Abstract
The degradation of the marine environment is a subject of concern for the European authorities primarily because of its contamination by hydrocarbons. The traditional methods (ISO 11348 standard) of general toxicity assessment are unsuitable in a context of in situ monitoring, such as seaports or bathing zones. Consequently, to address this issue, bacterial biosensors appear to be pertinent tools. This article presents the design of an innovative bioluminescent biosensor dedicated to in situ toxicity monitoring. This biosensor is based on the entrapment of the wild marine bioluminescent bacterial strain Aliivibrio fischeri ATCC® 49387™ in an agarose matrix within a disposable card. A pre-study was needed to select the most biological parameters. In particular, the regenerating medium’s composition and the hydrogel concentration needed for the bacterial entrapment (mechanical resistance) were optimized. Based on these data, the ability of the bacterial reporter to assess the sample toxicity was demonstrated using naphthalene as a chemical model. The biosensor’s results show a lower sensitivity to naphthalene (EC50 = 95 mg/L) compared with the results obtained using the reference method (EC50 = 43 mg/L). With this architecture, the biosensor is an interesting compromise among low maintenance, ease of use, appropriate sensitivity, relatively low cost and the ability to control online toxicity.
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References
Affi M, Solliec C, Legentillomme P et al (2009) Numerical design of a card and related physicochemical phenomena occurring inside agarose-immobilized bacteria: a valuable tool for increasing our knowledge of biosensors. Sens Actuators B Chem 138:310–317
Bulich AA, Isenberg DL (1981) Use of the luminescent bacterial system for the rapid assessment of aquatic toxicity. ISA Trans 20:29–33
Decision No 2455/2001/EC of the European Parliament and of the Council of 20 November 2001 establishing the list of priority substances in the field of water policy and amending Directive 2000/60/EC
Hao OJ, Shin C-J, Lin C-F et al (1996) Use of microtox tests for screening industrial wastewater toxicity. Water Sci Technol 34:43–50. doi:10.1016/S0273-1223(96)00695-6
ISO 11348 (2007) Water quality—determination of the inhibitory effect of water samples on the light emission of Vibrio fischeri (Luminescent bacteria test).
ISO 11348-2 (2007) Water quality—determination of the inhibitory effect of water samples on the light emission of Vibrio fischeri (Luminescent bacteria test)—part 2: method using liquid-dried bacteria
Jennings VLK, Rayner-Brandes MH, Bird DJ (2001) Assessing chemical toxicity with the bioluminescent photobacterium (Vibrio fischeri): a comparison of three commercial systems. Water Res 35:3448–3456. doi:10.1016/S0043-1354(01)00067-7
Jouanneau S (2011) Développement d’une méthodologie d’analyse de données par arbre de décision appliquée à un biocapteur multicanal. University of Nantes
Jouanneau S, Durand MJ, Courcoux P, Blusseau T, Thouand G (2011) Improvement of the identification of four heavy metals in environmental samples by using predictive decision tree models coupled with a set of five bioluminescent bacteria. Environ Sci Technol 45:2925–2931
Kokkali V, van Delft W (2014) Overview of commercially available bioassays for assessing chemical toxicity in aqueous samples. TrAC, Trends Anal Chem 61:133–155. doi:10.1016/j.trac.2014.08.001
Milton DL (2006) Quorum sensing in vibrios: complexity for diversification. Int J Med Microbiol 296:61–71. doi:10.1016/j.ijmm.2006.01.044
Yan L, Allen MS, Simpson ML et al (2007) Direct quantification of N-(3-oxo-hexanoyl)-l-homoserine lactone in culture supernatant using a whole-cell bioreporter. J Microbiol Methods 68:40–45. doi:10.1016/j.mimet.2006.06.002
Acknowledgments
The research was funded through CER 2000-2006 Action 15 Grant (section 1), Research Program no. 18035: Ville de La Roche-sur-Yon, Conseil Général de Vendée, Conseil Régional des Pays de la Loire, Ministère Français chargé de la Recherche (DRRT Pays de la Loire).
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Jouanneau, S., Durand-Thouand, MJ. & Thouand, G. Design of a toxicity biosensor based on Aliivibrio fischeri entrapped in a disposable card. Environ Sci Pollut Res 23, 4340–4345 (2016). https://doi.org/10.1007/s11356-015-4942-4
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DOI: https://doi.org/10.1007/s11356-015-4942-4