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Evaluating the efficacy of bioremediating a diesel-contaminated soil using ecotoxicological and bacterial community indices

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Abstract

Diesel represents a common environmental contaminant as a result of operation, storage, and transportation accidents. The bioremediation of diesel in a contaminated soil is seen as an environmentally safe approach to treat contaminated land. The effectiveness of the remediation process is usually assessed by the degradation of the total petroleum hydrocarbon (TPH) concentration, without considering ecotoxicological effects. The aim of this study was to assess the efficacy of two bioremediation strategies in terms of reduction in TPH concentration together with ecotoxicity indices and changes in the bacterial diversity assessed using PCR-denaturing gradient gel electrophoresis (DGGE). The biostimulation strategy resulted in a 90 % reduction in the TPH concentration versus 78 % reduction from the natural attenuation strategy over 12 weeks incubation in a laboratory mesocosm-containing diesel-contaminated soil. In contrast, the reduction in the ecotoxicity resulting from the natural attenuation treatment using the Microtox and earthworm toxicity assays was more than double the reduction resulting from the biostimulation treatment (45 and 20 % reduction, respectively). The biostimulated treatment involved the addition of nitrogen and phosphorus in order to stimulate the microorganisms by creating an optimal C:N:P molar ratio. An increased concentration of ammonium and phosphate was detected in the biostimulated soil compared with the naturally attenuated samples before and after the remediation process. Furthermore, through PCR-DGGE, significant changes in the bacterial community were observed as a consequence of adding the nutrients together with the diesel (biostimulation), resulting in the formation of distinctly different bacterial communities in the soil subjected to the two strategies used in this study. These findings indicate the suitability of both bioremediation approaches in treating hydrocarbon-contaminated soil, particularly biostimulation. Although biostimulation represents a commercially viable bioremediation technology for use in diesel-contaminated soils, further research is required to determine the ecotoxicological impacts of the intervention.

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References

  • Adam G, Duncan HJ (1999) Effect of diesel fuel on growth of selected plant species. Environ Geochem Health 21:353–357

    Article  CAS  Google Scholar 

  • ASTM (2004) Standard test method for assessing the microbial detoxification of chemically contaminated water and soil using a toxicity test with a luminescent marine bacterium. Society for Testing and Materials Standard Guideline, American

    Google Scholar 

  • Atlas RM (1991) Microbial hydrocarbon degradation—bioremediation of oil spills. J Chem Technol Biotechnol 52:149–156

    Article  CAS  Google Scholar 

  • Bento FM, Camargo FAO, Okeke BC, Frankenberger WT (2005) Comparative bioremediation of soils contaminated with diesel oil by natural attenuation, biostimulation and bioaugmentation. Bioresour Technol 96:1049–1055

    Article  CAS  Google Scholar 

  • Callaham MA, Stewart AJ, Alarcon C, McMillen SJ (2002) Effects of earthworm (Eisenia fetida) and wheat (Triticum aestivum) straw additions on selected properties of petroleum-contaminated soils. Environ Toxicol Chem 21:1658–1663

    Article  CAS  Google Scholar 

  • Cornelissen G, Rigterink H, Ferdinandy MMA, Van Noort PCM (1998) Rapidly desorbing fractions of PAHs in contaminated sediments as a predictor of the extent of bioremediation. Environ Sci Technol 32:966–970

    Article  CAS  Google Scholar 

  • Coulon F, Pelletier E, Gourhant L, Delille D (2005) Effects of nutrient and temperature on degradation of petroleum hydrocarbons in contaminated sub-Antarctic soil. Chemosphere 58:1439–1448

    Article  CAS  Google Scholar 

  • Dąbrowska H, Dąbrowski Ł, Biziuk M, Gaca J, Namieśnik J (2003) Solid-phase extraction clean-up of soil and sediment extracts for the determination of various types of pollutants in a single run. J Chromatogr A 1003:29–42

    Article  Google Scholar 

  • Davies NA, Hodson ME, Black S (2003) The influence of time on lead toxicity and bioaccumulation determined by the OECD earthworm toxicity test. Environ Pollut 121:55–61

    Article  CAS  Google Scholar 

  • Di Cello F, Pepi M, Baldi F, Fani R (1997) Molecular characterization of an n-alkane-degrading bacterial community and identification of a new species, Acinetobacter venetianus. Res Microbiol 148:237–249

    Article  Google Scholar 

  • Dorn PB, Salanitro JP (2000) Temporal ecological assessment of oil contaminated soils before and after bioremediation. Chemosphere 40:419–426

    Article  CAS  Google Scholar 

  • Duarte GF, Rosado AS, Seldin L, de Araujo W, van Elsas JD (2001) Analysis of bacterial community structure in sulfurous-oil-containing soils and detection of species carrying dibenzothiophene desulfurization (dsz). Genes Appl Environ Microbiol 67:1052–1062

