Skip to main content

Advertisement

SpringerLink
Log in

Genotoxic effect of a binary mixture of dicamba- and glyphosate-based commercial herbicide formulations on Rhinella arenarum (Hensel, 1867) (Anura, Bufonidae) late-stage larvae

  • Research Article
  • Published:
Environmental Science and Pollution Research Aims and scope Submit manuscript

Abstract

The acute toxicity of two herbicide formulations, namely, the 57.71 % dicamba (DIC)-based Banvel® and the 48 % glyphosate (GLY)-based Credit®, alone as well as the binary mixture of these herbicides was evaluated on late-stage Rhinella arenarum larvae (stage 36) exposed under laboratory conditions. Mortality was used as an endpoint for determining acute lethal effects, whereas the single-cell gel electrophoresis (SCGE) assay was employed as genotoxic endpoint to study sublethal effects. Lethality studies revealed LC5096 h values of 358.44 and 78.18 mg L−1 DIC and GLY for Banvel® and Credit®, respectively. SCGE assay revealed, after exposure for 96 h to either 5 and 10 % of the Banvel® LC5096 h concentration or 5 and 10 % of the Credit® LC5096 h concentration, an equal significant increase of the genetic damage index (GDI) regardless of the concentration of the herbicide assayed. The binary mixtures of 5 % Banvel® plus 5 % Credit® LC5096 h concentrations and 10 % Banvel® plus 10 % Credit® LC5096 h concentrations induced equivalent significant increases in the GDI in regard to GDI values from late-stage larvae exposed only to Banvel® or Credit®. This study represents the first experimental evidence of acute lethal and sublethal effects exerted by DIC on the species, as well as the induction of primary DNA breaks by this herbicide in amphibians. Finally, a synergistic effect of the mixture of GLY and DIC on the induction of primary DNA breaks on circulating blood cells of R. arenarum late-stage larvae could be demonstrated.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Subscribe and save

Springer+ Basic
$34.99 /Month
  • Get 10 units per month
  • Download Article/Chapter or eBook
  • 1 Unit = 1 Article or 1 Chapter
  • Cancel anytime
Subscribe now

Buy Now

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1

Similar content being viewed by others

Explore related subjects

Discover the latest articles, news and stories from top researchers in related subjects.

References

  • Agostini MG, Natale GS, Ronco AE (2009) Impact of endosulphan and cypermethrin mixture on amphibians under field use for biotech soya bean production. Int J Environ Health 3:379–389

    Article  CAS  Google Scholar 

  • Aronzon CM, Sandoval MT, Herkovits J, Pérez-Coll CS (2011) Stage-dependent toxicity of 2,4-dichlorophenoxyacetic on the embryonic development of a South American toad, Rhinella arenarum. Environ Toxicol 26:373–381

    Article  CAS  Google Scholar 

  • ASIH (2004) Guidelines for use of live amphibians and reptiles in field and laboratory research. Herpetological Animal Care and Use Committee of the American Society of Ichthyologists and Herpetologists, Washington

    Google Scholar 

  • ASTM (2007) Standard guide for conducting acute toxicity tests with fishes, macroinvertebrates, and amphibians. Biological effects and environmental fate. ASTM E 729–96

  • Baas J, Jager T, Kooijman B (2010) A review of DEB theory in assessing toxic effects of mixtures. Sci Total Environ 408:3740–3745

    Article  CAS  Google Scholar 

  • Beebee TJ (2005) Conservation genetics of amphibians. Heredity 95:423–427

    Article  CAS  Google Scholar 

  • Behrens MR, Mutlu N, Chakraborty S, Dumitru R, Wen ZJ, LaVallee BJ, Herman PL, Clemente TE, Weeks DP (2007) Dicamba resistance: enlarging and preserving biotechnology-based weed management strategies. Science 316:1185–1188

    Article  CAS  Google Scholar 

  • Belden JB, Lydy MJ (2000) Impact of atrazine on organophosphate insecticide toxicity. Environ Toxicol Chem 19:2266–2274

