Skip to main content
Springer Nature Link
Log in

Phytosynthesis of silver nanoparticles using the leaves extract of Ficus talboti king and evaluation of antioxidant and antibacterial activities

  • Research Article
  • Published:
Environmental Science and Pollution Research Aims and scope Submit manuscript

Abstract

The present study, the synthesis of silver nanoparticles (AgNPs) at 90 °C temperature using an aqueous extract from Ficus talboti leaf and the antioxidant and antibacterial activities of the AgNPs obtained. The devised method is simple and cost-effective, and it produces spherical AgNPs of size 11.9 ± 2.3 nm. The synthesized AgNPs was characterized as UV–vis spectrum and obtain a peak at 438 nm. The phytochemical study result shows that the secondary metabolites such as alkaloids, saponins, phenolic compounds, tannin, flavonoids, phytosterol, and glycosides may be responsible for reducing as well as capping silver ions into AgNPs. Transmission electron microscopic (TEM) studies of the particles revealed a dominance of spherical particle AgNPs. The face centered cubic structure of the AgNPs was confirmed by X-ray diffraction (XRD) peaks at 111°, 200°, 220°, and 311°; SAED patterns confirms the plane of silver nanoparticle planes with clear circular spots on the selected area electron diffraction (SAED). Elemental analysis was done by energy dispersive X-ray analysis (EDX). In addition, this study evaluated the in vitro antioxidant and antibacterial properties of the biosynthesized AgNPs that were found to be significant.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Subscribe and save

Springer+
from $39.99 /Month
  • Starting from 10 chapters or articles per month
  • Access and download chapters and articles from more than 300k books and 2,500 journals
  • Cancel anytime
View plans

Buy Now

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6

Similar content being viewed by others

Explore related subjects

Discover the latest articles, books and news in related subjects, suggested using machine learning.

References

  • Ankamwar B, Chaudhary M, Murali S (2005a) Gold nanotriangles biologically synthesized using tamarind leaf extract and potential application in vapor sensing. Synth React Inorg Met Org Nanomet Chem 35:19–26

    Article  CAS  Google Scholar 

  • Ankamwar B, Chinmay D, Absar A, Murali S (2005b) Biosynthesis of gold and silver nanoparticles using Emblica officinalis fruit extract, their phase transfer and transmetallation in an organic solution. J Nanosci Nanotechnol 10:1665–1671

    Article  Google Scholar 

  • Arangasamy L, Munusamy V (2008) Tapping the unexploited plant resources for the synthesis of silver nanoparticles. African J Biot 7:3162–3165

    CAS  Google Scholar 

  • Arunachalam K, Parimelazhagan T (2014) Evaluation of nutritional composition and antioxidant properties of underutilized Ficus talboti king fruit for nutraceuticals and food supplements. J Food Sci Tech. doi:10.1007/s13197-012-0647-6

    Google Scholar 

  • Blois MS (1958) Antioxidant determination by use of stable free radicals. Nature 181:1199–1200

    Article  CAS  Google Scholar 

  • Chandran SP, Chaudhary M, Pasricha R, Ahmad R, Sastry M (2006) Synthesis of gold nanotriangles and silver nanoparticles using Aloe vera plant extract. Biotechnol Prog 22:577–583

    Article  CAS  Google Scholar 

  • Daizy P (2010) Green synthesis of gold and silver nanoparticles using Hibiscus rosasinensis. Phys E 42:1417–1424

    Article  Google Scholar 

  • Das J, Paul Das M, Velusamy P (2013) Sesbania grandiflora leaf extract mediatedgreen synthesis of antibacterial silver nanoparticles against selected humanpathogens. Spectrochim Acta A Mol Biomol Spectrosc 104:265–270

    Article  CAS  Google Scholar 

  • Dubay M, Bhadauria S, Kushwah BS (2009) Green synthesis of nanosilver particles from extract of Eucalyptus hybrida (safeda) leaf. Dig J Nano-mater Biostruct Dig J Nanomater Biostruct 4:537–543

    Google Scholar 

  • Dwivedi AD, Gopal K (2010) Biosynthesis of silver and gold nanoparticles using Chenopodium album leaf extract. Physicochem Eng Aspects 369:27–33

    Article  CAS  Google Scholar 

  • Geethalakshmi R, Sarada DVSL (2012) Gold and silver nanoparticles from Trianthema decandra: synthesis, characterization, and antimicrobial properties. Int J Nanomed 7:5375–5384

    Article  CAS  Google Scholar 

  • Ghorbani HR, Safekordi AA, Attar H, Sorkhabadi SMR (2011) Biological and non-biological methods for silver nanoparticles synthesis. Chem Biochem Eng Q 25:317–326

    CAS  Google Scholar 

  • Ghosh S, Patil S, Ahire M, Kitture R, Kale S, Pardesi K, Cameotra SS, Bellare J, Dhavale DD, Jabqunde A, Chopade BA (2012) Synthesis of silver nanoparticles using Dioscorea bulbifera tuber extract and evaluation of its synergistic potential in combination with antimicrobial agents. Int J Nanomed 7:483–496

