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Structural analysis, and antioxidant and antibacterial properties of chitosan-poly (vinyl alcohol) biodegradable films

Environmental Science and Pollution Research volume 23, pages 15310–15320 (2016)Cite this article

Abstract

The development and characterization of biodegradable blend films based on chitosan and poly (vinyl alcohol) for possible use in a variety of biological activities are reported. Fourier transform infrared spectroscopy (FTIR) spectra of chitosan-poly (vinyl alcohol) (Ch/PVA) films showed characteristics peaks shifting to a lower frequency range due to hydrogen bonding between −OH of PVA and −NH2 of chitosan. The chitosan and PVA polymers presented good compatibility. The morphology study of chitosan and composite films showed a compact and homogenous structure. The tensile strength and elongation at break increased with PVA content. In fact, the highest tensile strength and elongation at break (53.58 MPa and 454 %) occurs with pure PVA film. The results showed that PVA incorporation in the blends contributes to increase the intermolecular interactions, thus improving the mechanical properties. In addition, the prepared films demonstrated high antioxidant activities monitored by 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical-scavenging, reducing power, and β-carotene bleaching activity. Nevertheless, PVA addition reduced antioxidant and antibacterial activities against Gram-positive and Gram-negative bacteria tested.

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References

  • Abdelrazek EM, Elashmawi IS, Labeeb S (2010) Chitosan filler effects on the experimental characterization, spectroscopic investigation and thermal studies of PVA/PVP blend films. Phys B Condens Matter 405:2021–2027

    CAS  Article  Google Scholar 

  • Bahrami SB, Kordestani SS, Mirzadeh H, Mansoori P (2003) Poly (vinyl alcohol)-chitosan blends: preparation, mechanical and physical properties. Iran Polym J 12:139–146

    CAS  Google Scholar 

  • Bersuder P, Hole M, Smith G (1998) Antioxidant from a heated histidine- glucose model system. I: investigation of the antioxidant role of histidine and isolation of antioxidants by high performance liquid chromatography. J Am Oil Chem Soc 75:181–187

    CAS  Article  Google Scholar 

  • Bonilla J, Fortunati E, Atarés L, Chiralt A, Kenny JM (2014) Physical, structural and antimicrobial properties of polyvinyl alcohol-chitosan biodegradable films. Food Hydrocoll 35:463–470

    CAS  Article  Google Scholar 

  • Buraidah MH, Arof AK (2011) Characterization of chitosan/PVA blended electrolyte doped with NH4I. J Non-Cryst Solids 357:3261–3266

    CAS  Article  Google Scholar 

  • Casariego A, Souza BWS, Vicente AA, Teixeira JA, Cruz L, Dı’az R (2008) Chitosan coating surface properties as affected by plasticizer, surfactant and polymer concentrations in relation to the surface properties of tomato and carrot. Food Hydrocoll 22:1452–1459

    CAS  Article  Google Scholar 

  • Chen RH, Hwa HD (1996) Effect of molecular weight of chitosan with the same degree of deacetylation on the thermal, mechanical, and permeability properties of the prepared membrane. Carbohydr Polym 29:353–358

    CAS  Article  Google Scholar 

  • Chien PJ, Sheu F, Huang WT, Su MS (2007) Effect of molecular weight of chitosans on their antioxidative activities in apple juice. Food Chem 102:1192–1198

    CAS  Article  Google Scholar 

  • Coma V (2008) Bioactive chitosan-based substances and films, In: R. Jayakumar, M. Prabaharan (Eds.), Current research and development on chitin and chitosan in biomaterials science. Trivandrum: Research Signpost 1:21–51

  • Costa-Júnior ES, Mansur HS (2008) Preparação e caracterização de blendas de quitosana/poli (álcool vinílico) reticuladas quimicamente com glutaraldeído para aplicação em engenharia de tecido. Quím Nov. 31:1460–1466

  • Costa-Júnior ES, Barbosa-Stancioli EF, Mansur AAP, Vasconcelos WL, Mansur HS (2009) Preparation and characterization of chitosan/poly (vinyl alcohol) chemically crosslinked blends for biomedical applications. Carbohydr Polym 76:472–481

    Article  Google Scholar 

  • Dutta PK, Tripathi S, Mehrotra GK, Dutta J (2009) Perspectives for chitosan based antimicrobial films in food applications. Food Chem 114:1173–1182

    CAS  Article  Google Scholar 

  • Hafsa J, Smach MA, Ben Khedher MR, Charfeddine B, Limem K, Majdoub H, Rouatbi S (2016) Physical, antioxidant and antimicrobial properties of chitosan films containing Eucalyptus globules essential oil. LWT Food Sci Technol 68:356–364

    CAS  Article  Google Scholar 

  • Hajji S, Younes I, Ghorbel-Bellaaj O, Hajji R, Rinaudo M, Nasri M (2014) Structural differences between chitin and chitosan extracted from three different marine sources. Int J Biol Macromol 65:298–306

