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Isolation and molecular characterization of multidrug-resistant halophilic bacteria from shrimp farm effluents of Parangipettai coastal waters

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Abstract

Multidrug resistance of heterotrophic bacteria isolated from an aquaculture farm effluent in Parangipettai, at the southeastern coast of India, was investigated. In the initial screening, 27 antibiotic-resistant strains were isolated. All the strains were tested for antibiotic susceptibility against chloramphenicol with varying concentrations. From these, two highly resistant strains, i.e. S1 and S5, were isolated. The selected strains were identified by 16S ribosomal RNA (rRNA) sequencing techniques and confirmed as Bacillus pumilus and Bacillus flexus. Both the antibiotic-resistant strains were further utilized for multidrug susceptibility test by using various antibiotics. These two strains showed antibiotic resistance to 14 of 17 antibiotics tested. Both microdilution assay and well assay methods were used to determine the minimal inhibitory concentration (MIC) for the sensitive strains. Both the tests were shown to be almost similar. Our study highlights the occurrence of multidrug-resistant bacteria in the shrimp farm effluents.

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Acknowledgments

Authors wish to acknowledge Ministry of Earth Sciences (MoES), ICMAM-PD, Seawater Quality Monitoring Programme for the financial support (Grant No. ICMAM-PD/SWQM/CASMB/35/2012) and also we thank Authorities of Annamalai University for providing necessary facilities throughout the study duration.

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Authors and Affiliations

  1. CAS in Marine Biology, Faculty of Marine Sciences, Annamalai University, Parangipettai, 608 502, India

    Arumugam Sundaramanickam, Poominathan Suresh Kumar, Saravanan Kumaresan & Thangavel Balasubramanian

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  1. Arumugam Sundaramanickam
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  2. Poominathan Suresh Kumar
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  3. Saravanan Kumaresan
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  4. Thangavel Balasubramanian
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Correspondence to Arumugam Sundaramanickam.

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Sundaramanickam, A., Suresh Kumar, P., Kumaresan, S. et al. Isolation and molecular characterization of multidrug-resistant halophilic bacteria from shrimp farm effluents of Parangipettai coastal waters. Environ Sci Pollut Res 22, 11700–11707 (2015). https://doi.org/10.1007/s11356-015-4427-5

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  • DOI: https://doi.org/10.1007/s11356-015-4427-5

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