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Simultaneous detection of imidacloprid and parathion by the dual-labeled time-resolved fluoroimmunoassay


A highly sensitive direct dual-labeled time-resolved fluoroimmunoassay (TRFIA) to detect parathion and imidacloprid simultaneously in food and environmental matrices was developed. Europium (Eu3+) and samarium (Sm3+) were used as fluorescent labels by coupling separately with L1-Ab and A1P1-Ab. Under optimal assay conditions, the half-maximal inhibition concentration (IC50) and limit of detection (LOD, IC10) were 10.87 and 0.025 μg/L for parathion and 7.08 and 0.028 μg/L for imidacloprid, respectively. The cross-reactivities (CR) were negligible except for methyl-parathion (42.4 %) and imidaclothiz (103.4 %). The average recoveries of imidacloprid ranged from 78.9 to 104.2 % in water, soil, rice, tomato, and Chinese cabbage with a relative standard deviation (RSD) of 2.4 to 11.6 %, and those of parathion were from 81.5 to 110.9 % with the RSD of 3.2 to 10.5 %. The results of TRFIA for the authentic samples were validated by comparison with gas chromatography (GC) analyses, and satisfactory correlations (parathion: R 2 = 0.9918; imidacloprid: R 2 = 0.9908) were obtained. The results indicate that the dual-labeled TRFIA is convenient and reliable to detect parathion and imidacloprid simultaneously in food and environmental matrices.

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This project was supported by the National Natural Science Foundation of China (31301692) and the Fundamental Research Funds for the Central Universities (KJQN201433 and KYZ201305).

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Correspondence to Minghua Wang.

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Responsible editor: Laura McConnell

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Shi, H., Sheng, E., Feng, L. et al. Simultaneous detection of imidacloprid and parathion by the dual-labeled time-resolved fluoroimmunoassay. Environ Sci Pollut Res 22, 14882–14890 (2015).

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  • Imidacloprid
  • Parathion
  • Europium
  • Samarium
  • Time-resolved fluoroimmunoassay