  • Dutka BJ, Kwan KK (1981) Comparison of three microbial toxicity screening tests with the Microtox test. Bull Environ Contam Toxicol 27:753–757

    Article  CAS  Google Scholar 

  • Evans FF et al (2004) Impact of oil contamination and biostimulation on the diversity of indigenous bacterial communities in soil microcosms. FEMS Microbiol Ecol 49:295–305

    Article  CAS  Google Scholar 

  • Gallego JLR, Loredo J, Llamas JF, Vázquez F, Sánchez J (2001) Bioremediation of diesel-contaminated soils: evaluation of potential in situ techniques by study of bacterial degradation. Biodegradation 12:325–335

    Article  CAS  Google Scholar 

  • Guerin WF, Boyd SA (1992) Differential bioavailability of soil-sorbed naphthalene to 2 bacterial species. Appl Environ Microbiol 58:1142–1152

    CAS  Google Scholar 

  • Hubálek T, Vosáhlová S, Matějů V, Kováčová N, Novotný Č (2007) Ecotoxicity monitoring of hydrocarbon-contaminated soil during bioremediation: a case study. Arch Environ Contam Toxicol 52:1–7

    Article  Google Scholar 

  • ISO (2008) Soil quality—avoidance test for determining the quality of soils and effects of chemicals on behaviour—part 1: test with earthworms (Eisenia fetida and Eisenia andrei). International Organization for Standardization, Paris-France

  • Iwamoto T, Nasu M (2001) Current bioremediation practice and perspective. J Biosci Bioeng 92:1–8

    Article  CAS  Google Scholar 

  • Kamlet MJ, Doherty RM, Veith GD, Taft RW, Abraham MH (1986) Solubility properties in polymers and biological media. 7. An analysis of toxicant properties that influence inhibition of bioluminescence in Photobacterium phosphoreum (the Microtox test). Environ Sci Technol 20:690–695

    Article  CAS  Google Scholar 

  • Kang Y-S, Park W (2010) Protection against diesel oil toxicity by sodium chloride-induced exopolysaccharides in Acinetobacter sp. strain DR1. J Biosci Bioeng 109:118–123

    Article  CAS  Google Scholar 

  • Kvenvolden KA, Cooper CK (2003) Natural seepage of crude oil into the marine environment. Geo-Mar Lett 23:140–146

    Article  CAS  Google Scholar 

  • Lanno R, Wells J, Conder J, Bradham K, Basta N (2004) The bioavailability of chemicals in soil for earthworms. Ecotoxicol Environ Saf 57:39–47

    Article  CAS  Google Scholar 

  • Makadia TH, Adetutu EM, Simons KL, Jardine D, Sheppard PJ, Ball AS (2011) Re-use of remediated soils for the bioremediation of waste oil sludge. J Environ Manag 92:866–871

    Article  CAS  Google Scholar 

  • Mills MA, Bonner JS, McDonald TJ, Page CA, Autenrieth RL (2003) Intrinsic bioremediation of a petroleum-impacted wetland. Mar Pollut Bull 46:887–899

    Article  CAS  Google Scholar 

  • Mills SA, Frankenberger WT Jr (1994) Evaluation of phosphorus sources promoting bioremediation of diesel fuel in soil. Bull Environ Contam Toxicol 53:280–284

    CAS  Google Scholar 

  • Molina-Barahona L, Vega-Loyo L, Guerrero M, Ramírez S, Romero I, Vega-Jarquín C, Albores A (2005) Ecotoxicological evaluation of diesel-contaminated soil before and after a bioremediation process. Environ Toxicol 20:100–109

    Article  CAS  Google Scholar 

  • Mooney TJ, King CK, Wasley J, Andrew NR (2013) Toxicity of diesel contaminated soils to the subantarctic earthworm Microscolex macquariensis. Environ Toxicol Chem 32:370–377

    Article  CAS  Google Scholar 

  • Mos L, Cooper GA, Serben K, Cameron M, Koop BF (2008) Effects of diesel on survival, growth, and gene expression in rainbow trout (Oncorhynchus mykiss). Fry Environm Sci Technol 42:2656–2662

  • Nakatsu C (2007) Soil microbial community analysis using denaturing gradient gel electrophoresis. Soil Sci Soc Am J 71:562–571

    Article  CAS  Google Scholar 

  • OECD (1984) OECD guideline for testing of chemicals “Earthworm, Acute Toxicity Tests” vol 207. The Organisation for Economic Co-operation and Development Paris-France

  • Phillips TM, Liu D, Seech AG, Lee H, Trevors JT (2000) Monitoring bioremediation in creosote-contaminated soils using chemical analysis and toxicity tests. J Ind Microbiol Biotech 24:132–139

