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Effects of an environmentally relevant concentration of diuron on oyster genitors during gametogenesis: responses of early molecular and cellular markers and physiological impacts

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Abstract

Genitors of the Pacific oyster Crassostrea gigas were submitted during gametogenesis to a short pulse exposure to the herbicide diuron at a realistic environmental concentration. Histological analysis showed no effect of diuron on gametogenesis course, sex ratio and reproductive effort. A non-significant increase in testosterone and progesterone levels was observed in genitors exposed to the herbicide. At cell level, diuron exposure was shown to modulate the phagocytic activity of circulating hemocytes. The results of a transcriptional analysis showed that diuron affected the expression of genes belonging to functions known to play a major role during oyster gametogenesis such as gene transcription regulation, DNA replication and repair, DNA methylation and cytokinesis. Taking into account the results we previously obtained on the same genitors, this study showed a negative effect of diuron on oyster reproduction by inducing both structural and functional modifications of the DNA.

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Acknowledgments

This work was funded by the French National Research Agency (ANR-CESA-01601) and the French Research Institute for Sea Exploration. The authors are grateful to Béatrice Adeline (histological approaches) for her punctual technical assistance.

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Authors and Affiliations

  1. Laboratoire d’Ecotoxicologie, Ifremer, Rue de l’Ile d’Yeu, BP21105, 44311, Nantes cedex 03, France

    F. Akcha, A. Barranger, D. Menard, A. Szczybelski, J. Rouxel & T. Burgeot

  2. Ifremer, UMR 5244, IHPE Interaction Host Pathogen Environment, UPVD, CNRS, Université de Montpellier, CC 80, F-34095, Montpellier, France

    E. Bachère, P. Alonso & R. Rondon Sallan

  3. Unité Biologie des organismes et écosystèmes aquatiques (BOREA, UMR 7208), Université de Caen Normandie, Sorbonne Universités, Muséum national d’Histoire naturelle, Université Pierre et Marie Curie, CNRS, IRD, 57 rue Cuvier, 75005, Paris, France

    C. Heude Berthelin

  4. Acobiom, 1682 rue de la Valsière, CS 77394 Cap Delta Biopole Euromédecine II, 34184, Montpellier Cedex 04, France

    D. Piquemal

  5. IDAEA-CSIC, Environmental Chemistry Department, C/ Jordi Girona, 1808034, Barcelona, Spain

    G. Dimastrogiovanni & C. Porte

  6. Ifremer, Laboratoire de Génétique et Pathologies, Rue de Mus de Loup, La Tremblade, 17390, France

    A. Benabdelmouna

  7. Laboratoire des Sciences de l’Environnement Marin (LEMAR), Institut Universitaire Européen de la Mer (IUEM), UBO/CNRS/IRD/IFREMER, rue Dumont d’Urville, technopôle, Brest-Iroise, 29280, Plouzané, France

    M. Auffret

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  1. F. Akcha
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  2. A. Barranger
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Correspondence to F. Akcha.

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Akcha, F., Barranger, A., Bachère, E. et al. Effects of an environmentally relevant concentration of diuron on oyster genitors during gametogenesis: responses of early molecular and cellular markers and physiological impacts. Environ Sci Pollut Res 23, 8008–8020 (2016). https://doi.org/10.1007/s11356-015-5969-2

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