) Comparing the influence of low power ultrasonic and microwave pre-treatments on the solubilisation and semi-continuous anaerobic digestion of waste activated sludge. Bioresour Technol 171:44–49CrossRefGoogle Scholar
  21. Jang JH, Ahn JH (2013) Effect of microwave pretreatment in presence of NaOH on mesophilic anaerobic digestion of thickened waste activated sludge. Bioresour Technol 131:437–442CrossRefGoogle Scholar
  22. Jensen PD, Ge H, Batstone DJ (2011) Assessing the role of biochemical methane potential tests in determining anaerobic degradability rate and extent. Water Sci Technol 64:880–886CrossRefGoogle Scholar
  23. Junjie TAO, Shoulin WU, Linbo SUN, Xiaobo TAN, Shimiao YU, Zhibin ZHANG (2012) Composition of waste sludge from municipal wastewater treatment plant. Procedia Environ Sci 12:964–971CrossRefGoogle Scholar
  24. Kavitha S, Adish Kumar S, Yogalakshmi KN, Rajesh BJ (2013) Effect of enzyme secreting bacterial pretreatment on enhancement of aerobic digestion potential of waste activated sludge interceded through EDTA. Bioresour Technol 150:210–219CrossRefGoogle Scholar
  25. Kavitha S, Adish Kumar S, Kaliappan S, Yeom IT, Rajesh BJ (2014a) Improving the amenability of municipal waste activated sludge for biological pretreatment by phase-separated sludge disintegration method. Bioresour Technol 169:700–706CrossRefGoogle Scholar
  26. Kavitha S, Jayashree C, Adish Kumar S, Yeom IT, Rajesh BJ (2014b) The enhancement of anaerobic biodegradability of waste activated sludge by surfactant mediated biological pretreatment. Bioresour Technol 168:159–166CrossRefGoogle Scholar
  27. Kavitha S, Adish Kumar S, Kaliappan S, Ick TY, Rajesh BJ (2015a) Achieving profitable biological sludge disintegration through phase separation and predicting its anaerobic biodegradability by non- linear regression model. Chem Eng J 279:478–487CrossRefGoogle Scholar
  28. Kavitha S, Kaliappan S, Adish Kumar S, Yeom IT, Rajesh BJ (2015b) Effect of NaCl induced floc disruption on biological disintegration of sludge for enhanced biogas production. Bioresour Technol 192:807–811CrossRefGoogle Scholar
  29. Kavitha S, Saranya T, Kaliappan S, Adish Kumar S, Yeom IT, Rajesh BJ (2015c) Accelerating the sludge disintegration potential of a novel bacterial strain Planococcus jake 01 by CaCl2 induced deflocculation. Bioresour Technol 175:396–405CrossRefGoogle Scholar
  30. Kavitha S, Yukesh Kannah R, Yeom Ick T, Do KU, Rajesh BJ (2015d) Combined thermo-chemo-sonic disintegration of waste activated sludge for biogas production. Bioresour Technol 197:383–392CrossRefGoogle Scholar
  31. Kavitha S, Rajesh BJ, Vinoth Kumar J, Rajkumar M (2016a) Improving the biogas production performance of municipal waste activated sludge via disperser induced microwave disintegration Bioresour. Technol 217:21–27Google Scholar
  32. Kavitha S, Saji Pray S, Yogalakshmi KN, Adish Kumar S, Ick-Tae Y, Rajesh BJ (2016b) Effect of chemo-mechanical disintegration on sludge anaerobic digestion for enhanced biogas production. Environ Sci Pollut Res 23:2402–2414CrossRefGoogle Scholar
  33. Keffala C, Harerimana C, Vasel J (2013) A review of the sustainable value and disposal techniques, wastewater stabilisation ponds sludge characteristics and accumulation. Environ Monitoring and Assessment 185(1):45–58CrossRefGoogle Scholar
  34. Libing C, Sangtian Y, Xin Hui X, Xulin S, Benjamin J (2009) Progress and perspectives of sludge ozonation as a powerful pretreatment method for minimization of excess sludge production. Water Res 43:1811–1822CrossRefGoogle Scholar
  35. Luo K, Yang Q, Yu J, Li X, Yang G, Xie B, Yang F, Zheng W, Zeng G (2011) Combined effect of sodium dodecyl sulfate and enzyme on waste activated sludge hydrolysis and acidification. Bioresou Technol 102:7103–7110CrossRefGoogle Scholar
  36. Luo K, Yang Q, Li XM, Yang G, Liu Y, Wang DB, Zheng W, Zeng GM (2012) Hydrolysis kinetics in anaerobic digestion of waste activated sludge enhanced by a amylase. J Biochem Eng 62:17–21CrossRefGoogle Scholar
  37. Merrylin J, Kaliappan S, Adish Kumar S, Yeom IT, Rajesh BJ (2013) Effect of extracellular polymeric substances on sludge reduction potential of Bacillus licheniformis. Int J Environ Sci Technol 10:85–92CrossRefGoogle Scholar
