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Hepatoprotective and nephroprotective effects of sardinelle (Sardinella aurita) protein hydrolysate against ethanol-induced oxidative stress in rats

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Abstract

Ethanol consumption-induced oxidative stress that is a major etiological factor has been proven to play important roles in organs’ injury. In the present study, we investigated the protective effect of fish protein hydrolysate prepared from the heads and viscera of sardinelle (Sardinella aurita) (SPH) against the toxicity of ethanol on the liver and kidney of adult male rats. Animals were divided into four groups of six animals each: group C served as control, group Eth received 30 % ethanol solution at the dose of 3 g/kg body weight, group SPH received only 7.27 mg of SPH/kg body weight, and group Eth-SPH received ethanol and SPH simultaneously at the doses of 30 % and 7.27 mg/kg body weight, respectively. All groups were treated by gavage way for 15 days. Ethanol treatment decreased the defense enzymatic system including superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPx), which increased after the co-administration of SPH. Malondialdehyde (MDA) and toxicity biomarker levels such as aspartate transaminase (AST) and alanine transaminase (ALT) and alcaline phosphatase (ALP) and gamma-glutamyl transaminase (GGT) activities were enhanced after chronic ethanol treatment and reduced by co-treatment with SPH. The histological examination of the liver and kidney confirmed biochemical changes in ethanol-treated rats and demonstrated the protective role of SPH.

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References

  • Adedara IA, Owumi SE, Uwaifo AO, Farombi EO (2010) Aflatoxin B1 and ethanol co-exposure induces hepatic oxidative damage in mice. Toxicol Ind Health 26(10):717–724

    Article  CAS  Google Scholar 

  • Aebi H (1984) Catalase in vitro. Meth Enzymol 105:121–126

    Article  CAS  Google Scholar 

  • Agarwal DP, Goedde HW (1990) Alcohol metabolism, alcohol intolerance and alcoholism: biochemical and pharmacogenetic approaches. Springer, New York

    Book  Google Scholar 

  • Albano E (2006) Alcohol, oxidative stress and free radical damage. Proc Nutr Soc 65(3):278–290

    Article  CAS  Google Scholar 

  • Amarowicz R, Shahidi F (1997) Antioxidant activity of peptide fractions of capelin protein hydrolysates. Food Chem 58:355–359

    Article  CAS  Google Scholar 

  • Ambadath V, Gopal VR, Madambath I (2010) Comparative study of the efficacy of ascorbic acid, Quercetin, and thiamine for reversing ethanol-induced toxicity. J Med Food 13(6):1485–1489

    Article  CAS  Google Scholar 

  • Atessahin A, Yilmaz S, Karahan I, Ceribasi AO, Karaoglu A (2005) Effets du lycopène contre la néphrotoxicité induite par le cisplatine et le stress oxydatif chez les rats. Toxicologie 212(2–3):116–123

    Article  CAS  Google Scholar 

  • Balasubramaniyan V, Sailaja JK, Nalini N (2003) Role of leptin on alcohol-induced oxidative stress in swiss mice. Pharmacol Res 47:211–216

    Article  CAS  Google Scholar 

  • Beauchamp C, Fridovich I (1971) Superoxide dismutase: improved assays and an assay applicable to acrylamide gel. Anal Biochem 44:276–287

    Article  CAS  Google Scholar 

  • Bougatef A, Nedjar-Arroume N, Manni L, Ravalec-Ple R, Guillochon D, Barkia A, Nasri M (2008c) Identification and characterization of antioxidative peptides from sardinelle (Sardinella aurita) by-products hydrolysates. In: Nasri M (ed) Recent Research Developments in Food by-products Technology and Biotechnology. Research signpost. ISBN: 978–81–308-0259-6, 63–80

  • Bougatef A, Hajji M, Balti R, Lassoued I, Triki-Ellouz Y, Nasri M (2009) Antioxidant and free radical-scavenging activities of smooth hound (Mustelus mustelus) muscle protein hydrolysates obtained by gastrointestinal proteases. Food Chem 114:1198–1205

    Article  CAS  Google Scholar 

  • Bougatef A, Arroume-Nedjar N, Manni L, Ravallec R, Barkia A, Guillochon D, Nasri M (2010) Purification and identification of novel antioxidant peptides from enzymatic hydrolysates of sardinelle (Sardinella aurita) by products proteins. Food Chem 118:559–565

    Article  CAS  Google Scholar 

  • Brocardo PS, Gil-Mohapel J, Christie BR (2011) The role of oxidative stress in fetal alcohol spectrum disorders. Brain Res Rev 67(1–2):209–225