    Article  CAS  Google Scholar 

  • Bielecki K, Sarapuk J, Kleszczyńsk H (2004) Binary mixtures of (N-phosphonomethyl)-glycine with new aminophosphonates. Z Naturforsch C 59:515–518

    Article  CAS  Google Scholar 

  • Blouin DC, Webster EP, Wei Z (2004) Analysis of synergistic and antagonistic effects of herbicides using nonlinear mixed-model methodology. Weed Technol 18:464–472

    Article  CAS  Google Scholar 

  • Bolognesi C, Bonatti S, Degan P, Gallerani E, Peluso M, Rabboni R, Roggieri P, Abbondandolo A (1997) Genotoxic activity of glyphosate and its technical formulation Roundup. J Agric Food Chem 45:1957–1962

    Article  CAS  Google Scholar 

  • Bradford DF, Knapp RA, Sparling DW, Nash MS, Stanley KA, Tallent-Halsell NG, McConnell LL, Simonich SM (2011) Pesticide distributions and population declines of California, USA, alpine frogs, Rana muscosa and Rana sierrae. Environ Toxicol Chem 30:682–691

    Article  CAS  Google Scholar 

  • Brodeur JC, Svartz G, Perez-Coll CS, Marino DJ, Herkovits J (2009) Comparative susceptibility to atrazine of three developmental stages of Rhinella arenarum and influence on metamorphosis: non-monotonous acceleration of the time to climax and delayed tail resorption. Aquat Toxicol 91:161–170

    Article  CAS  Google Scholar 

  • Brodeur J, Vera-Candioti J, Soloneski S, Larramendy ML, Ronco AE (2012) Evidence of reduced feeding and oxidative stress in common tree frogs (Hypsiboas pulchellus) from an agroecosystem experiencing severe drought. J Herpertol 46:72–78

    Article  Google Scholar 

  • Brodeur JC, Poliserpi MB, D’Andrea MF, Sánchez M (2014) Synergy between glyphosate- and cypermethrin-based pesticides during acute exposures in tadpoles of the common South American toad Rhinella arenarum. Chemosphere 112:70–76

    Article  CAS  Google Scholar 

  • Brühl CA, Pieper S, Weber B (2011) Amphibians at risk? Susceptibility of terrestrial amphibian life stages to pesticides. Environ Toxicol Chem 30:2465–2472

    Article  Google Scholar 

  • Burlibasa L, Gavrila L (2011) Amphibians as model organisms for study environmental genotoxicity. Appl Ecol Environ Res 9:1–15

    Article  Google Scholar 

  • Cavas T, Konen S (2007) Detection of cytogenetic and DNA damage in peripheral erythrocytes of goldfish (Carassius auratus) exposed to a glyphosate formulation using the micronucleus test and the comet assay. Mutagenesis 22:263–268

    Article  CAS  Google Scholar 

  • Cedergreen N, Streibig JC (2005) The toxicity of herbicides to non-target aquatic plants and algae: assessment of predictive factors and hazard. Pest Manag Sci 61:1152–1160

    Article  CAS  Google Scholar 

  • Collins A, Koppen G, Valdiglesias V, Dusinska M, Kruszewski M, Møler P, Rojas E, Dhawan A, Benzie I, Coskun E, Moretti M, Speit G, Bonassi S (2014) The comet assay as a tool for human biomonitoring studies: the ComNet Project. Mutat Res 759:27–39

    Article  CAS  Google Scholar 

  • de Lapuente J, Lourenço J, Mendo SA, Borràs M, Martins MG, Costa PM, Pacheco M (2015) The comet assay and its applications in the field of ecotoxicology: a mature tool that continues to expand its perspectives. Front Genet 6:180

    Article  Google Scholar 

  • FAO-WHO (2006) Manual on development and use of FAO and WHO specifications for pesticides. FAO/WHO Joint Meeting on Pesticide Specifications (JMPS)

