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Evaluation of the joint toxicity of chlorpyrifos ethyl and fenobucarb on climbing perch (Anabas testudineus) from rice fields in the Mekong Delta, Vietnam

Abstract

Organophosphates (e.g. chlorpyrifos ethyl) and carbamates (e.g. fenobucarb) are commonly used to control a wide range of pests in rice fields of the Mekong Delta in Vietnam. This study assesses the combined effect of chlorpyrifos ethyl (CPF) and fenobucarb (F), applied at concentrations used by rice farmers, on the brain acetylcholinesterase (AChE) activity in climbing perch fingerlings from rice fields in the Mekong Delta. It also investigates if Pyridine-2-aldoxime methiodide (2-PAM) can be used to reactivate cholinesterase that has been blocked by CPF. Three days after spraying, the water concentrations of both insecticides decreased quickly below the detection levels. However, the brain AChE activity in fish was inhibited for more than 7 days. The results indicate a quicker but less prolonged inhibition of the brain AChE activity by the mixture than by only CPF. The inhibition levels were above 70 % only during the first 48 h, which could explain why all fish survived. 2-PAM resulted in a significant reactivation of the cholinesterase blocked by a combination of CPF and F, and it is proposed that the reactivation by 2-PAM could provide a way to assess the AChE inhibition levels in organisms, when no unexposed individuals are available as controls. The results indicate that the current use of CPF and F in rice farming in the Mekong Delta is likely to cause negative effects on non-target organisms. Many of these effects may be sub-lethal, and there is a need to develop biomarkers that are relevant, inexpensive and easy to apply. The results show that brain AChE in climbing perch fingerling is a relevant biomarker for monitoring of exposure to, and sub-lethal impacts from organophosphates and carbamates under tropical conditions in developing countries.

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References

  • Anh VT, Chiem NN, Dung DT, Sultana P (2003) Vietnam PRA Report. Understanding livelihoods dependent on inland fisheries in Bangladesh and South East Asia—Synthesis Report for FMSP Project R8118. World Fish Center

  • Assis CRD, Bezerra RS, Luiz B, Carvalho, Jr (2011) Fish Cholinesterases as Biomarkers of Organophosphorus and Carbamate Pesticides. In: Stoytcheva M (ed) Pesticides in the Modern World - Pests Control and Pesticides Exposure and Toxicity Assessment. InTech, p 626. doi:10.5772/20777

  • Baensch HA, Riehl R (1982) Aquarium Atlas, vol 1. Mergus Verlag, Melle, Germany

    Google Scholar 

  • Belden JB, Lydy MJ (2000) Impact of atrazine on organophosphate insecticide toxicity. Environ Toxicol Chem 19:2266–2274. doi:10.1002/etc.5620190917

    Article  CAS  Google Scholar 

  • Berg H, Berg C, Nguyen TT (2012) Integrated rice-fish farming: safeguarding biodiversity and ecosystem services for sustainable food production in the Mekong Delta. J Sustain Agric 36:859–872. doi:10.1080/10440046.2012.712090

    Article  Google Scholar 

  • Bondarenko S, Gan J (2004) Degradation and sorption of selected organophosphate and carbamate insecticides in urban stream sediments. Environ Toxicol Chem 23:1809–1814

    Article  CAS  Google Scholar 

  • Boonthai C, Scott RR, Chapman RB (2000) Acetylcholinesterase as a biomarker to assess the effect of chlorpyrifos and atrazine on some New Zealand aquatic invertebrates. Australas J Ecotoxicol 6:59–64

    CAS  Google Scholar 

  • Chen C, Wang Y, Zhao X, Wang Q, Qian Y (2014) The combined toxicity assessment of carp (Cyprinus carpio) acetylcholinesterase activity by binary mixtures of chlorpyrifos and four other insecticides. Ecotoxicology 23:221–228. doi:10.1007/s10646-013-1165-7

    Article  CAS  Google Scholar 

  • Čolović MB, Krstić DZ, Lazarević-Pašti TD, Bondžić AM, Vasić VM (2013) Acetylcholinesterase inhibitors: pharmacology and toxicology. Curr Neuropharmacol 11:315–335. doi:10.2174/1570159X11311030006