    Article  CAS  Google Scholar 

  • Caro AA, Cederbaum AI (2004) Oxidative stress, toxicology, and pharmacology of CYP2E1. Annu Rev Pharmacol Toxicol 44:27–42

    Article  CAS  Google Scholar 

  • CTA (2004) Centre de Traitement en Alcoologie rattaché au Département Universitaire de Médecine et Santé, Lausanne, Suisse. Communautaires et associé à la Division d’Abus de Substances 1–4

  • Das SK, Vasudevan DM (2006) Protective effects of silymarin, a milk thistle (Silybium marianum) derivative on ethanol-induced oxidative stress in liver. Indian J Biochem Biophys 43:306–311

    CAS  Google Scholar 

  • Draper HH, Hadley M (1990) Malondialdehyde determination as index of lipid peroxidation. Method Enzymol 86:421–431

    Article  Google Scholar 

  • Dreon DM, Krauss RM (1997) Diet-gene interactions in human lipoprotein metabolism. J Am Coll Nut 16(4):313–324

  • Ellman GL (1959) Sulphydryl groups. Arch Biochem Biophys 82:70–77

    Article  CAS  Google Scholar 

  • El-Nekeety AA, El-Kady AA, Soliman MS, Hassan NS, Abdel-Wahhab MA (2009) Protective effect of Aquilegia vulgaris (L.) against lead acetate-induced oxidative stress in rats. Food Chem Toxicol 47(9):2209–2215

    Article  CAS  Google Scholar 

  • Faremi TY, Suru SM, Fafunso MA, Obioha UE (2008) Hepatoprotective potentials of Phyllanus amarus against ethanol-inductive stress in rats. Food Chem Toxicol 46:2658–2664

    Article  CAS  Google Scholar 

  • Flohé L, Gunzler WA (1984) Assays of glutathione peroxidase. Method Enzymol 105:114–121

    Article  Google Scholar 

  • Harper HA (1961) The functions and tests of the liver, review of physiological chemistry. Lange Medical Publishers, Los Atlos, C. A, pp. 271–283

    Google Scholar 

  • Husain K, Scott BR, Reddy SK, Somani SM (2001) Chronic ethanol and nicotine interaction on rat tissue antioxidant defense system. Alcohol 25(2):89–97

    Article  CAS  Google Scholar 

  • Jollow DJ, Mitchell JR, Zampaglione N, Gillete JR (1974) Bromobenzene induced liver necrosis: protective role of glutathione and evidence for 3,4-bromobenzeneoxide as the hepatotoxic intermediate. Pharmacol 11:151–169

    Article  CAS  Google Scholar 

  • Kamoun Z, Kamoun Sellami A, Bougatef A, Chtourou Y, Boudawara T, Nasri M, Zeghal N (2012) Efficacy of sardinelle protein hydrolysate to alleviate ethanol-induced oxidative stress in the heart of adult rats. J Food Sci 77(8):T156–T162

    Article  CAS  Google Scholar 

  • Kasdallah-Grissaa A, Mornagui B, Aouani E, Hammami M, El May M, El Gharbi N, Kamoun A, El Fazaa S (2007) Resveratrol, a red wine polyphenol, attenuates ethanol-induced oxidative stress in rat liver. Life Sci 80(11):1033–1039

    Article  Google Scholar 

  • Kasdallah-Grissaa A, Nakbia A, Koubaa N, El-Fazaâb S, Gharbib N, Kamoun A, Hammamia M (2008) Dietary virgin olive oil protects against lipid peroxidation and improves antioxidant status in the liver of rats chronically exposed to ethanol. Nutr Res 28:472–479

    Article  Google Scholar 

  • Kavitha G, Damodara Reddy V, Paramahamsa M, Akthar PM, Varadacharyulu NC (2008) Role of nitric in alcohol-induced changes in lipid profile of moderate and heavy alcoholics. Alcohol 42:47–53

    Article  CAS  Google Scholar 

  • Kim SK, Kim YT, Byun HG, Nam KS, Joo DS, Shahidi F (2001) Isolation and characterization of antioxidative peptides from gelatine hydrolysate of Alaska Pollak skin. J Agr Food Chem 49:1984–1989

    Article  CAS  Google Scholar 

  • Koch OR, Pani G, Borrello S, Colavitti R, Cravero A, Farre S, Galeotti T (2004) Oxidative stress and antioxidant defenses in ethanol-induced cell injury. Mol Asp Med 25:191–198

    Article  CAS  Google Scholar 

  • Kukielka E, Dicker E, Cederbaum AI (1994) Increased production of reactive oxygen species by rat liver mitochondria after chronic ethanol treatment. Arch Biochem Biophys 309:377–386