    CAS  Google Scholar 

  • Gopinath V, Mubarak AD, Priyadarshini S, Meera PN, Thajuddin N, Velusamy P (2012) Biosynthesis of silver nanoparticles from Tribulus terrestris and its antimicrobial activity: a novel biological approach. Colloids Surf B: Biointerfaces 96:69–74

    Article  CAS  Google Scholar 

  • Iravani S (2011) Green synthesis of metal nanoparticles using plants. Green Chem 13:2638–2650

    Article  CAS  Google Scholar 

  • Kannan N, Mukunthan K, Balaji S (2011) A comparative study of morphology, reactivity and stability of synthesized silver nanoparticles using Bacillus subtilis and Catharanthus roseus (L.) G. Don. Colloids Surf B: Biointerfaces 86:378–383

    Article  CAS  Google Scholar 

  • Khandelwal N, Singh A, Jain D, Upadhyay MK, Verma HN (2007) Green synthesis of silver nanoparticles using Argimone maxicana leaf extract and evaluation of their activity. Digest J Nanomat Biost 5:483–489

    Google Scholar 

  • Kouvaris P, Delimitis A, Zaspalis V, Papadopoulos D, Tsipas SA, Michailidis N (2012) Green synthesis and characterization of silver nanoparticles produced using Arbutus unedo leaf extract. Mat Lett 76:18–20

    Article  CAS  Google Scholar 

  • Krishnaraj C, Jagan EG, Rajasekar S, Selvakumar P, Kalaichelvan PT, Mohan N (2010) Synthesis of silver nanoparticles using Acalypha indica leaf extracts and its antibacterial activity against waterborne pathogens. Colloids Surf B: Biointerfaces 76:50–56

    Article  CAS  Google Scholar 

  • Logeswari P, Silambarasan S, Abraham J (2015) Synthesis of silver nanoparticles using plants extract and analysis of their antimicrobial property. J Saudi Chem Soc 19:311–317

    Article  Google Scholar 

  • Loo YY, Chieng BW, Nishibuchi M, Radu S (2012) Synthesis of silver nanoparticles by using tea leaf extract from Camellia Sinensis. Int J Nanomed 7:4263–4267

    CAS  Google Scholar 

  • Morgan JF, Klucas RV, Grayer RJ, Abian J, Becan M (1997) Complexes of iron with phenolic compounds from soybean nodules and other legume tissues: prooxidant and antioxidant properties. Free Rad Biol Med 22:861–870

    Article  Google Scholar 

  • Narayanan KB, Sakthivel N (2010) Biological synthesis of metal nanoparticles bymicrobes. Adv Colloid Interface Sci 156:1–13

    Article  CAS  Google Scholar 

  • Perez C, Pauli M, Bazerque P (1990) An antibiotic assay by the agar-well diffusion method. Acta Biol Med Exp 15:113–115

    Google Scholar 

  • Ponarulselvam S, Panneerselvam C, Murugan K, Aarthi N, Kalimuthu K, Thangamani S (2012) Synthesis of silver nanoparticles using leaves of Catharanthus roseus Linn. G. Don and their antiplasmodial activities. Asian Pacific J Trop Biomed 2:574–80

    Article  CAS  Google Scholar 

  • Pullaiah T (2006) Encyclopaedia of world medicinal plants. Vol.1, Regency Publications, NewDelhi, ISBN: 81-89233-42-4, pp: 223

  • Raaman N (2006) Phytochemical techniques. New India publishing agency. Jai Bharat Printing Press, New Delhi, pp 19–22

    Google Scholar 

  • Ramya M, Subapriya MS (2012) Green synthesis of silver nanoparticles. Int J Pharm Med Biol Sci 1:54–61

    CAS  Google Scholar 

  • Re R, Pellegrini N, Proteggente A, Pannala A, Yang M, Rice-Evans C (1999) Antioxidant activity applying an improved ABTS radical cation decolorization assay, free radic. Biol Med 26:1231–1237

    CAS  Google Scholar 

  • Rodríguez-León E, I˜niguez-Palomares R, Navarro RE, Herrera-Urbina R, Tánori J, I˜niguez-Palomares C, Maldonado A (2013) Synthesis of silver nanoparticles using reducing agents obtained from natural sources (Rumex hymenosepalus extracts). Nanoscale Res Lett 8:318–326

    Article  Google Scholar 

  • Sathishkumar G, Gobinath C, Karpagam K, Hemamalini V, Premkumar K, Sivaramakrishnan S (2012) Phyto-synthesis of silver nanoscale particles using Morinda citrifolia L: and its inhibitory activity against human pathogens. Colloids Surf B: Biointerfaces 95:235–240