    Article  Google Scholar 

  • Cong NV, Phuong NT, Bayley M (2006) Sensitivity of brain cholinesterase activity to diazinon (BASUDIN 50EC) and fenobucarb (BASSA 50EC) insecticides in the air-breathing fish Channa striata (Bloch, 1793). Environ Toxicol Chem 25:1418–1425

    Article  Google Scholar 

  • Cong NV, Phuong NT, Bayley M (2008) Brain cholinesterase response in the snakehead fish (Channa striata) after field exposure to diazinon. Ecotoxicol Environ Saf 71:314–318. doi:10.1016/j.ecoenv.2008.04.005

    Article  CAS  Google Scholar 

  • Dasgupta S, Meisner C, Wheeler D, Xuyen K, Lam NT (2007) Pesticide poisoning of farm workers—implications of blood test results from Vietnam. Int J Hyg Environ Health 210:121–132. doi:10.1016/j.ijheh.2006.08.006

    Article  CAS  Google Scholar 

  • Darvesh S, Darvesh KV, McDonald RS, Mataija D, Walsh R, Lockridge S, Mothana O, Martin E (2008) Carbamates with differential mechanism of inhibition toward acetylcholinesterase and butyrylcholinesterase. J Med Chem 51:4200–4212

    Article  CAS  Google Scholar 

  • Edwards P, Little DC, Yakupitiyage A (1997) A comparison of traditional and modified inland artisanal aquaculture systems. Aquacul Res 28:777–788. doi:10.1046/j.1365-2109.1997.00942.x

    Article  Google Scholar 

  • Ellman GL, Courtney KD, Andres VJ, Feather-Stone RM (1961) A new and rapid colorimetric determination of acetylcholinesterase activity. Biochem Pharmacol 7:88–95. doi:10.1016/0006-2952(61)90145-9

    Article  CAS  Google Scholar 

  • Ferrari A, Venturino A, de D’Angelo AM (2004) Time course of brain cholinesterase inhibition and recovery following acute and subacute azinphosmethyl, parathion and carbaryl exposure in the goldfish (Carassius auratus). Ecotoxicol Environ Saf 57:420–425. doi:10.1016/S0147-6513(02)00069-6

    Article  CAS  Google Scholar 

  • Fulton MH, Key PB (2001) Acetylcholinesterase inhibition in estuarine fish and invertebrates as an indicator of organophosphorus insecticide exposure and effects. Environ Toxicol Chem 20:37–45. doi:10.1897/1551-5028(2001)020<0037:Aiiefa>2.0.Co;2

    Article  CAS  Google Scholar 

  • Fukuto TR (1990) Mechanism of action of organophosphorus and carbamate insecticides. Environ Health Perspect 87:245–254

    Article  CAS  Google Scholar 

  • Gruber SJ, Munn MD (1998) Organophosphate and carbamate insecticides in agricultural waters and cholinesterase (ChE) inhibition in common carp (Cyprinus carpio). Arch Environ Contam Toxicol 35:391–396. doi:10.1007/s002449900393

    Article  CAS  Google Scholar 

  • Hai TV (2005) Syllabus of chemical plant protection. Faculty of Agriculture, University of Can Tho, Vietnam

    Google Scholar 

  • Heong KL, Escalada MM, Mai V (1994) An analysis of insecticide use in rice: case studies in the Philippines and Vietnam. Int J Pest Manag 40:173–178. doi:10.1080/09670879409371878

    Article  Google Scholar 

  • Herre AW (1952) Philippine fish tales. D.P. Perez Company, Manila, Philippines

    Google Scholar 

  • Klemick H, Lichtenberg E (2008) Pesticide use and fish harvests in Vietnamese rice agroecosystems. Am J Agr Econ 90:1–14. doi:10.1111/j.1467-8276.2007.01059.x

    Article  Google Scholar 

  • Labenia JS, Baldwin DH, French BL, Davis JW, Scholz NL (2007) Behavioral impairment and increased predation mortality in cutthroat trout exposed to carbaryl. Mar Ecol: Prog Ser 329:1–11