    Article  CAS  Google Scholar 

  • Lina X, Zhanga S, Huang Q, Wei L, Zhenga L, Chena Z, Jiaoa Y, Huanga J, Fua S, Huang R (2012) Protective effect of Fufang-Liu-Yue-Qing, a traditional Chinese herbal formula, on CCl4 induced liver fibrosis in rats. J Ethnopharmacol 142:548–556

    Article  Google Scholar 

  • Liu S, Hou W, Ping Y, Zhang B, Sun S, Nüssler AK, Liegag L (2010) Quercetin protects against ethanol-induced oxidative damage in ratprimary hepatocytes. Toxicol in Vitro 24(2):516–522

    Article  CAS  Google Scholar 

  • Lowry OH, Roenbrough NJ, Farr AL, Randall RJ (1951) Protein measurement with the folin phenol reagent. J Biol Chem 193:265–275

    CAS  Google Scholar 

  • Mallikarjuna K, Sahitya-Chetan P, Sathyavelu-Reddy K, Rajendra W (2008) Ethanol toxicity: rehabilitation of hepatic antioxidant defense system with dietary ginger. Fitoterapia 79(3):174–178

    Article  CAS  Google Scholar 

  • Mallikarjuna K, Nishanth K, Hou CW, Kuo CH, Sathyavelu Reddy K (2009) Effect of exercise training on ethanol induced oxidative damage in aged rats. Alcohol 43:59–64

    Article  CAS  Google Scholar 

  • Niemela O (2001) Distribution of ethanol-induced protein adducts in vivo: relationship to tissue injury. Free Radic Biol Med 31:1533–1538

    Article  CAS  Google Scholar 

  • Ojeda ML, Nogales F, Vázquez B, Delgado MJ, Murillo ML, Carreras O (2009) Alcohol, gestation and breastfeeding: selenium as an antioxidant therapy. Alcohol Alcohol 44(3):272–277

    Article  CAS  Google Scholar 

  • Pari L, Suresh A (2008) Effect of grape (Vitis vinifera L.) leaf extract on alcohol induced oxidative stress in rats. Food Chem Toxicol 46:1627–1634

    Article  CAS  Google Scholar 

  • Perez MJ, Velasco E, Monte MJ, Gonzalez-Buitrago JM, Marin JJ (2006) Maternal ethanol consumption during pregnancy enhances bile acid-induced oxidative stress and apoptosis in fetal rat liver. Toxicology 225(2–3):183–194

    Article  CAS  Google Scholar 

  • Preedy VR, Reilly ME, Patel VB, Richardson PJ, Peters TJ (1999) Protein metabolism in alcoholism: effects on specific tissues and the whole body. Nutrition 15:604–608

    Article  CAS  Google Scholar 

  • Rakonczay Z Jr, Boros I, Jarmay K, Hegyi P, Lonovics J, Takacs T (2003) Ethanol administration generates oxidative stress in the pancreas and liver, but fails to induce heat-shock proteins in rats. J Gastroen Hepatol 18(7):858–867

    Article  CAS  Google Scholar 

  • Ronisa JJM, Korourianc S, Yoond S, Sundbergd MI, Albano E, Lindrosf KO, Badgera TM (2004) Lack of sexual dimorphism in alcohol-induced liver damage (ALD) in rats treated chronically with ethanol-containing low carbohydrate diets: the role of ethanol metabolism and endotoxin. Life Sci 75:469–483

    Article  Google Scholar 

  • Saravanan R, Viswanathan P, Pugalendi KV (2006) Protective effect of ursolic acid on ethanol-mediated experimental liver damage in rats. Life Sci 78(7):713–718

    Article  CAS  Google Scholar 

  • Sarkar SN, Chattopadhyay SK, Majumdar AC (1995) Subacute toxicity of urea herbicide, isoproturon in male rats. Indian J Exp Biol 33(11):851–856

    CAS  Google Scholar 

  • Scott R, Reddy KS, Husain K, Schlorff EC, Rybak LP, Somani SM (2007) Dose response of ethanol on antioxidant defense system of liver, lung, and kidney in rat. Pathophysiology 7(1):25–32

    Article  Google Scholar 

  • Sinaceur J, Ribiere C, Sabourault D, Nordman R (1985) Superoxide formation in liver mithochondria during ethanol intoxication: possible role in alcohol hepatotoxicity. In: Poli G, Cheesman KH, Dianzani MU, Slater TF (eds) Free radical in liver injury. IRL Press, Oxford, pp. 175–177

    Google Scholar 

  • Suresh MV, Sreeranjit Kumar CV, Lal JJ, Indira M (1999) Impact of massive ascorbic acid supplementation on alcohol induced oxidative stress in Guinea pigs. Toxicol Lett 104(3):221–229