  • Feng S, Kong Z, Wang X, Zhao L, Peng P (2004) Acute toxicity and genotoxicity of two novel pesticides on amphibian, Rana N. Hallowell. Chemosphere 56:457–463

    Article  CAS  Google Scholar 

  • Ferrari A, Lascano C, Pechen De D’Angelo AM, Venturino A (2011) Effects of azinphos methyl and carbaryl on Rhinella arenarum larvae esterases and antioxidant enzymes. Comp Biochem Physiol C Toxicol Pharmacol 153:34–39

    Article  Google Scholar 

  • Finney DJ (1971) Probit analysis. Cambridge Univ. Press, London, 333 pp

    Google Scholar 

  • Furlong ET, Anderson BD, Werner SL, Soliven PP, Coffey LJ, Burkhardt MR (2011) Methods of analysis by the U.S. Geological Survey National Water Quality Laboratory—determination of liquid chromatography/mass spectrometry. U.S. Geological Survey Water Resources Investigations Report 01-4134, 73 pp

  • Gosner KL (1960) A simplified table for staging anuran embryos and larvae with notes on identification. Herpetologica 16:183–190

    Google Scholar 

  • Green JM, Hazel CB, Forney DR, Pugh LM (2008) New multiple-herbicide crop resistance and formulation technology to augment the utility of glyphosate. Pest Manag Sci 64:332–339

    Article  CAS  Google Scholar 

  • Guyton KZ, Loomis DY, El Ghissassi F, Benbrahim-Tallaa L, Guha N, Scoccianti C, Mattock H, Straif K, International Agency for Research on Cancer Monograph Working Group, IARC, Lyon, France (2015) Carcinogenicity of tetrachlorvinphos, parathion, malathion, diazinon, and glyphosate. Lancet Oncol 16:490–491

    Article  Google Scholar 

  • Guzmán JA, Guardia T (1978) Effects of an organophosphorous insecticide on the cholinesteratic activities of Bufo arenarum (H). Bull Environ Contam Toxicol 20:52–58

    Article  Google Scholar 

  • Jones DK, Hammond JI, Relyea RA (2009) Very highly toxic effects of endosulfan across nine species of tadpoles: lag effects and family-level sensitivity. Environ Toxicol Chem 28:1939–1945

    Article  CAS  Google Scholar 

  • Jones DK, Hammond J, Relyea RA (2010) Roundup® and amphibians: the importance of concentration, application time, and stratification. Environ Toxicol Chem 29:2016–2025

    CAS  Google Scholar 

  • Juárez A, Gusmán JA (1984) Chronic effect of five organochlorine insecticides on larvae of Bufo arenarum. Comunic Biol 2:411–416

    Google Scholar 

  • Kehoe L, Kuemmerle T, Meyer C, Levers C, Václavík T, Kreft H (2015) Global patterns of agricultural land-use intensity and vertebrate diversity. Divers Distrib 21:1308–1318

    Article  Google Scholar 

  • Kwet A, Reichle S, Silvano D, Úbeda C, Baldo D, Di Tada I (2004) Rhinella arenarum. The International Union for Conservation of Nature. List of Threatened Species 2004: e.T54576A11169255

  • Lajmanovich RC, Attademo AM, Peltzer PM, Junges CM, Cabagna MC (2011) Toxicity of four herbicide formulations with glyphosate on Rhinella arenarum (Anura: Bufonidae) tadpoles: B-esterases and glutathione S-transferase inhibitors. Arch Environ Contam Toxicol 60:681–689

    Article  CAS  Google Scholar 

  • Lajmanovich RC, Junges CM, Attademo AM, Peltzer PM, Cabagna-Zenklusen MC, Basso A (2013) Individual and mixture toxicity of commercial formulations containing glyphosate, metsulfuron-methyl, bispyribac-sodium, and picloram on Rhinella arenarum tadpoles. Water Air Soil Pollut 224:1404