    CAS  Article  Google Scholar 

  • Hernandez-Munoz P, Almenar E, Valle VD, Velez D, Gavara R (2008) Effect of chitosan coating combined with postharvest calcium treatment on strawberry (Fragaria-ananassa) quality during refrigerated storage. Food Chem 110:428–435

    CAS  Article  Google Scholar 

  • Iwata K, Ishizaki S, Handa A, Tanaka M (2000) Preparation and characterization of edible films from fish water-soluble proteins. Fish Sci 66:372–378

    CAS  Article  Google Scholar 

  • Jayakumar R, Prabaharan M, Nair SV, Tokura S, Tamura H, Selvamurugan N (2010) Novel carboxymethyl derivatives of chitin and chitosan materials and their biomedical applications. Prog Mater Sci 55:675–709

    CAS  Article  Google Scholar 

  • Jiang Y, Li Y, Chai Z, Leng X (2010) Study of the physical properties of whey protein isolate and gelatin composite films. J Agric Food Chem 58:5100–5108

    CAS  Article  Google Scholar 

  • Jirukkakul N (2013) A study of Mu Yor sausage wraps using chitosan films incorporating garlic oil, lemon grass oil and galangal oil. Int Food Res J 20:1199–1204

    CAS  Google Scholar 

  • Jridi M, Hajji S, Ben Ayed H, Lassoued I, Mbarek A, Kammoun M, Souissi N, Nasri M (2014) Physical, structural, antioxidant and antimicrobial properties of gelatin–chitosan composite edible films. Inter J Biol Macromol 67:373–379

    CAS  Article  Google Scholar 

  • Kanatt SR, Rao MS, Chawla SP, Sharma A (2012) Active chitosan–polyvinyl alcohol films with natural extracts. Food Hydrocoll 29:290–297

    CAS  Article  Google Scholar 

  • Koleva II, Van Beek TA, Linssen JPH, De Groot A, Evstatieva LN (2002) Screening of plant extracts for antioxidant activity: a comparative study on three testing methods. Phytochem Anal 13:8–17

    CAS  Article  Google Scholar 

  • Kumar NHMP, Prabhakar MN, Venkata PC, Madhusudhan RK, Ashok KR, Chowdoji RK, Subha MCS (2010) Compatibility studies of chitosan/ PVA blend in 2% aqueous acetic acid solution at 30 °C. Carbohydr Polym 82:251–255

    CAS  Article  Google Scholar 

  • Lewandowska K (2009) Miscibility and thermal stability of poly (vinyl alcohol)/chitosan mixtures. Thermochim Acta 493:42–48

    CAS  Article  Google Scholar 

  • Limpan N, Prodpran T, Benjakul S, Prasarpran S (2012) Influences of degree of hydrolysis and molecular weight of poly (vinyl alcohol) (PVA) on properties of fish myofibrillar protein/PVA blend films. Food Hydrocoll 29:226–233

    CAS  Article  Google Scholar 

  • Mangala E, Suresh Kumar T, Baskar S, Panduranga Rao K (2003) Development of chitosan/ poly (vinyl alcohol) blend membranes as burn dressings. Trends Biomater Artif Organs 17:34–40

    Google Scholar 

  • Parparita E, Cheaburu CN, Vasile C (2010) Morphological, thermal and rheological characterization of polyvinyl alcohol/chitosan blends. Cell Chem Technol 46:571–581

    Google Scholar 

  • Pitak N, Rakshit SK (2011) Physical and antimicrobial properties of banana flour/chitosan biodegradable and self sealing films used for preserving fresh-cut vegetables. LWT Food Sci Technol 44:2310–2315

    CAS  Article  Google Scholar 

  • Qin C, Li H, Xiao Q, Liu Y, Zhu J, Du Y (2006) Water-solubility of chitosan and its antimicrobial activity. Carbohydr Polym 63:367–374

    CAS  Article  Google Scholar 

  • Qiu X, Chen S, Liu G, Yang Q (2014) Quality enhancement in the Japanese sea bass (Lateolabrax japonicas) fillets stored at 4 °C by chitosan coating incorporated with citric acid or licorice extract. Food Chem 162:156–160

    CAS  Article  Google Scholar 

  • Ramezani Z, Zarei M, Raminnejad N (2015) Comparing the effectiveness of chitosan and nanochitosan coatings on the quality of refrigerated silver carp fillets. Food Control 51:43–48

    CAS  Article  Google Scholar 

  • Rhim JW, Gennadios A, Weller CL, Cezeirat C, Hanna MA (1998) Soy protein-dialdehyde starch film. Lebensm Wiss Technol 32:129–133