    Article  CAS  Google Scholar 

  • Laetz CA, Baldwin DH, Collier TK, Hebert V, Stark JD, Scholz NL (2009) The synergistic toxicity of pesticide mixtures: implications for risk assessment and the conservation of endangered pacific salmon. Environ Health Perspect 117:348–353. doi:10.1289/ehp.0800096

    Article  CAS  Google Scholar 

  • Laetz CA, Baldwin DH, Hebert V, Stark JD, Scholz NL (2013) Interactive Neurobehavioral toxicity of diazinon, malathion, and ethoprop to juvenile coho salmon. Environ Sci Technol 47:2925–2931. doi:10.1021/es305058y

    Article  CAS  Google Scholar 

  • Le Coq J-F, Trebuil G (2005) Impact of economic liberalization on rice intensification, agricultural diversification, and rural livelihoods in the Mekong Delta, Vietnam. Southeast Asian Studies

  • MARD (2013) List of pesticides permitted, restricted, and banned for use. Decision No. 21/2013/TT-BNNPTNT issued on April 17, 2013. Ministry of Agriculture and Rural Development, Ha Noi

    Google Scholar 

  • MARD (2014) Ministry of Agriculture and Rural Development. Statistics and food security data bases. Cultivation data. http://fsiu.mard.gov.vn/data/trongtrot.htm. Assessed 14 Feb 2015

  • Mehler TW, Schuler LJ, Lydy MJ (2007) Examining the joint toxicity of chlorpyrifos and atrazine in the aquatic species: Lepomis macrochirus, Pimephales promelas and Chironomus tentans. Environ Pollut 152:217–224

    Article  CAS  Google Scholar 

  • Nam CQ (2011) Evaluation of rotational rice-fish farming systems and new fish species for concurrent rice-fish culture in the Mekong Delta, Vietnam. Dissertation, K.U.Leuven

  • Nam CQ, Vromant N, Be TT, Ollevier F (2012) Investigation of the predation potential of different fish species on brown planthopper (Nilaparvata lugens (Stål)) in experimental rice-fish aquariums and tanks. Crop Prot 38:95–102. doi:10.1016/j.cropro.2012.03.003

    Article  Google Scholar 

  • Nunes B (2011) The use of cholinesterases in ecotoxicology. Rev Environ Contam Toxicol 212:29–59

    CAS  Google Scholar 

  • Parfitt CH (2000) Pesticide and industrial chemical residues. In: Horwitz W (ed) Official Methods of Analysis of AOAC International, vol 1—Agrochemicals; Contaminants; Drugs, 17th edn. Association of Analytical Communities International, Gaithersburg, pp 22–23

    Google Scholar 

  • Rainboth WJ (1996) Fishes of the Cambodian Mekong. Food and Agriculture Organization of the United Nations, Rome, Italia

    Google Scholar 

  • Sakurada K, Ikegaya H, Ohta H, Akutsu T, Takatori T (2006) Hydrolysis of an acetylthiocholine by pralidoxime iodide (2-PAM). Toxicol Lett 166:255–260. doi:10.1016/j.toxlet.2006.07.339

    Article  CAS  Google Scholar 

  • Sandahl JF, Baldwin DH, Jenkins JJ, Scholz NL (2005) Comparative thresholds for acetylcholinesterase inhibition and behavioral impairment in coho salmon exposed to chlorpyrifos. Environ Toxicol Chem 24:136--145

  • Sebesvari Z, Le H, Toan PV, Arnold U, Renaud F (2012) Agriculture and water quality in the Vietnamese Mekong Delta. In: Renaud FG, Kuenzer C (eds) The Mekong Delta System. Springer Environmental Science and Engineering. Springer, Netherlands, pp 331–361. doi:10.1007/978-94-007-3962-8_13

    Chapter  Google Scholar 

  • Silman I, Sussman JL (2005) Acetylcholinesterase: ‘classical’ and ‘non-classical’ functions and pharmacology. Curr Opin Pharmacol 5:293–302