    Article  Google Scholar 

  • Lascano CI, Ferrari A, Gauna LE, Cocca C, Cochón AC, Verrengia N, Venturino A (2011) Organophosphorus insecticides affect normal polyamine metabolism in amphibian embryogenesis. Pestic Biochem Physiol 101:240–247

    Article  CAS  Google Scholar 

  • Lazarová M, Slameňová D (2004) Genotoxic effects of a complex mixture adsorbed onto ambient air particles on human cells in vitro; the effects of vitamins E and C. Mutat Res 557:167–175

    Article  Google Scholar 

  • Liang HC, Razaviarani V, Buchanan I (2013) Pesticides and herbicides. Water Environ Res 85:1601–1645

    Article  Google Scholar 

  • Lydy M, Belden J, Wheelock C, Hammock B, Denton D (2004) Challenges in regulating pesticide mixtures. Ecol Soc 9:1–13

    Google Scholar 

  • Mann RM, Hyne RV, Choung CB, Wilson SP (2009) Amphibians and agricultural chemicals: review of the risks in a complex environment. Environ Pollut 157:2903–2927

    Article  CAS  Google Scholar 

  • Maselli V, Polese G, Rippa D, Ligrone R, Rastogi RK, Fulgione D (2010) Frogs, sentinels of DNA damage induced by pollution in Naples and the neighbouring provinces. Ecotoxicol Environ Saf 73:1525–1529

    Article  CAS  Google Scholar 

  • Mazzatorta P, Benfenati E, Neagu D, Gini G (2002) The importance of scaling in data mining for toxicity prediction. J Chem Inf Model 42:1250–1255

    CAS  Google Scholar 

  • McCarty LS, Borgert CJ (2006) Review of the toxicity of chemical mixtures containing at least one organochlorine. Regul Toxicol Pharmacol 45:104–118

    Article  CAS  Google Scholar 

  • McLaughlin D, Kinzelbach W (2015) Food security and sustainable resource management. Water Resour Res 51:4966–4985

    Article  Google Scholar 

  • Meffe R, de Bustamante I (2014) Emerging organic contaminants in surface water and groundwater: a first overview of the situation in Italy. Sci Total Environ 481:280–295

    Article  CAS  Google Scholar 

  • Meza-Joya FL, Ramírez-Pinilla MP, Fuentes-Lorenzo JL (2013) Toxic, cytotoxic, and genotoxic effects of a glyphosate formulation (Roundup®SL-Cosmoflux®411F) in the direct-developing frog Eleutherodactylus johnstonei. Environ Mol Mutagen 54:362–373

    Article  CAS  Google Scholar 

  • Moore LJ, Fuentes L, Rodgers JHJ, Bowerman WW, Yarrow GK, Chao WY, Bridges WCJ (2012) Relative toxicity of the components of the original formulation of Roundup to five North American anurans. Ecotoxicol Environ Saf 78:128–133

    Article  CAS  Google Scholar 

  • Mouchet F, Gauthier L, Baudrimont M, Gonzalez P, Mailhes C, Ferrier V, Devaux A (2007) Comparative evaluation of the toxicity and genotoxicity of cadmium in amphibian larvae (Xenopus laevis and Pleurodeles waltl) using the comet assay and the micronucleus test. Environ Toxicol 22:422–435

    Article  CAS  Google Scholar 

  • Natale GS, Ammassari LL, Basso NG, Ronco AE (2006) Acute and chronic effects of Cr(VI) on Hypsiboas pulchellus embryos and tadpoles. Dis Aquat Org 72:261–267

    Article  CAS  Google Scholar 

  • Nikoloff N, Larramendy ML, Soloneski S (2014a) Comparative evaluation in vitro of the herbicide flurochloridone by cytokinesis-block micronucleus cytome and comet assays. Environ Toxicol 29:884–892

    Article  CAS  Google Scholar 

  • Nikoloff N, Larramendy ML, Soloneski S (2014b) Assessment of DNA damage, cytotoxicity, and apoptosis in human hepatoma (HepG2) cells after flurochloridone herbicide exposure. Food Chem Toxicol 65:233–241

