Environmental Science and Pollution Research

, Volume 23, Issue 5, pp 4207–4217 | Cite as

Functional and structural responses of soil N-cycling microbial communities to the herbicide mesotrione: a dose-effect microcosm approach

  • Olivier Crouzet
  • Franck Poly
  • Frédérique Bonnemoy
  • David Bru
  • Isabelle Batisson
  • Jacques Bohatier
  • Laurent Philippot
  • Clarisse Mallet
Research Article

Abstract

Microbial communities driving the nitrogen cycle contribute to ecosystem services such as crop production and air, soil, and water quality. The responses to herbicide stress of ammonia-oxidizing and ammonia-denitrifying microbial communities were investigated by an analysis of changes in structure-function relationships. Their potential activities, abundances (quantitative PCR), and genetic structure (denaturing gradient gel electrophoresis) were assessed in a microcosm experiment. The application rate (1 × FR, 0.45 μg g−1 soil) of the mesotrione herbicide did not strongly affect soil N-nutrient dynamics or microbial community structure and abundances. Doses of the commercial product Callisto® (10 × FR and 100 × FR) or pure mesotrione (100 × FR) exceeding field rates induced short-term inhibition of nitrification and a lasting stimulation of denitrification. These effects could play a part in the increase in soil ammonium content and decrease in nitrate contents observed in treated soils. These functional impacts were mainly correlated with abundance shifts of ammonia-oxidizing Bacteria (AOB) and Archaea (AOA) or denitrifying bacteria. The sustained restoration of nitrification activity, from day 42 in the 100 × FR-treated soils, was likely promoted by changes in the community size and composition of AOB, which suggests a leading role, rather than AOA, for soil nitrification restoration after herbicide stress. This ecotoxicological community approach provides a nonesuch multiparameter assessment of responses of N-cycling microbial guilds to pesticide stress.

Keywords

Herbicide Soil microcosms Ammonia oxidizers Denitrifiers Microbial ecotoxicology 

Supplementary material

11356_2015_4797_MOESM1_ESM.ppt (904 kb)
Fig. S1(PPT 904 kb)
11356_2015_4797_MOESM2_ESM.ppt (274 kb)
Table S1(PPT 274 kb)

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Copyright information

© Springer-Verlag Berlin Heidelberg 2015

Authors and Affiliations

  • Olivier Crouzet
    • 1
  • Franck Poly
    • 4
    • 5
  • Frédérique Bonnemoy
    • 2
    • 3
  • David Bru
    • 6
  • Isabelle Batisson
    • 2
    • 3
  • Jacques Bohatier
    • 2
    • 3
  • Laurent Philippot
    • 6
  • Clarisse Mallet
    • 2
    • 3
  1. 1.INRA UR 251 PESSAC, Centre Versailles-GrignonVersailles cedexFrance
  2. 2.CNRS UMR 6023 LMGEAubière cedexFrance
  3. 3.Clermont Université, Université Blaise PascalClermont-FerrandFrance
  4. 4.Ecologie MicrobienneINRA USC 1193 - CNRS UMR 5557VilleurbanneFrance
  5. 5.Ecologie MicrobienneUniversité de Lyon, Université Lyon 1VilleurbanneFrance
  6. 6.AgroécologieINRA, UMR 1347Dijon cedexFrance

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