    Article  CAS  Google Scholar 

  • Saxena A, Tripathi RM, Singh RP (2010) Biological synthesis of silver nanoparticles by using onion (Allium cepa) extract and their antibacterial activity. Digest J Nanomat Biostruct 5:427–432

    Google Scholar 

  • Song JY, Kim BS (2009) Rapid biological synthesis of silver nanoparticles using plant leaf extracts. Bioprocess Biosyst Eng 32:79

    Article  Google Scholar 

  • Subba Rao Y, Kotakadi VS, Prasad TNVKV, Reddy AV, Sai Gopal DVR (2013) Green synthesis and spectral characterization of silver nanoparticles from Lakshmi tulasi (Ocimum sanctum) leaf extract. Spectrochim Acta A Mol Biomol Spectrosc 103:156–159

    Article  CAS  Google Scholar 

  • Subramanian V, Suja S (2012) Green synthesis of silver nanoparticles using Coleus amboinicus Lour, antioxidant activity and in vitro cytotoxicity against Ehrlich’s Ascite carcinoma. J Pharm Res 5:1268–1272

    Google Scholar 

  • Sun Y, Xia Y (2002) Shape-controlled synthesis of gold and silver nanoparticles. Science 298:2176–2179

    Article  CAS  Google Scholar 

  • Tripathi RM, Nishant K, Archana S, Priti S, Shrivastav BR (2013) Catalytic activity of biogenic silver nanoparticles synthesized by Ficus panda leaf extract. J Mol Cat B: Enzymatic 96:75–80

    Article  CAS  Google Scholar 

  • Vijayakumar M, Priya K, Nancy FT, Noorlidah A, Ahmed ABA (2013) Biosynthesis, characterization and antibacterial effect of plant-mediated silver nanoparticles using Artemisia nilagirica. Ind Crop Prod 41:235–240

    Article  CAS  Google Scholar 

  • Vijayaraghavan K, Nalini S, Prakash NU, Madhan kumar D (2012) One step green synthesis of silver nano/microparticles using extracts of Trachyspermum ammi and Papaver somniferum. Colloids Surf B: Biointerfaces 94:114–117

    Article  CAS  Google Scholar 

Download references

Acknowledgments

The first author gratefully acknowledged to Programa Nacional de Pós-doutorado/CAPES (PNPD/CAPES 2014) Brazil for giving that the postdoctoral fellowship. We thank Dr. K. Kadirvelu DRDO-BU Center for Life Sciences and Dr. Anuradha M. Ashok Material Science, PSG Institute of Advanced Studies, Coimbatore for providing all necessary laboratory facilities. We are grateful to Dr. M. Ilanchelian, Mr. P. Manivel Department of Chemistry, Dr. D. Nataraj Department of Physics and Dr. S. R. Prabakaran, Department of Biotechnology, Bharathiar University, Coimbatore for their immense support during the course of the research work.

Conflict of interest

Authors are in the alphabetic order with the exception of the corresponding author. They declare no competing conflict of interest. BS (P.G student) and PS.S (Research scholar) are students from Department of Botany, Bharathiar University, Coimbatore, Tamil Nadu, and the Bharathiar University is a governmental support educational institute. TP (Professor) is working as a faculty in Bharathiar University, Department of Botany; KA is working as a Postdoctoral Fellow in Department of Basic Science in Health, Faculty of Medicine, Federal University of Matto Gross, Cuiaba, Brazil.

Author information

Author notes

  1. T. Parimelazhagan

    Present address: Department of Pharmaceutical Sciences, College of Pharmacy, University of Hawaii, Hilo, 96720, USA

Authors and Affiliations

  1. Department of Basic Sciences in Health, Faculty of Medical Sciences, Federal University of Mato Grosso (UFMT), Av. Fernando Correa da Costa, no. 2367, Coxipó, Cuiabá, Mato Grosso, Zip Code: 78060-900, Brazil

    K. Arunachalam

  2. Bioprospecting Laboratory, Department of Botany, Bharathiar University, Coimbatore, 641 046, Tamil Nadu, India

    B. Shanmuganathan, P. S. Sreeja & T. Parimelazhagan

Authors
  1. K. Arunachalam
    View author publications

    Search author on:PubMed Google Scholar

  2. B. Shanmuganathan
    View author publications

    Search author on:PubMed Google Scholar

  3. P. S. Sreeja
    View author publications

    Search author on:PubMed Google Scholar

  4. T. Parimelazhagan
    View author publications

    Search author on:PubMed Google Scholar

Corresponding author

Correspondence to T. Parimelazhagan.

Additional information

Responsible editor: Philippe Garrigues

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Arunachalam, K., Shanmuganathan, B., Sreeja, P.S. et al. Phytosynthesis of silver nanoparticles using the leaves extract of Ficus talboti king and evaluation of antioxidant and antibacterial activities. Environ Sci Pollut Res 22, 18066–18075 (2015). https://doi.org/10.1007/s11356-015-4992-7

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11356-015-4992-7

Keywords