Environmental Science and Pollution Research

, Volume 23, Issue 17, pp 17811–17821

Genotoxic effect of a binary mixture of dicamba- and glyphosate-based commercial herbicide formulations on Rhinella arenarum (Hensel, 1867) (Anura, Bufonidae) late-stage larvae

  • Sonia Soloneski
  • Celeste Ruiz de Arcaute
  • Marcelo L. Larramendy
Research Article

DOI: 10.1007/s11356-016-6992-7

Cite this article as:
Soloneski, S., Ruiz de Arcaute, C. & Larramendy, M.L. Environ Sci Pollut Res (2016) 23: 17811. doi:10.1007/s11356-016-6992-7

Abstract

The acute toxicity of two herbicide formulations, namely, the 57.71 % dicamba (DIC)-based Banvel® and the 48 % glyphosate (GLY)-based Credit®, alone as well as the binary mixture of these herbicides was evaluated on late-stage Rhinella arenarum larvae (stage 36) exposed under laboratory conditions. Mortality was used as an endpoint for determining acute lethal effects, whereas the single-cell gel electrophoresis (SCGE) assay was employed as genotoxic endpoint to study sublethal effects. Lethality studies revealed LC5096 h values of 358.44 and 78.18 mg L−1 DIC and GLY for Banvel® and Credit®, respectively. SCGE assay revealed, after exposure for 96 h to either 5 and 10 % of the Banvel® LC5096 h concentration or 5 and 10 % of the Credit® LC5096 h concentration, an equal significant increase of the genetic damage index (GDI) regardless of the concentration of the herbicide assayed. The binary mixtures of 5 % Banvel® plus 5 % Credit® LC5096 h concentrations and 10 % Banvel® plus 10 % Credit® LC5096 h concentrations induced equivalent significant increases in the GDI in regard to GDI values from late-stage larvae exposed only to Banvel® or Credit®. This study represents the first experimental evidence of acute lethal and sublethal effects exerted by DIC on the species, as well as the induction of primary DNA breaks by this herbicide in amphibians. Finally, a synergistic effect of the mixture of GLY and DIC on the induction of primary DNA breaks on circulating blood cells of R. arenarum late-stage larvae could be demonstrated.

Keywords

Amphibians Dicamba-based formulations Glyphosate-based formulations Lethal effects SCGE assay 

Funding information

Funder NameGrant NumberFunding Note
Universidad Nacional de La Plata
  • 11/N699
  • 11/N746
National Council for Scientific and Technological Research
  • PIP0344

Copyright information

© Springer-Verlag Berlin Heidelberg 2016

Authors and Affiliations

  • Sonia Soloneski
    • 1
    • 2
  • Celeste Ruiz de Arcaute
    • 1
    • 2
  • Marcelo L. Larramendy
    • 1
    • 2
  1. 1.Cátedra de Citología, Facultad de Ciencias Naturales y MuseoUniversidad Nacional de La PlataLa PlataArgentina
  2. 2.Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET)Buenos AiresArgentina

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