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Alleviating effects of exogenous NO on tomato seedlings under combined Cu and Cd stress

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Abstract

To investigate the effect of NO on the different origin and regulation of oxidative stress of Cu and/or Cd, tomato seedlings were treated with Cu, Cd, or Cu + Cd in a nutrient solution culture system. The main effect of Cu2+ was a significant reduction in root activity and nitrate reductase (NR) activity, which was similar to that under 50 μM Cd treatment, but promoted Cu accumulation. The supply of Cu under Cd treatment decreased Cd concentration, while not altered Cu concentration by contrast with Cu treatment, which is suggestive of a replacement of Cu2+ with Cd2+ and effective decrease in the boiotoxicity of 50 μM Cd2+ to tomato seedlings. However, NO alleviated the restriction to NR activity significantly and made the biomass of tomato seedlings recover under Cd treatment, and also increased root activity under Cu and Cu + Cd treatment. Exogenous NO markedly reduced the absorption and transportation of Cu but did not obviously change the translocation of Cd to the aboveground parts under Cu + Cd treatment. Both metals induced lipid peroxidation via the decreasing activation of antioxidant enzymes. The antioxidant enzyme system worked differently under Cu, Cd, or Cu + Cd stress. The activities of peroxidase (POD) and catalase (CAT) were higher under single Cd stress than under the control. Meanwhile, Cu + Cd treatment decreased the activities of POD, superoxide dismutase (SOD), and ascorbic acid peroxidase (APX). Exogenous NO increased POD and SOD activities in the leaves and roots, and CAT activity in the roots under combined Cu and Cd stress. These results suggest that a different response and regulation mechanism that involves exogenous NO is present in tomato seedlings under Cu and Cd stress.

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Authors and Affiliations

  1. National Engineering Laboratory for Efficient Utilization of Soil and Fertilizer Resources, College of Resources and Environment, Shandong Agricultural University, Tai’an, China

    Yi-jun Wang & Xiu-min Cui

  2. College of Life Sciences, Shandong Agricultural University, Tai’an, China

    Yu-Xiu Dong

  3. Department of Landscape Engineering, Heze University, Heze, China

    Juan Wang

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  1. Yi-jun Wang
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  2. Yu-Xiu Dong
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  3. Juan Wang
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  4. Xiu-min Cui
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Correspondence to Xiu-min Cui.

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Wang, Yj., Dong, YX., Wang, J. et al. Alleviating effects of exogenous NO on tomato seedlings under combined Cu and Cd stress. Environ Sci Pollut Res 23, 4826–4836 (2016). https://doi.org/10.1007/s11356-015-5525-0

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