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Protective effect of Mn(III)–desferrioxamine B upon oxidative stress caused by ozone and acid rain in the Brazilian soybean cultivar Glycine max “Sambaiba”

Environmental Science and Pollution Research volume 22, pages 5315–5324 (2015)Cite this article

Abstract

This study aimed to investigate the effects of the Mn complex (Mn(III)–desferrioxamine B (MnDFB)) on oxidative stress in the Brazilian soybean cultivar Glycine max “Sambaiba” following exposure to ozone and acid rain. We determined the suitable dose of MnDFB to apply to G. max seedlings using a dose–response curve. The highest superoxide dismutase (SOD) activity and Mn content in leaves were found upon the application of 8 μM MnDFB. Thus, G. max seedlings pretreated with 8 μM MnDFB were individually exposed to ozone and acid rain simulated. Pretreatment with MnDFB reduced lipid peroxidation upon ozone exposure and increased SOD activity in leaves; it did not alter the metal content in any part of the plant. Conversely, following acid rain exposure, neither the metal content in leaves nor SOD enzyme activity were directly affected by MnDFB, unlike pH. Our findings demonstrated that exogenous MnDFB application before ozone exposure may modulate the MnSOD, Cu/ZnSOD, and FeSOD activities to combat the ROS excess in the cell. Here, we demonstrated that the applied dose of MnDFB enhances antioxidative defenses in soybean following exposure to acid rain and especially to ozone.

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Acknowledgments

The authors acknowledge financial support from CAPES/PNADB, FAPESP (02/04751-6; 12/11663-8) and CNPq (Brazilian funding agencies) and Embrapa Soja for donating soybean seed. We express our gratitude to Dr. Salete Aparecida Gaziola for her assistance with analytical work and Mr. Thiago Andrade for technical assistance with the synthesis of MnDFB. S.R Souza and R.A. Azevedo are grateful to CNPq for research fellowships.

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Authors and Affiliations

  1. Instituto de Botânica, Núcleo de Ecologia, 01061-970, São Paulo, Brazil

    Jéssica Bordotti Nobre Esposito & Silvia Ribeiro de Souza

  2. Instituto de Química, Universidade de São Paulo, 05508-000, São Paulo, Brazil

    Breno Pannia Esposito

  3. Escola Superior de Agronomia Luiz de Queiroz, Universidade de São Paulo, 13400-970, Piracicaba, Brazil

    Ricardo Antunes Azevedo

  4. Universidade Federal do ABC, UFABC, São Paulo, 0920-10971 São Paulo, Brazil

    Luciano Soares Cruz

  5. Universidade Federal de Viçosa, Minas Gerais, 36570-900 Viçosa, Brazil

    Luzimar Campos da Silva

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  1. Jéssica Bordotti Nobre Esposito
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  2. Breno Pannia Esposito
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  3. Ricardo Antunes Azevedo
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  6. Silvia Ribeiro de Souza
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Correspondence to Silvia Ribeiro de Souza.

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Esposito, J.B.N., Esposito, B.P., Azevedo, R.A. et al. Protective effect of Mn(III)–desferrioxamine B upon oxidative stress caused by ozone and acid rain in the Brazilian soybean cultivar Glycine max “Sambaiba”. Environ Sci Pollut Res 22, 5315–5324 (2015). https://doi.org/10.1007/s11356-014-3951-z

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Keywords

  • Soybean
  • Abiotic stress
  • Air pollution
  • Antioxidant enzymes
  • Manganese complex
  • Exogenous chemicals