Abstract
The expansion in knowledge of the microbial community structure can play a vital role in the electrochemical features and operation of microbial fuel cells (MFCs). In this study, bacterial community composition in a dual chamber MFC fed with brewery waste was investigated for simultaneous electricity generation and azo dye degradation. A stable voltage was generated with a maximum power density of 305 and 269 mW m−2 for brewery waste alone (2000 mg L−1) and after the azo dye (200 mg L−1) addition, respectively. Azo dye degradation was confirmed by Fourier transform infrared spectroscopy (FT-IR) as peak corresponding to –N=N– (azo) bond disappeared in the dye metabolites. Microbial communities attached to the anode were analyzed by high-throughput 454 pyrosequencing of the 16S rRNA gene. Microbial community composition analysis revealed that Proteobacteria (67.3 %), Betaproteobacteria (30.8 %), and Desulfovibrio (18.3 %) were the most dominant communities at phylum, class, and genus level, respectively. Among the classified genera, Desulfovibrio most likely plays a major role in electron transfer to the anode since its outer membrane contains c-type cytochromes. At the genus level, 62.3 % of all sequences belonged to the unclassified category indicating a high level of diversity of microbial groups in MFCs fed with brewery waste and azo dye.
Highlights
• Azo dye degradation and stable bioelectricity generation was achieved in the MFC.
• Anodic biofilm was analyzed by high-throughput pyrosequencing of the 16S rRNA gene.
• Desulfovibrio (18.3 %) was the dominant genus in the classified genera.
• Of the genus, 62.3 % were unclassified, thereby indicating highly diverse microbes.
Similar content being viewed by others
References
Chen KC, Wu JY, Liou DJ, Hwang SCJ (2003) Decolorization of the textile dyes by newly isolated bacterial strains. J Biotechnol 101:57–68
Chengalroyen MD, Dabbs ER (2013) The microbial degradation of azo dyes: minireview. World J Microbiol Biotechnol 29:389–399
Chun J, Kim K, Lee J, Choi Y (2010) The analysis of oral microbial communities of wild-type and toll-like receptor 2-deficient mice using a 454 GS FLX Titanium pyrosequencer. BMC Microbiol 10:101
Cole JR, Wang Q, Cardenas E et al (2009) The Ribosomal Database Project: improved alignments and new tools for rRNA analysis. Nucleic Acids Res 37:D141–D145
Cord-ruwisch R (2008) Affinity of microbial fuel cell biofilm for the anodic potential. Environ Sci Technol 42:3828–3834
Dos Santos AB, Cervantes FJ, van Lier JB (2007) Review paper on current technologies for decolourisation of textile wastewaters: perspectives for anaerobic biotechnology. Bioresour Technol 98:2369–2385
Duteanu NM, Ghangrekar MM, Erable B, Scott K (2010) Microbial fuel cells—an option for wastewater treatment. Environ Eng Manag J 9:1069–1087
Fan Y, Sharbrough E, Liu H (2008) Quantification of the internal resistance distribution of microbial fuel cells. Environ Sci Technol 42:8101–8107
Jafary T, Rahimnejad M, Ghoreyshi AA et al (2013) Assessment of bioelectricity production in microbial fuel cells through series and parallel connections. Energy Convers Manag 75:256–262
Jia J, Tang Y, Liu B et al (2013) Electricity generation from food wastes and microbial community structure in microbial fuel cells. Bioresour Technol 144:94–99
Jung S, Regan JM (2007) Comparison of anode bacterial communities and performance in microbial fuel cells with different electron donors. Appl Microbiol Biotechnol 77:393–402
Kan J, Hsu L, Cheung A et al (2011) Current production by bacterial communities in microbial fuel cells enriched from wastewater sludge with different electron donors. Environ Sci Technol 45:1139–1146
Kang CS, Eaktasang N, Kwon D-Y, Kim HS (2014) Enhanced current production by Desulfovibrio desulfuricans biofilm in a mediator-less microbial fuel cell. Bioresour Technol 165:27–30
Kim JR, Jung SH, Regan JM, Logan BE (2007) Electricity generation and microbial community analysis of alcohol powered microbial fuel cells. Bioresour Technol 98:2568–2577
Lee HJ, Jung JY, Oh YK et al (2012) Comparative survey of rumen microbial communities and metabolites across one caprine and three bovine groups, using bar-coded pyrosequencing and 1H nuclear magnetic resonance spectroscopy. Appl Environ Microbiol 78:5983–5993
Logan BE (2010) Scaling up microbial fuel cells and other bioelectrochemical systems. Appl Microbiol Biotechnol 85:1665–1671
Logan BE, Hemelrs B, Rozendal R et al (2006) Microbial fuel cells: methodology and technology. Environ Sci Technol 40:5181–5192
Majumder D, Maity JP, Tseng M-J et al (2014) Electricity generation and wastewater treatment of oil refinery in microbial fuel cells using Pseudomonas putida. Int J Mol Sci 15:16772–16786
