Advertisement

Environmental Science and Pollution Research

, Volume 22, Issue 11, pp 8182–8189 | Cite as

Assessment of DNA damage in floriculturists in southern Brazil

  • Camila Mörschbächer Wilhelm
  • Adriani Kunz Calsing
  • Luciano Basso da SilvaEmail author
Research Article

Abstract

The aim of this study was to assess possible genotoxic effects on floriculturists in a region of the state of Rio Grande do Sul, in the south of Brazil, using the micronucleus test (MN) and comet assay. Thirty-seven floriculturists and 37 individuals not exposed to pesticides participated in the study. The micronucleus test was performed with epithelial cells of the oral mucosa. In the microscopic analysis, 2000 cells were evaluated per subject, verifying the frequency of MN and the frequency of other nuclear abnormalities (nuclear buds, binucleated cells, and karyorrhexis). For the comet assay in the peripheral blood lymphocytes, 100 cells were classified in five classes, according to the migration of DNA fragments, thereby generating the frequency of damaged cells and the damage index. There was no difference between the exposed and control groups in the frequencies of MN and other nuclear abnormalities in the epithelial cells of the oral mucosa. However, the comet assay showed that both the frequency of DNA damaged cells and the damage index were significantly greater in the exposed group. The results therefore indicate that floriculturists are exposed to mixtures of pesticides with genotoxic potential.