    Article  CAS  Google Scholar 

  • Tsuge N, Eikawa Y, Nomura Y, Yamamoto M, Sugisawa K (1991) Antioxidant activity of peptides prepared by enzymatic hydrolysis of egg-white albumin Nippon Nogeikagaku Kaishi. J Jpn Soc Biosc Biotechnol Agrochem 65:1635–1641

    CAS  Google Scholar 

  • Tuma DJ, Casey CA (2003) Dangerous by-products of alcohol breakdown-focus on adducts. Alcohol Res Health 27(4):285–290

    Google Scholar 

  • Venkatachalam U, Muthukrishnan S (2013) Hepatoprotective activity of Desmodium gangeticum in paracetamol induced liver damage in rats. Biomed Prev Nutr 3(3):273–277

    Article  Google Scholar 

  • Xu Y, Leo MA, Lieber CS (2003) Lycopene attenuates alcoholic apoptosis in HepG2 cells expressing CYP2E1. Biochem Biophys Res Commun 308:614–618

    Article  CAS  Google Scholar 

  • Yao XH, Nguyen HK, Nyomba BL (2013) Prenatal ethanol exposure causes glucose intolerance with increased hepatic gluconeogenesis and histone deacetylases in adult rat offspring: reversal by tauroursodeoxycholic acid. PLoS One 8(3):1–10

    Google Scholar 

  • You Y, Yoo S, Yoon HG, Park J, Lee YH, Kim S, Oh KT, Lee J, Cho HY, Jun W (2010) In vitro and in vivo hepatoprotective effects of the aqueous extract from Taraxacum officinale (dandelion) root against alcohol-induced oxidative stress. Food Chem Toxicol 48:1632–1637

    Article  CAS  Google Scholar 

  • Yousef MI, El-Demerdash FM, Kamel KI, Al-Salhen KS (2003) Changes in some hematological and biochemical indices of rabbits induced by isoflavones and cypermethrin. Toxicology 189:223–234

    Article  CAS  Google Scholar 

  • Zaahkouk SAM, Helal EGE, Abd-Rabo TEI, Rashed SZA (2000) Carbamate toxicity and protective effect of vit. A and vit. E on some biochemical aspects of male albino rats. Egypt J Hosp Med 1:60–77

    Google Scholar 

  • Zhang H, Wang JZ, Li R, Bai J, Ye YB, Ren FZ (2008) Attenuated effects of peptides derived from porcine plasma albumin on in vitro lipid peroxidation in the liver homogenate of mice. Food Chem 111(2):364–369

    Article  CAS  Google Scholar 

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Acknowledgments

This work was funded by “Ministry of Higher Education, Scientific Research and Technology – Tunisia”.The authors are thankful to Miss Intidhar Bkhairia for her collaboration in this work.

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    Authors and Affiliations

    1. Unité Toxicologie et Microbiologie Environnemental et Santé, Faculté des Sciences de Sfax, BP 1171, 3000, Sfax, Tunisia

      Zeineb Kamoun & Najiba Zeghal

    2. Departement des Sciences de la vie, Université de Sfax-Faculté des Sciences de Sfax, BP 1171, 3000, Sfax, Tunisia

      Zeineb Kamoun

    3. Laboratoire de Génie Enzymatique et de Microbiologie, Ecole Nationale d’Ingénieurs de Sfax, 3038, Sfax, Tunisia

      Alya Sellami Kamoun & Moncef Nasri

    4. Unité Enzymes et Bioconversion, Ecole nationale d’Ingénieurs, BP 1171, 3038, Sfax, Tunisia

      Ali Bougatef

    5. Laboratoire de Biochimie, CHU Hédi Chaker, 3029, Sfax, Tunisia

      Rim Marrakchi Kharrat & Houssem Youssfi

    6. Laboratoire d’Anatomie et de Cytologie Pathologique, CHU Habib Bourguiba, 3023, Sfax, Tunisia

      Tahia Boudawara

    7. Laboratoire Biotechnologie des Plantes Appliquée à l’Amélioration des Cultures, Faculté des Sciences de Sfax, BP 1171, 3000, Sfax, Tunisia

      Mouna Chakroun

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    1. Zeineb Kamoun
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    Correspondence to Zeineb Kamoun.

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    Responsible editor: Philippe Garrigues

    Zeineb Kamoun and Alya Sellami Kamoun contributed equally to the work.

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    Kamoun, Z., Kamoun, A.S., Bougatef, A. et al. Hepatoprotective and nephroprotective effects of sardinelle (Sardinella aurita) protein hydrolysate against ethanol-induced oxidative stress in rats. Environ Sci Pollut Res 24, 1432–1441 (2017). https://doi.org/10.1007/s11356-016-7424-4

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