Environmental Science and Pollution Research

, Volume 22, Issue 9, pp 6407–6413 | Cite as

A sensitive analytical procedure for monitoring acrylamide in environmental water samples by offline SPE–UPLC/MS/MS

  • Anne TogolaEmail author
  • Charlotte Coureau
  • Anne-Gwenaëlle Guezennec
  • Solène Touzé
Degradation and transfer of polyacrylamide based floculents in sludges and industrial and natural waters


The presence of acrylamide in natural systems is of concern from both environmental and health points of view. We developed an accurate and robust analytical procedure (offline solid phase extraction combined with UPLC/MS/MS) with a limit of quantification (20 ng L−1) compatible with toxicity threshold values. The optimized (considering the nature of extraction phases, sampling volumes, and solvent of elution) solid phase extraction (SPE) was validated according to ISO Standard ISO/IEC 17025 on groundwater, surface water, and industrial process water samples. Acrylamide is highly polar, which induces a high variability during the SPE step, therefore requiring the use of C13-labeled acrylamide as an internal standard to guarantee the accuracy and robustness of the method (uncertainty about 25 % (k = 2) at limit of quantification level). The specificity of the method and the stability of acrylamide were studied for these environmental media, and it was shown that the method is suitable for measuring acrylamide in environmental studies.


Acrylamide Analysis SPE LC/MS/MS Groundwater Process water Surface water 



This work was funded by the French National Research Agency (ANR) through the AQUAPOL project (ANR-2010-CESA-019-03).


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Copyright information

© Springer-Verlag Berlin Heidelberg 2014

Authors and Affiliations

  • Anne Togola
    • 1
    Email author
  • Charlotte Coureau
    • 1
  • Anne-Gwenaëlle Guezennec
    • 1
  • Solène Touzé
    • 1
  1. 1.Bureau de recherches géologiques et minières (BRGM)OrléansFrance

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