Keywords

Occupational exposure Floriculturists Pesticides Micronucleus test Comet assay 

References

  1. Anderson D, Yu TW, Phillips BJ, Schmezer P (1994) The effect of various antioxidants and other modifying agents on oxygen-radical-generated DNA damage in human lymphocytes in the COMET assay. Mutat Res 307:261–271CrossRefGoogle Scholar
  2. Benedetti D, Nunes E, Sarmento M, Porto C, Dos Santos CE, Dias JF, Da Silva J (2013) Genetic damage in soybean workers exposed to pesticides: evaluation with the comet and buccal micronucleus cytome assays. Mutat Res 752:28–33. doi: 10.1016/j.mrgentox.2013.01.001 CrossRefGoogle Scholar
  3. Bolognesi C (2003) Genotoxicity of pesticides: a review of human biomonitoring studies. Mutat Res 543:251–272CrossRefGoogle Scholar
  4. Bolognesi C, Merlo F, Rabboni R, Roggieri P, Reggiardo G, Abbondandolo A (1995) Genotoxic risk from occupational exposure to pesticides in floriculture. Clin Chem 41:1919–1922Google Scholar
  5. Bolognesi C, Perrone E, Landini E (2002) Micronucleus monitoring of a floriculturist population from western Liguria, Italy. Mutagenesis 17:391–397Google Scholar
  6. Bolognesi C, Landini E, Perrone E, Roggieri P (2004) Cytogenetic biomonitoring of a floriculturist population in Italy: micronucleus analysis by fluorescence in situ hybridization (FISH) with an all-chromosome centromeric probe. Mutat Res 557:109–117CrossRefGoogle Scholar
  7. Bolognesi C, Knasmueller S, Nersesyan A, Thomas P, Fenech M (2013) The HUMNxl scoring criteria for different cell types and nuclear anomalies in the buccal micronucleus cytome assay—an update and expanded photogallery. Mutat Res 753:100–113. doi: 10.1016/j.mrrev.2013.07.002 CrossRefGoogle Scholar
  8. Bull S, Fletcher K, Boobis AR, Battershill JM (2006) Evidence for genotoxicity of pesticides in pesticide applicators: a review. Mutagenesis 21:93–103CrossRefGoogle Scholar
  9. Castillo-Cadena J, Tenorio-Vieyra LE, Quintana-Carabia AI, García-Fabila MM, Juan ER, Madrigal-Bujaidar E (2006) Determination of DNA damage in floriculturists exposed to mixtures of pesticides. J Biomed Biotechnol 2006:97896CrossRefGoogle Scholar
  10. Da Silva FR, Da Silva J, Allgayer MC, Simon CF, Dias JF, Dos Santos CE, Salvador M, Branco C, Schneider NB, Kahl V, Rohr P, Kvitko K (2012) Genotoxic biomonitoring of tobacco farmers: biomarkers of exposure, of early biological effects and of susceptibility. J Hazard Mater 225–226:81–90. doi: 10.1016/j.jhazmat.2012.04.074 CrossRefGoogle Scholar
  11. De Ferrari M, Artuso M, Bonassi S, Bonatti S, Cavalieri Z, Pescatore D, Marchini E, Pisano V, Abbondandolo A (1991) Cytogenetic biomonitoring of an Italian population exposed to pesticides: chromosome aberration and sister-chromatid exchange analysis in peripheral blood lymphocytes. Mutat Res 260:105–113CrossRefGoogle Scholar
  12. Dulout FN, Pastori MC, Olivero OA, Cid MG, Loria D, Matos E, Sobel N, Bujan EC, Albiano N (1985) Sister-chromatid exchanges and chromosomal aberrations in a population exposed to pesticides. Mutat Res 143:237–244CrossRefGoogle Scholar
  13. Ellinger-Ziegelbauer H, Aubrecht J, Kleinjans JC, Ahr HJ (2009) Application of toxicogenomics to study mechanisms of genotoxicity and carcinogenicity. Toxicol Lett 186:36–44. doi: 10.1016/j.toxlet.2008.08.017 CrossRefGoogle Scholar
  14. Ergene S, Çelik A, Çavaş T, Kaya F (2007) Genotoxic biomonitoring study of population residing in pesticide contaminated regions in Göksu Delta: micronucleus, chromosomal aberrations and sister chromatid exchanges. Environ Int 33:877–885CrossRefGoogle Scholar
  15. Falck GC, Hirvonen A, Scarpato R, Saarikoski ST, Migliore L, Norppa H (1999) Micronuclei in blood lymphocytes and genetic polymorphism for GSTM1, GSTT1 and NAT2 in pesticide-exposed greenhouse workers. Mutat Res 441:225–237CrossRefGoogle Scholar
  16. Fenech M, Holland N, Chang WP, Zeiger E, Bonassi S (1999) The HUman MicroNucleus Project—an international collaborative study on the use of the micronucleus technique for measuring DNA damage in humans. Mutat Res 428:271–283CrossRefGoogle Scholar
  17. Gómez-Arroyo S, Díaz-Sánchez Y, Meneses-Pérez MA, Villalobos-Pietrini R, León-Rodríguez JD (2000) Cytogenetic biomonitoring in a Mexican floriculture worker group exposed to pesticides. Mutat Res 466:117–124CrossRefGoogle Scholar