Ong S-A, Uchiyama K, Inadama D, Yamagiwa K (2009) Simultaneous removal of color, organic compounds and nutrients in azo dye-containing wastewater using up-flow constructed wetland. J Hazard Mater 165:696–703
Patil SA, Surakasi VP, Koul S et al (2009) Electricity generation using chocolate industry wastewater and its treatment in activated sludge based microbial fuel cell and analysis of developed microbial community in the anode chambe. Bioresour Technol 100:5132–5139
Qiao Y, Bao S, Li CM et al (2008) Nanostructured polyaniline/titanium dioxide composite anode for microbial fuel cells. ACS Nano 2:113–119
Qu Y, Cao X, Ma Q et al (2012) Aerobic decolorization and degradation of acid red B by a newly isolated Pichia sp TCL. J Hazard Mater 223–224:31–38
Rasool K, Woo SH, Lee DS (2013) Simultaneous removal of COD and Direct Red 80 in a mixed anaerobic sulfate-reducing bacteria culture. Chem Eng J 223:611–616
Reguera G, McCarthy KD, Mehta T et al (2005) Extracellular electron transfer via microbial nanowires. Nature 435:1098–1101
Sevda S, Dominguez-Benetton X, Vanbroekhoven K et al (2013) High strength wastewater treatment accompanied by power generation using air cathode microbial fuel cell. Appl Energy 105:194–206
Sharma Y, Parnas R, Li B (2011) Bioenergy production from glycerol in hydrogen producing bioreactors (HPBs) and microbial fuel cells (MFCs). Int J Hydrogen Energy 36:3853–3861
Solanki K, Subramanian S, Basu S (2013) Microbial fuel cells for azo dye treatment with electricity generation: a review. Bioresour Technol 131:564–571
Stolz A (2001) Basic and applied aspects in the microbial degradation of azo dyes. Appl Microbiol Biotechnol 56:69–80
Sun J, Hu Y-Y, Bi Z, Cao Y-Q (2009) Simultaneous decolorization of azo dye and bioelectricity generation using a microfiltration membrane air-cathode single-chamber microbial fuel cell. Bioresour Technol 100:3185–3192
Sun J, Hu Y, Hou B (2011) Electrochemical characteriztion of the bioanode during simultaneous azo dye decolorization and bioelectricity generation in an air-cathode single chambered microbial fuel cell. Electrochim Acta 56:6874–6879
Wang X, Feng YJ, Lee H (2008) Electricity production from beer brewery wastewater using single chamber microbial fuel cell. Water Sci Technol 57:1117–1121
Wang Z, Lee T, Lim B et al (2014) Microbial community structures differentiated in a single-chamber air-cathode microbial fuel cell fueled with rice straw hydrolysate. Biotechnol Biofuels 7:9
Watanabe K (2008) Recent developments in microbial fuel cell technologies for sustainable bioenergy. J Biosci Bioeng 106:528–536
Wen Q, Wu Y, Zhao L, Sun Q (2010) Production of electricity from the treatment of continuous brewery wastewater using a microbial fuel cell. Fuel 89:1381–1385
Yang S, Du F, Liu H (2012) Characterization of mixed-culture biofilms established in microbial fuel cells. Biomass Bioenergy 46:531–537
Yates MD, Kiely PD, Call DF et al (2012) Convergent development of anodic bacterial communities in microbial fuel cells. ISME J 6:2002–2013
Zhang Y, Min B, Huang L, Angelidaki I (2011) Electricity generation and microbial community response to substrate changes in microbial fuel cell. Bioresour Technol 102:1166–1173
Zhao Y, Li P, Wang X, Sun Y (2012) Influence of initial biofilm growth on electrochemical behavior in dual-chambered mediator microbial fuel cell. J Fuel Chem Technol 40:967–972
Zhi W, Ge Z, He Z, Zhang H (2014) Methods for understanding microbial community structures and functions in microbial fuel cells: a review. Bioresour Technol 171:461–468
Acknowledgments
This work was supported by the Human Resource Training Program for Regional Innovation and Creativity through the Ministry of Education and National Research Foundation (NRF) of Korea (NRF-2014H1C1A1066929). This study was also supported by grants (NRF-2013R1A1A4A01008000 and NRF-2009-0093819) through the ME and NRF of Korea. This research was also supported by the NRF grant by the Korea government (MSIP) (NRF-2015M2A7A1000194).
Author information
Authors and Affiliations
Corresponding author
Additional information
Responsible editor: Bingcai Pan
Electronic supplementary material
Below is the link to the electronic supplementary material.
ESM 1
(DOCX 197 kb)
Rights and permissions
About this article
Cite this article
Miran, W., Nawaz, M., Kadam, A. et al. Microbial community structure in a dual chamber microbial fuel cell fed with brewery waste for azo dye degradation and electricity generation. Environ Sci Pollut Res 22, 13477–13485 (2015). https://doi.org/10.1007/s11356-015-4582-8
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s11356-015-4582-8