    Article  Google Scholar 

  • Sagoo S, Board R, Roller S (2002) Chitosan inhibits growth of spoilage microorganisms in chilled pork products. Food Microbiol 19:175–182

    CAS  Article  Google Scholar 

  • Sathivel S, Liu Q, Huang J, Prinyawiwatkul W (2007) The influence of chitosan glazing on the quality of skinless pink salmon (Oncorhynchus gorbuscha) fillets during frozen storage. J Food Eng 83:366–373

    CAS  Article  Google Scholar 

  • Siripatrawan U, Harte BR (2010) Physical properties and antioxidant activity of an active film from chitosan incorporated with green tea extract. Food Hydrocoll 24:770–775

    CAS  Article  Google Scholar 

  • Srinivasa PC, Tharanathan RN (2007) Chitin/chitosan-safe, ecofriendly packaging materials with multiple potential uses. Food Rev Int 23:53–72

    CAS  Article  Google Scholar 

  • Tan YM, Lim SH, Tay BY, Lee MW, Thian ES (2015) Functional chitosan-based grapefruit seed extract composite films for applications in food packaging technology. Mater Res Bull 69:142–146

    CAS  Article  Google Scholar 

  • Tripathi S, Mehrotra GK, Dutta PK (2010) Preparation and physicochemical evaluation of chitosan/poly (vinyl alcohol)/ pectin ternary film for food-packaging applications. Carbohydr Polym 79:711–716

    CAS  Article  Google Scholar 

  • Vu KD, Hollingsworth RG, Leroux E, Salmieri S, Lacroix M (2011) Development of edible bioactive coating based on modified chitosan for increasing the shelf life of strawberries. Food Res Int 44:198–203

    CAS  Article  Google Scholar 

  • Wang Q, Du Y, Fan L (2005) Properties of chitosan/ poly (vinyl alcohol) films for drug-controlled release. J Appl Polym Sci 96:808–813

    CAS  Article  Google Scholar 

  • Wang Y, Liu L, Zhou J, Ruan X, Lin J, Fu L (2014) Effect of chitosan nanoparticle coatings on the quality changes of postharvest whiteleg shrimp, Litopenaeus vannamei, during storage at 4 °C. Food Bioprocess Technol 8:907–915

    Article  Google Scholar 

  • Wu SJ (2014) Effect of chitosan-based edible coating on preservation of white shrimp during partially frozen storage. Int J Biol Macromol 65:325–328

    CAS  Article  Google Scholar 

  • Xing Y, Li X, Xu Q, Jiang Y, Yun J, Li W (2010) Effects of chitosan-based coating and modified atmosphere packaging (MAP) on browning and shelf life of fresh-cut lotus root (Nelumbo nucifera Gaerth). Innovative Food Sci Emerg Technol 11:684–689

    CAS  Article  Google Scholar 

  • Yen M, Yang J, Mau J (2008) Antioxidant properties of chitosan from crab shells. Carbohydr Polym 74:840–844

    CAS  Article  Google Scholar 

  • Yildirim A, Mavi A, Kara AA (2001) Determination of antioxidant and antimicrobial activities of Rumex crispus L. extracts. J Agric Food Chem 49:4083–4089

    CAS  Article  Google Scholar 

  • Yu Q, Song Y, Shi X, Xu C, Bin Y (2011) Preparation and properties of chitosan derivative/poly (vinyl alcohol) blend film crosslinked with glutaraldehyde. Carbohydr Polym 84:465–470

    CAS  Article  Google Scholar 

Download references

Acknowledgment

This work was funded by the Ministry of Higher Education and Scientific Research, Tunisia.

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Affiliations

  1. University of Sfax- National School of Engineering of Sfax- Laboratory of Enzyme Engineering and Microbiology, BP 1173, 3038, Sfax, Tunisia

    Sawssen Hajji, Mourad Jridi, Hana Maalej, Kemel Jellouli & Moncef Nasri

  2. University of Sfax – Faculty of Science of Sfax-LMSE, BP 802, 3018, Sfax, Tunisia

    Achraf Chaker & Sami Boufi

Authors
  1. Sawssen Hajji
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  2. Achraf Chaker
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  3. Mourad Jridi
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  4. Hana Maalej
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  5. Kemel Jellouli
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  6. Sami Boufi
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  7. Moncef Nasri
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Correspondence to Sawssen Hajji.

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Responsible editor: Santiago V. Luis

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Hajji, S., Chaker, A., Jridi, M. et al. Structural analysis, and antioxidant and antibacterial properties of chitosan-poly (vinyl alcohol) biodegradable films. Environ Sci Pollut Res 23, 15310–15320 (2016). https://doi.org/10.1007/s11356-016-6699-9

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  • DOI: https://doi.org/10.1007/s11356-016-6699-9

Keywords

  • Chitosan/PVA films
  • Mechanical analysis
  • Antioxidant and antibacterial activities