    Article  CAS  Google Scholar 

  • Nikoloff N, Natale GS, Marino D, Soloneski S, Larramendy M (2014c) Flurochloridone-based herbicides induced genotoxicity effects on Rhinella arenarum tadpoles (Anura: Bufonidae). Ecotoxicol Environ Saf 100:275–281

    Article  CAS  Google Scholar 

  • Olszyk D, Pfleeger T, Lee EH, Plocher M (2015) Glyphosate and dicamba herbicide tank mixture effects on native plant and non-genetically engineered soybean seedlings. Ecotoxicology 24:1014–1027

    Article  CAS  Google Scholar 

  • Peixoto F (2005) Comparative effects of the Roundup and glyphosate on mitochondrial oxidative phosphorylation. Chemosphere 61:1115–1122

    Article  CAS  Google Scholar 

  • Pereira JL, Antunes SC, Castro BB, Marques CR, Gonçalves AMM, Gonçalves F, Pereira R (2009) Toxicity evaluation of three pesticides on non-target aquatic and soil organisms: commercial formulation versus active ingredient. Ecotoxicology 18:455–463

    Article  CAS  Google Scholar 

  • Pérez-Iglesias JM, Ruiz de Arcaute C, Nikoloff N, Dury L, Soloneski S, Natale GS, Larramendy M (2014) The genotoxic effects of the imidacloprid-based insecticide formulation Glacoxan Imida on Montevideo tree frog Hypsiboas pulchellus tadpoles (Anura, Hylidae). Ecotoxicol Environ Saf 104:120–126

    Article  Google Scholar 

  • Pérez-Iglesias JM, Soloneski S, Nikoloff N, Natale GS, Larramendy ML (2015) Toxic and genotoxic effects of the imazethapyr-based herbicide formulation Pivot H® on montevideo tree frog Hypsiboas pulchellus tadpoles (Anura, Hylidae). Ecotoxicol Environ Saf 119:15–24

    Article  Google Scholar 

  • Pettersson M, Ekelund NGA (2006) Effects of the herbicides Roundup and Avans on Euglena gracilis. Arch Environ Contam Toxicol 50:175–181

    Article  CAS  Google Scholar 

  • Pimentel D, McLaughlin L, Zepp A, Lakitan B, Kraus T, Kleinman P, Vancini F, Roach WJ, Graap E, Keeton WS, Selig G (1993) Environmental and economic effects of reducing pesticide use in agriculture. Agric Ecosyst Environ 46:273–288

    Article  CAS  Google Scholar 

  • Pitarque M, Vaglenov A, Nosko M, Hirvonen A, Norppa H, Creus A, Marcos R (1999) Evaluation of DNA damage by the comet assay in shoe workers exposed to toluene and other organic solvents. Mutat Res 441:115–127

    Article  CAS  Google Scholar 

  • Ralph S, Petras M (1997) Genotoxicity monitoring of small bodies of water using two species of tadpoles and the alkaline single cell gel (comet) assay. Environ Mol Mutagen 29:418–430

    Article  CAS  Google Scholar 

  • Relyea RA (2009) A cocktail of contaminants: how mixtures of pesticides at low concentrations affect aquatic communities. Oecologia 159:363–376

    Article  Google Scholar 

  • Rodney SI, Teed RS, Moore DRJ (2013) Estimating the toxicity of pesticide mixtures to aquatic organisms: a review. Hum Ecol Risk Assess 19:1557–1575

    Article  CAS  Google Scholar 

  • Roustan A, Aye M, De Meo M, Di Giorgio C (2014) Genotoxicity of mixtures of glyphosate and atrazine and their environmental transformation products before and after photoactivation. Chemosphere 108:93–100

    Article  CAS  Google Scholar 

  • Ruiz de Arcaute C, Pérez-Iglesias JM, Nikoloff N, Natale GS, Soloneski S, Larramendy M (2014) Genotoxicity evaluation of the insecticide imidacloprid on circulating blood cells of Montevideo tree frog Hypsiboas pulchellus tadpoles (Anura, Hylidae) by comet and micronucleus bioassays. Ecol Indic 45:632–639

