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Environmental Science and Pollution Research

, Volume 23, Issue 21, pp 21369–21376 | Cite as

Prevalence of bacterial resistance within an eco-agricultural system in Hangzhou, China

  • Like Xu
  • Yanyun Qian
  • Chao Su
  • Weixiao Cheng
  • Jianan Li
  • Mark L. Wahlqvist
  • Hong ChenEmail author
Research Article

Abstract

The wide use of antibiotics in the animal husbandry and the relevant sustainable industries may promote the emergence of antibiotic-resistant bacteria (ARB), which constitutes a growing threat to human health. The objective of this study was to determine the abundance and diversity of sulfonamide- and tetracycline-resistant bacteria within an eco-agricultural system (EAS) in Hangzhou, China. We investigated samples at every link in the EAS, from livestock manure, to biogas residues and biogas slurry, to vegetable and ryegrass fields, to a fish pond. A combination of culture-based and 16S rRNA gene-based sequencing method was used in this study. Within the studied system, the average rate of bacterial resistance to sulfonamide (46.19 %) was much higher than that of tetracycline (8.51 %) (p < 0.01). There were 224 isolates that were enumerated and sequenced, 108 of which were identified to species level. The genera comprising the sulfamethoxazole-resistant (SMXr) bacteria were generally different from those of tetracycline-resistant (TCr) bacteria. Staphylococcus and Acinetobacter were the most dominant genera of SMXr bacteria (19.30 % of the total resistant bacteria) and TCr bacteria (14.04 % of the total resistant bacteria), respectively. Several strains of resistant opportunistic pathogens (e.g., Pantoea agglomerans) were detected in edible vegetable samples, which may exert a potential threat to both pig production and human health. In general, this study indicates that the EAS is an important reservoir of antibiotic-resistant bacteria, some of which may be pathogenic.

Keywords

Eco-agricultural system Sulfonamide Tetracycline Antibiotic-resistant bacteria 

Notes

Acknowledgments

This work was supported by the Major Science and Technology Program for Water Pollution Control and Treatment (2014ZX07101-012) and the Natural Science Foundation of China (Nos. 21277117 and 20210008).

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Copyright information

© Springer-Verlag Berlin Heidelberg 2016

Authors and Affiliations

  • Like Xu
    • 1
  • Yanyun Qian
    • 1
  • Chao Su
    • 1
  • Weixiao Cheng
    • 1
  • Jianan Li
    • 1
  • Mark L. Wahlqvist
    • 2
  • Hong Chen
    • 1
    Email author
  1. 1.Department of Environmental Engineering, College of Environmental and Resource SciencesZhejiang UniversityHangzhouChina
  2. 2.Fuli Institute of Food ScienceZhejiang UniversityHangzhouChina

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