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Effects of glyphosate on hepatic tissue evaluating melanomacrophages and erythrocytes responses in neotropical anuran Leptodactylus latinasus

Environmental Science and Pollution Research volume 23, pages 9852–9861 (2016)Cite this article

Abstract

Glyphosate (GLY) is the most used herbicide worldwide and its effects on anurans are well known. Pollutants can cause physiological and morphological effects. Therefore, this study evaluated the effects of GLY on hepatic melanomacrophages as a response to environmental stressors. Three treatments were exposed to different concentrations of pure GLY (100, 1000, and 10,000 μg g−1, respectively), and there was also a control group. After the experimental time, liver and blood were analyzed. Melanomacrophages (MMCs) were located between the hepatocyte cordons, close to sinusoids. GLY increased the melanin area in MMCs of Leptodactylus latinasus exposed since lowest concentration until highest concentration. GLY also changed the occurrence of hepatic catabolism pigments into melanomacrophages and erythrocyte nuclear abnormalities; therefore, it can interfere with the hepatic metabolism. In conclusion, GLY promotes alterations in the hepatic tissue and erythrocyte nuclear abnormalities. Furthermore, MMCs may be useful as morphological responses of GLY effects.

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Acknowledgments

We are grateful to Gabriela Baroni Leite for helping with the histological procedures, Dr. Fernando Ferrari for help in statistical analysis, and GLEBA S.A. for providing pure glyphosate. This study was supported by CONICET (PIP 0723), FAPESP (Process 2013/02067-5), and CNPq (Grant 306123/2012-6). JMPI was supported by Universidad Nacional de San Luis (Res. No. 109/11 CS), while LFB was supported by a post-doctoral fellowship (FAPESP 2014/00946-4).

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  1. PROICO 9401, Área Zoología, Facultad de Química, Bioquímica y Farmacia, Universidad Nacional de San Luis, Chacabuco 917, San Luis, 5700, Argentina

    Juan Manuel Pérez-Iglesias, Liliana Moreno, Susana Tripole & Guillermo Sebastián Natale

  2. Department of Biology, São Paulo State University (UNESP), IBILCE, Rua Cristóvão Colombo, 2265, Jardim Nazareth, São José do Rio Preto, 15054-000, São Paulo, Brazil

    Lilian Franco-Belussi & Classius de Oliveira

  3. Centro de Investigaciones del Medio Ambiente, Departamento de Química, Facultad de Ciencias Exactas, Universidad Nacional de La Plata, CONICET, La Plata, Argentina

    Juan Manuel Pérez-Iglesias & Guillermo Sebastián Natale

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  1. Juan Manuel Pérez-Iglesias
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Histopathologic lesion in liver of L. latinasus after exposure of glyphosate. (XLSX 35 kb)

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Pérez-Iglesias, J.M., Franco-Belussi, L., Moreno, L. et al. Effects of glyphosate on hepatic tissue evaluating melanomacrophages and erythrocytes responses in neotropical anuran Leptodactylus latinasus . Environ Sci Pollut Res 23, 9852–9861 (2016). https://doi.org/10.1007/s11356-016-6153-z

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Keywords

  • Glyphosate
  • Melanomacrophages
  • Melanin
  • Hemosiderin
  • Morphological responses
  • Genotoxic effects