    Article  CAS  Google Scholar 

  • Santos M, Morgado R, Ferreira NG, Soares AM, Loureiro S (2011) Evaluation of the joint effect of glyphosate and dimethoate using a small-scale terrestrial ecosystem. Ecotoxicol Environ Saf 74:1994–2001

    Article  CAS  Google Scholar 

  • Singh NP (1996) Microgel electrophoresis of DNA from individual cells: principles and methodology. In: Pfeifer GP (ed) Technologies for detection of DNA damage and mutations. Plenum, New York, pp 3–24

    Chapter  Google Scholar 

  • Sobrero MC, Rimoldi F, Ronco AE (2007) Effects of the glyphosate active ingredient and a formulation on Lemna gibba L. at different exposure levels and assessment end-points. Bull Environ Contam Toxicol 79:537–543

    Article  CAS  Google Scholar 

  • Soloneski S, Larramendy M (2010) Sister chromatid exchanges and chromosomal aberrations in Chinese Hamster Ovary (CHO-K1) cells treated with insecticide pirimicarb. J Hazard Mater 174:410–415

    Article  CAS  Google Scholar 

  • Soloneski S, Reigosa MA, Molinari G, González NV, Larramendy ML (2008) Genotoxic and cytotoxic effects of carbofuran and Furadan® on Chinese hamster ovary (CHOK1) cells. Mutat Res 656:68–73

    Article  CAS  Google Scholar 

  • Soloneski S, Kujawski M, Scuto A, Larramendy ML (2015) Carbamates: a study on genotoxic, cytotoxic, and apoptotic effects induced in Chinese hamster ovary (CHO-K1) cells. Toxicol In Vitro 29:834–844

    Article  CAS  Google Scholar 

  • Sotomayor V, Chiriotto TS, Pechen AM, Venturino A (2015) Biochemical biomarkers of sublethal effects in Rhinella arenarum late gastrula exposed to the organophosphate chlorpyrifos. Pestic Biochem Physiol 119:48–53

    Article  CAS  Google Scholar 

  • Tatum VE, Borton D, Streblow R, Louch J, Shepard JP (2012) Acute toxicity of commonly used forestry herbicide mixtures to Ceriodaphnia dubia and Pimephales promelas. Environ Toxicol 27:671–684

    Article  CAS  Google Scholar 

  • Tsui MTK, Chu LM (2003) Aquatic toxicity of glyphosate-based formulations: comparison between different organisms and the effects of environmental factors. Chemosphere 52:1189–1197

    Article  CAS  Google Scholar 

  • Tsui MTK, Chu LM (2008) Environmental fate and non-target impact of glyphosate-based herbicide (Roundup®) in a subtropical wetland. Chemosphere 71:439–446

    Article  CAS  Google Scholar 

  • UN (2011) Peligros para el medio ambiente. Naciones Unidas Parte 4:229–258

    Google Scholar 

  • U.S. EPA (1975) Methods for acute toxicity tests with fish, macroinvertebrates, and amphibians. USEPA 660/3-75-009, 62

  • U.S. EPA (1982) Pesticide Assessment Guidelines Subdivision E. Hazard evaluation: wildlife and aquatic organisms. United States Environmental Protection Agency, Washington DC EPA-540/9-82-024

  • U.S. EPA (1989) Short-term methods for estimating the chronic toxicity of effluents and receiving waters to aquatic organisms. 2nd ed. United States Environmental Protection Agency Report No. EPA/600/4-89/001

  • U.S. EPA (2001) United States Environmental Protection Agency Report. Environmental hazard assessment and ecological risk assessment methodology. Appendix H

  • U.S. EPA (2002) Methods for measuring the acute toxicity of effluents and receiving waters to freshwater and marine organisms. Fifth edition. USEPA 821-R-02-012

