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Possibility of biological control of primocane fruiting raspberry disease caused by Fusarium sambucinum

Environmental Science and Pollution Research volume 22, pages 15656–15662 (2015)Cite this article

Abstract

Biological control agents are a promising alternative to chemical pesticides for plant disease suppression. The main advantage of the natural biocontrol agents, such as antagonistic bacteria compared with chemicals, includes environmental pollution prevention and a decrease of chemical residues in fruits. This study is aimed to evaluate the impact of three Bacillus strains on disease of primocane fruiting raspberry canes caused by Fusarium sambucinum under controlled infection load and uncontrolled environmental factors. Bacillus subtilis, Bacillus licheniformis, and Bacillus amyloliquefaciens were used for biocontrol of plant disease in 2013 and 2014 which differed by environmental conditions. The test suspensions were 105 CFU/ml for each bacterial strain. To estimate the effect of biological agents on Fusarium disease, canes were cut at the end of vegetation, and the area of outer and internal lesions was measured. In addition to antagonistic effect, the strains revealed the ability to induce plant resistance comparable with chitosan-based formulation. Under variable ways of cane treatment by bacterial strains, the more effective were B. subtilis and B. licheniformis demonstrating dual biocontrol effect. However, environmental factors were shown to impact the strain biocontrol ability; changes in air temperature and humidity led to the enhanced activity of B. amyloliquefaciens. For the first time, the possibility of replacing chemicals with environmentally benign biological agents for ecologically safe control of the raspberry primocane fruiting disease was shown.

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Acknowledgments

This research was supported by the grant of Russian Scientific Foundation (project No. 14-16-00101).

Conflict of interest

The authors declare that they have no competing interests.

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Affiliations

  1. Department of Plant Protection, Novosibirsk State Agrarian University, Dobrolubov 160, Novosibirsk, Russia, 630039

    Margarita V. Shternshis, Anatoly A. Belyaev, Nina S. Matchenko, Tatyana V. Shpatova & Anastasya A. Lelyak

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  1. Margarita V. Shternshis
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  2. Anatoly A. Belyaev
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  3. Nina S. Matchenko
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  4. Tatyana V. Shpatova
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  5. Anastasya A. Lelyak
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Correspondence to Margarita V. Shternshis.

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Responsible editor: Philippe Garrigues

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Shternshis, M.V., Belyaev, A.A., Matchenko, N.S. et al. Possibility of biological control of primocane fruiting raspberry disease caused by Fusarium sambucinum . Environ Sci Pollut Res 22, 15656–15662 (2015). https://doi.org/10.1007/s11356-015-4763-5

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  • DOI: https://doi.org/10.1007/s11356-015-4763-5

Keywords

  • Bacillus strains
  • Fungal infection
  • Fusarium sambucinum
  • Induced resistance
  • Plant disease biocontrol
  • Primocane fruiting raspberry