    Article  CAS  Google Scholar 

  • Singh S, Chaudhry D, Behera D, Gupta D, Jindal SK (2001) Aggressive atropinisation and continuous pralidoxime (2-PAM) infusion in patients with severe organophosphate poisoning: experience of a northwest Indian hospital. Hum Exp Toxicol 20:15–18

    Article  CAS  Google Scholar 

  • Stadlinger N, Berg H, Tam NT, Gunnarsson JS, Van den Brink P (2016) Predicted toxicity risks of pesticides used in paddy rice field in the Mekong and comparison between different agro- Delta, Vietnam, management regimes. Accepted Environ Sci Pollut R

  • Tam NT, Berg H, Hang NTT, Cong NV (2015) Effects of Chlorpyrifos ethyl on acetylcholinesterase activity in Climbing perch cultured in rice fields in the Mekong Delta, Vietnam. Ecotoxicol Environ Saf 117:34–40. doi:10.1016/j.ecoenv.2015.03.020

    Article  CAS  Google Scholar 

  • Tam NT (2016) Pesticides use in rice farming and its impacts on the brain AChE activity in climbing perch (Anabas testudineus) in the Mekong Delta of Vietnam. Dissertation, Stockholm University

  • Tam NT, Berg H, Lauraeus J, Cong NV, Tedengren M (2016) Effects of sequential applications of Bassa 5EC (Fenobucarb) and Vitashiled 40EC (Chlorpyrifos ethyl) on acetylcholinesterase activity in Climbing perch (Anabas testudineus) cultured in rice fields in the Mekong Delta, Vietnam. Arch Environ Contam Toxicol. doi: 10.1007/s00128-016-1796-5

  • Taylor B, Brown JH (1999) Acetylcholine. In: Siegel GJ, Agranoff BW, Albers RW, Fisher SK, Uhler MD (eds) Basic neurochemistry: molecular, cellular, and medical aspects. Lippincott Williams & Wilkins, Philadelphia, PA, USA, pp 213–242

    Google Scholar 

  • Toan PV, Sebesvari Z, Blasing M, Rosendahl I, Renaud FG (2013) Pesticide management and their residues in sediments and surface and drinking water in the Mekong Delta, Vietnam. Sci Total Environ 452:28–39. doi:10.1016/j.scitotenv.2013.02.026

    Article  CAS  Google Scholar 

  • Vromant N, Chau NH, Ollevier F (2001) The effect of rice-seeding rate and fish stocking on the floodwater ecology of the trench of a concurrent, direct-seeded rice-fish system. Hydrobiologia 457:105–117. doi:10.1023/a:1012218725126

    Article  Google Scholar 

  • WHO (2005) WHO Specifications and Valuations for Public Health Pesticides Propoxur. 2-isopropoxyphenyl methylcarbamate. World Health Organization, Geneva

    Google Scholar 

  • Xuan VT, Matsui S (1998) Development of Farming Systems in the Mekong Delta of Vietnam. JIRCAS, CTU & CLRRI. Ho Chi Minh Publishing House, Ho Chi Minh, Vietnam

    Google Scholar 

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Acknowledgments

This study was made with financial support from Swedish International Development Cooperation Agency (Sida) (Ref. No. AKT2010-010) through the Partner Driven Cooperation (PDC) project “Managing Ecosystem Services for Sustainable Aquaculture Development. The study also funded by the Ministry of Education and Training of Vietnam (Ref. No 4069/QĐ-BGDĐT). The manuscript was improved with valuable comments from three reviewers.

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Correspondence to Nguyen Thanh Tam.

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Tam, N.T., Berg, H. & Van Cong, N. Evaluation of the joint toxicity of chlorpyrifos ethyl and fenobucarb on climbing perch (Anabas testudineus) from rice fields in the Mekong Delta, Vietnam. Environ Sci Pollut Res 25, 13226–13234 (2018). https://doi.org/10.1007/s11356-016-6980-y

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  • DOI: https://doi.org/10.1007/s11356-016-6980-y

Keywords

  • Anabas testudineus
  • Chlorpyrifos ethyl
  • Fenobucarb
  • Mixture
  • Acetylcholinesterase
  • Mekong Delta