  18. Holland N, Bolognesi C, Kirsch-Volders M, Bonassi S, Zeiger E, Knasmueller S, Fenech M (2008) The micronucleus assay in human buccal cells as a tool for biomonitoring DNA damage: the HUMN project perspective on current status and knowledge gaps. Mutat Res 659:93–108. doi: 10.1016/j.mrrev.2008.03.007 CrossRefGoogle Scholar
  19. Lander F, Knudsen LE, Gamborg MO, Järventaus H, Norppa H (2000) Chromosome aberrations in pesticide-exposed greenhouse. Scand J Work Environ Health 26:436–442CrossRefGoogle Scholar
  20. Lucero L, Pastor S, Suárez S, Durbán R, Gómez C, Parrón T, Creus A, Marcos R (2000) Cytogenetic biomonitoring of Spanish greenhouse workers exposed to pesticides: micronuclei analysis in peripheral blood lymphocytes and buccal epithelial cells. Mutat Res 464:255–262CrossRefGoogle Scholar
  21. Mostafalou S, Abdollahi M (2013) Pesticides and human chronic diseases: evidences, mechanisms, and perspectives. Toxicol Appl Pharmacol 268:157–177. doi: 10.1016/j.taap.2013.01.025 CrossRefGoogle Scholar
  22. Nadin SB, Vargas-Roig LM, Ciocca DR (2001) A silver staining method for single-cell gel assay. J Histochem Cytochem 49:1183–1186CrossRefGoogle Scholar
  23. Piperakis SM, Petrakou E, Tsilimigaki S, Sagnou M, Monogiudis E, Haniotakis G, Karkaseli H, Sarikaki E (2003) Biomonitoring with the comet assay of Greek greenhouse workers exposed to pesticides. Environ Mol Mutagen 41:104–110CrossRefGoogle Scholar
  24. Pitarque M, Creus A, Marcos R, Hughes JA, Anderson D (1999) Examination of various biomarkers measuring genotoxic endpoints from Barcelona airport personnel. Mutat Res 440:195–204CrossRefGoogle Scholar
  25. Remor AP, Totti CC, Moreira DA, Dutra GP, Heuser VD, Boeira JM (2009) Occupational exposure of farm workers to pesticides: biochemical parameters and evaluation of genotoxicity. Environ Int 35:273–278. doi: 10.1016/j.envint.2008.06.011 CrossRefGoogle Scholar
  26. Ribeiro MG, Colasso CG, Monteiro PP, Pedreira Filho WR, Yonamine M (2012) Occupational safety and health practices among flower greenhouses workers from Alto Tietê region (Brazil). Sci Total Environ 416:121–126. doi: 10.1016/j.scitotenv.2011.11.002 CrossRefGoogle Scholar
  27. Sailaja N, Chandrasekhar M, Rekhadevi PV, Mahboob M, Rahman MF, Vuyyuri SB, Danadevi K, Hussain SA, Grover P (2006) Genotoxic evaluation of workers employed in pesticide production. Mutat Res 609:74–80CrossRefGoogle Scholar
  28. Scarpato R, Migliore L, Angotzi G, Fedi A, Miligi L, Loprieno N (1996) Cytogenetic monitoring of a group of Italian floriculturists: no evidence of DNA damage related to pesticide exposure. Mutat Res 367:73–82CrossRefGoogle Scholar
  29. Shaham J, Kaufman Z, Gurvich R, Levi Z (2001) Frequency of sister-chromatid exchange among greenhouse farmers exposed to pesticides. Mutat Res 491:71–80CrossRefGoogle Scholar
  30. Singh S, Kumar V, Thakur S, Banerjee BD, Chandna S, Rautelaa RS, Grover SS, Rawat DS, Pasha ST, Jain SK, Ichhpujani RL, Rai A (2011) DNA damage and cholinesterase activity in occupational workers exposed to pesticides. Environ Toxicol Pharmacol 31:278–285. doi: 10.1016/j.etap.2010.11.005 CrossRefGoogle Scholar
  31. Tice RR, Agurell E, Anderson D, Burlinson B, Hartmann A, Kobayashi H, Miyamae Y, Rojas E, Ryu JC, Sasaki YF (2000) Single cell gel/comet assay: guidelines for in vitro and in vivo genetic toxicology testing. Environ Mol Mutagen 35:206–221CrossRefGoogle Scholar
  32. Tolbert PE, Shy CM, Allen JW (1992) Micronuclei and other nuclear anomalies in buccal smears: methods development. Mutat Res 271:69–77CrossRefGoogle Scholar
  33. Valverde M, Rojas E (2009) Environmental and occupational biomonitoring using the Comet assay. Mutat Res 681:93–109. doi: 10.1016/j.mrrev.2008.11.001 CrossRefGoogle Scholar
  34. World Health Organization (2010) The WHO recommended classification of pesticides by hazard and guidelines to classification: 2009. http://www.inchem.org/documents/pds/pdsother/class_2009.pdf>. Accessed April 29 2013.

Copyright information

© Springer-Verlag Berlin Heidelberg 2014

Authors and Affiliations

  • Camila Mörschbächer Wilhelm
    • 1
  • Adriani Kunz Calsing
    • 2
  • Luciano Basso da Silva
    • 3
    Email author
  1. 1.Curso de BiomedicinaUniversidade FeevaleNovo HamburgoBrazil
  2. 2.Curso de FarmáciaUniversidade FeevaleNovo HamburgoBrazil
  3. 3.Programa de Pós-Graduação em Qualidade Ambiental, Grupo de Pesquisa em Saúde Humana e AmbienteUniversidade FeevaleNovo HamburgoBrazil

Personalised recommendations