  • Vera Candioti J, Natale G, Soloneski S, Ronco AE, Larramendy ML (2010) Sublethal and lethal effects on Rhinella arenarum (Anura, Bufonidae) tadpoles exerted by the pirimicarb-containing technical formulation insecticide Aficida®. Chemosphere 78:249–255

    Article  Google Scholar 

  • Wagner N, Reichenbecher W, Teichmann H, Tappeser B, Lötters S (2013) Questions concerning the potential impact of glyphosate-based herbicides on amphibians. Environ Toxicol Chem 32:1688–1700

    Article  CAS  Google Scholar 

  • Wagner N, Züghart W, Mingo V, Lötters S (2014) Are deformation rates of anuran developmental stages suitable indicators for environmental pollution? Possibilities and limitations. Ecol Indic 45:394–401

    Article  CAS  Google Scholar 

  • Wagner N, Lötters S, Veith M, Viertel B (2015) Effects of an environmentally relevant temporal application scheme of low herbicide concentrations on larvae of two anuran species. Chemosphere 135:175–181

    Article  CAS  Google Scholar 

  • Warne MSJ (2003) A review of the ecotoxicity of mixtures, approaches to, and recommendations for, their management. In: Langley A, Gilbey M, Kennedy B (eds) Proceedings of the fifth national workshop on the assessment of site contamination. National Environment Protection Council Service Corporation, Adelaide, pp 253–276

    Google Scholar 

  • WHO (1990) Public health impacts of pesticides used in agriculture (WHO in collaboration with the United Nations Environment Programme, Geneva, 1990). World Health Organization

  • WHO (2009) The WHO recommended classification of pesticides by hazard. World Health Organ 1:1–81

    Google Scholar 

  • WHO-FAO (1997) Pesticides residues in food. World Health Organization and Food and Agriculture Organization of the United Nations, Rome

  • Yin XH, Li SN, Zhang L, Zhu GN, Zhuang HS (2008) Evaluation of DNA damage in Chinese toad (Bufo bufo gargarizans) after in vivo exposure to sublethal concentrations of four herbicides using the comet assay. Ecotoxicology 17:280–286

    Article  CAS  Google Scholar 

  • Zeljezic D, Garaj-Vrhovac V, Perkovic P (2006) Evaluation of DNA damage induced by atrazine and atrazine-based herbicide in human lymphocytes in vitro using a comet and DNA diffusion assay. Toxicol In Vitro 20:923–935

    Article  CAS  Google Scholar 

Download references

Acknowledgments

This study was supported by grants from the National University of La Plata (Grants 11/N699 and 11/N746) and the National Council for Scientific and Technological Research (CONICET, PIP No. 0344) from Argentina.

Author information

Authors and Affiliations

  1. Cátedra de Citología, Facultad de Ciencias Naturales y Museo, Universidad Nacional de La Plata, Calle 64 Nro. 3 (esq. 120), B1904AMA, La Plata, Argentina

    Sonia Soloneski, Celeste Ruiz de Arcaute & Marcelo L. Larramendy

  2. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Buenos Aires, Argentina

    Sonia Soloneski, Celeste Ruiz de Arcaute & Marcelo L. Larramendy

Authors
  1. Sonia Soloneski
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Celeste Ruiz de Arcaute
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Marcelo L. Larramendy
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Marcelo L. Larramendy.

Ethics declarations

Conflict of interest

The authors declare that there are no conflicts of interest.

Additional information

Responsible editor: Philippe Garrigues

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Soloneski, S., Ruiz de Arcaute, C. & Larramendy, M.L. Genotoxic effect of a binary mixture of dicamba- and glyphosate-based commercial herbicide formulations on Rhinella arenarum (Hensel, 1867) (Anura, Bufonidae) late-stage larvae. Environ Sci Pollut Res 23, 17811–17821 (2016). https://doi.org/10.1007/s11356-016-6992-7

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11356-016-6992-7

Keywords

Search

Navigation