Abstract
In vivo and in vitro exposures were used to investigate the genotoxicity of mercuric chloride (HgCl2) to the marine snail, Planaxis sulcatus. The comet assay protocol was validated on gill cells exposed in vitro to hydrogen peroxide (H2O2, 0–50 μM). Snails were exposed in vivo for 96 h to HgCl2 (10, 20, 50, and 100 μg/l). Our results showed significant concentration-dependent increase in the tail DNA (TDNA) and olive tail moment (OTM) in exposed snails for all doses compared with controls. In vitro exposure to HgCl2 (10–100 μg/l) resulted in significantly higher values for TDNA at all concentrations. Our results showed that DNA damage increased in the gill cell with increasing exposure time. This study demonstrates the usefulness of comet assay for detection of DNA damage after exposure to HgCl2 and the sensitivity of marine snail P. sulcatus as a good candidate species for metal pollution.
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Acknowledgments
A part of the abstract was presented at the International Conference on Fisheries and Aquaculture (ICFA-2014) in Colombo, Sri Lanka on 09th and 10th September 2014. Authors would like to pay their deep sense of gratitude to the Director, CSIR-NIO for his whole-hearted cooperation and keen interest to carry out the work in this institute. They are indebted to Department of Biotechnology, New Delhi for providing fellowship to Mr. Jacky Bhagat. They also like to thank Analia Mesquita for her help in measurement of mercury. They also extend their thanks to the Council of Scientific and Industrial Research (CSIR) for providing the financial support and infrastructural facilities for Project No. PSC0206. This is contribution No 5714 of CSIR, NIO, Goa.
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We have reported concentration-dependent increase in tail DNA and olive tail moment measured by comet assay in marine gastropod Planaxis sulcatus exposed to in vivo and in vitro to HgCl2
Highlights
• An approach to evaluate genotoxicity of HgCl2 in marine gastropod was presented
• In vivo and in vitro effects of HgCl2in gill cells of gastropod was evaluated using comet assay
• Concentration dependent increase in tail DNA and olive tail moment is reported in exposed gastropods
• HgCl2 was found to be genotoxic to marine gastropod Planaxis sulcatus
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Bhagat, J., Ingole, B.S. Genotoxic potency of mercuric chloride in gill cells of marine gastropod Planaxis sulcatus using comet assay. Environ Sci Pollut Res 22, 10758–10768 (2015). https://doi.org/10.1007/s11356-015-4263-7
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DOI: https://doi.org/10.1007/s11356-015-4263-7