    Article  CAS  Google Scholar 

  • Reid BJ, Jones KC, Semple KT (2000) Bioavailability of persistent organic pollutants in soils and sediments—a perspective on mechanisms, consequences and assessment. Environ Pollut 108:103–112

    Article  CAS  Google Scholar 

  • Salanitro JP et al (1997) Crude oil hydrocarbon bioremediation and soil ecotoxicity. Asses Environ Sci Technol 31:1769–1776

    Article  CAS  Google Scholar 

  • Sanders G, Jones KC, Hamilton-Taylor J, Dorr H (1993) Concentrations and deposition fluxes of polynuclear aromatic hydrocarbons and heavy metals in the dated sediments of a rural English lake. Environ Toxicol Chem 12:1567–1581

    Article  CAS  Google Scholar 

  • Seklemova E, Pavlova A, Kovacheva K (2001) Biostimulation-based bioremediation of diesel fuel: field demonstration. Biodegradation 12:311–316

    Article  CAS  Google Scholar 

  • Semple KT, Morriss AWJ, Paton GI (2003) Bioavailability of hydrophobic organic contaminants in soils: fundamental concepts and techniques for analysis. Eur J Soil Sci 54:809–818

    Article  CAS  Google Scholar 

  • Simons KL, Ansar A, Kadali K, Bueti A, Adetutu EM, Ball AS (2012) Investigating the effectiveness of economically sustainable carrier material complexes for marine oil remediation. Bioresour Technol 126:202–207

    Article  CAS  Google Scholar 

  • Straube WL, Nestler CC, Hansen LD, Ringleberg D, Pritchard PH, Jones-Meehan J (2003) Remediation of polyaromatic hydrocarbons (PAHs) through landfarming with biostimulation and bioaugmentation. Acta Biotechnol 23:179–196

    Article  CAS  Google Scholar 

  • Tadesse B, Donaldson JD, Grimes SM (1994) Contaminated and polluted land: a general review of decontamination management and control. J Chem Technol Biotechnol 60:227–240

    Article  CAS  Google Scholar 

  • Tang J, Wang M, Wang F, Sun Q, Zhou Q (2011) Eco-toxicity of petroleum hydrocarbon contaminated soil. J Environ Sci 23:845–851

    Article  CAS  Google Scholar 

  • van Beilen JB, Li Z, Duetz WA, Smits THM, Witholt B (2003) Diversity of alkane hydroxylase systems in the environment Diversité des systèmes alcane hydroxylase dans l'environnement 58:427–440

  • Xia WX, Li JC, Zheng XL, Bi XJ, Shao JL (2006) Enhanced biodegradation of diesel oil in seawater supplemented with nutrients. Eng Life Sci 6:80–85

    Article  CAS  Google Scholar 

  • Xu Y, Lu M (2010) Bioremediation of crude oil-contaminated soil: comparison of different biostimulation and bioaugmentation treatments. J Hazard Mater 183:395–401

    Article  CAS  Google Scholar 

  • Yerushalmi L, Rocheleau S, Cimpoia R, Sarrazin M et al (2003) Enhanced biodegradation of petroleum hydrocarbons in contaminated soil. Bioremediation J 7:37

    Article  CAS  Google Scholar 

  • Yu KSH, Wong AHY, Yau KWY, Wong YS, Tam NFY (2005) Natural attenuation, biostimulation and bioaugmentation on biodegradation of polycyclic aromatic hydrocarbons (PAHs) in mangrove sediments. Mar Pollut Bull 51:1071–1077

    Article  CAS  Google Scholar 

Download references

Acknowledgments

This work was supported by the award of a grant from the Australian Research Council to AS Ball (Grant No. LP110201130).

Compliance with ethical standards

Ethical approval is not required for this research work. This study was performed in accordance with the PC2 Australian Standards at School of Applied Sciences, RMIT University.

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Authors and Affiliations

  1. Centre for Environmental Sustainability and Remediation, School of Applied Sciences, RMIT University, Melbourne, VIC, 3083, Australia

    Leadin Salah Khudur, Esmaeil Shahsavari, Ana F. Miranda, Paul D. Morrison, Dayanthi Nugegoda & Andrew S. Ball

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  1. Leadin Salah Khudur
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  2. Esmaeil Shahsavari
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  3. Ana F. Miranda
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  4. Paul D. Morrison
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  5. Dayanthi Nugegoda
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  6. Andrew S. Ball
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Correspondence to Andrew S. Ball.

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Responsible editor: Cinta Porte

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Khudur, L.S., Shahsavari, E., Miranda, A.F. et al. Evaluating the efficacy of bioremediating a diesel-contaminated soil using ecotoxicological and bacterial community indices. Environ Sci Pollut Res 22, 14809–14819 (2015). https://doi.org/10.1007/s11356-015-4624-2

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  • DOI: https://doi.org/10.1007/s11356-015-4624-2

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