  38. Motevasel M (2014) A study of surface biosurfactants applications on oil degradation. American J Oil Chem Technol 2:9Google Scholar
  39. Metcalf and Eddy (2003) Wastewater engineering treatment and reuse, fourth ed. McGraw Hill publication, New York, USAGoogle Scholar
  40. Neyens E, Baeyens J, Dewil R, De heyder B (2004) Advanced sludge treatment affects extracellular polymeric substances to improve activated sludge dewatering. J Hazard Mater 106:83–92CrossRefGoogle Scholar
  41. Paliwal R, Uniyal S, Rai JP (2015) Evaluating the potential of immobilized bacterial consortium for black liquor biodegradation. Environ Sci Pollut Res Int 22(9):6842–6853CrossRefGoogle Scholar
  42. Parmar N, Singh A, Ward OP (2001) Characterization of the combined effects of enzyme, pH and temperature treatments for removal of pathogens from sewage sludge. Biotechnol Lett 17:169–172Google Scholar
  43. Passos F, Ferrer I (2014) Microalgae conversion to biogas: thermal pretreatment contribution on net energy production. Environ Sci Technol 48:7171–7178CrossRefGoogle Scholar
  44. Roman HJ, Burgess JE, Pletschke BI (2006) Enzyme treatment to decrease solids and improve digestion of primary sewage sludge. African J Biotech 5:963–967Google Scholar
  45. Saha M, Eskicioglu C, Marin J (2011) Microwave, ultrasonic and chemomechanical pretreatments for enhancing methane potential of pulp mill wastewater treatment sludge. Bioresour Technol 102:7815–7826CrossRefGoogle Scholar
  46. Sahinkaya S, Mehmet Faik S (2013) Sono-thermal pre-treatment of waste activated sludge before anaerobic digestion. Ultrasonic Sonochem 20:587–594CrossRefGoogle Scholar
  47. Simões M, Simões Lu´ cia C, Vieira Maria J (2010) A review of current and emergent biofilm control strategies. LWT-Food Sci Technol 43:573–583CrossRefGoogle Scholar
  48. Simón M, García I, González V, Romero A, Martín F (2014) Effect of grain size and heavy metals on As immobilization by marble particles. Environ Sci Pollut ResGoogle Scholar
  49. Sombatsompop K, Visvanathan C, Ben Aim R (2006) Evaluation of biofouling phenomenon in suspended and attached growth membrane bioreactor systems. Desalination 201(1–3):138–149CrossRefGoogle Scholar
  50. Sutherland I (2001) Biofilm exopolysaccharides: a strong and sticky framework. Microbiology 147(1):3–9CrossRefGoogle Scholar
  51. Uma R, Adish KS, Kaliappan S, Ick TY, Rajesh BJ (2012) Low temperature thermo-chemical pretreatment of dairy waste activated sludge for anaerobic digestion process. Bioresour Technol 103:415–424CrossRefGoogle Scholar
  52. Uma R, Adish KS, Kaliappan S, Ick TY, Rajesh BJ (2013) Impacts of microwave pretreatments on the semi-continuous anaerobic digestion of dairy waste activated sludge. Waste Manag 33:1119–1127CrossRefGoogle Scholar
  53. Veera Lakshmi M, Merrylin J, Kavitha S, Adish Kumar S, Rajesh BJ, Ick-Tae Y (2014) Solubilization of municipal sewage waste activated sludge by novel lytic bacterial strains. Environ Sci Pollut Res 21:2733–2743CrossRefGoogle Scholar
  54. Vlyssides AG, Karlis PK (2004) Thermal–alkaline solubilization of waste activated sludge as a pre-treatment stage for anaerobic digestion. Bioresour Technol 91:201–206CrossRefGoogle Scholar
  55. Whitman WB, Goodfellow M, Kämpfer P, Busse HJ, Trujillo ME, Ludwig W, Suzuki K (eds) (2012) Bergey’s manual of systematic bacteriology, 2nd edn., vol. 5, parts A and B. Springer-Verlag, New York, NYGoogle Scholar
  56. Wilen BM, Lumleyb D, Mattssonb A, Minoc T (2008) Relationship between floc composition and flocculation and settling properties studied at a full scale activated sludge plant. Water Res 4(2):4404–4418CrossRefGoogle Scholar
  57. Xiaocong L, Huan L, Yuyao Z, Can L, Qingwu C (2016) Accelerated high-solids anaerobic digestion of sewage sludge using low-temperature thermal pretreatment. Int Biodeterior Biodegrad 106:141–149Google Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 2016

Authors and Affiliations

  • U. Ushani
    • 1
  • S. Kavitha
    • 1
  • M. Johnson
    • 2
  • Ick Tae Yeom
    • 3
  • J. Rajesh Banu
    • 1
  1. 1.Department of Civil EngineeringRegional Centre of Anna UniversityTirunelveliIndia
  2. 2.St. Xavier CollegeTirunelveliIndia
  3. 3.Department of Civil and Environment EngineeringSungkyunkwan UniversitySeoulSouth Korea

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