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Oxidative stress and brain mitochondria swelling induced by endosulfan and protective role of quercetin in rat

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Abstract

The neurological damages resulted by endosulfan poisoning is not completely elucidated, especially in cellular organelles such as mitochondria. In the present study, the pro-oxidant effect of endosulfan on brain mitochondria was first investigated. Gavages of endosulfan into rats at the dose of 2 mg/kg induced oxidative stress in this organelle since it provokes a significant reduction of catalase (CAT), superoxide dismutase (SOD), and glutathione (GSH) level. In addition, a significant increase in mitochondria swelling and malondialdehyde (MDA) levels were observed in neuronal mitochondria, indicating clearly an intense peroxidation within mitochondria. Second, the protective effect of quercetin (QE) (10 mg/kg) against endosulfan-induced oxidative stress in mitochondria was also assessed. Indeed, the pretreatment of rats with QE protects brain mitochondria from oxidative stress, lipid peroxidation, and mitochondria swelling induced by endosulfan. The activities of antioxidant enzymes and the mitochondrial content of GSH and MDA were returned to control values. Thus, although endosulfan can have neurotoxic effects in brain rats, this toxicity can be prevented by quercetin.

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Authors and Affiliations

  1. Laboratory of Molecular and Cell Biology (BMC), University of Jijel, Jijel, Algeria

    Zhoura Lakroun, Mohamed Kebieche & Asma Lahouel

  2. Department of Animal Biology, University of Mentouri 1, Constantine, Algeria

    Zhoura Lakroun & Djamila Zama

  3. Laboratory of Food Neurotoxicology and Bioactivity, University of Metz, Metz, France

    Mohamed Kebieche, Frederique Desor & Rachid Soulimani

Authors
  1. Zhoura Lakroun
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  2. Mohamed Kebieche
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  3. Asma Lahouel
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  4. Djamila Zama
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  5. Frederique Desor
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  6. Rachid Soulimani
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Correspondence to Mohamed Kebieche.

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Responsible editor: Philippe Garrigues

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Lakroun, Z., Kebieche, M., Lahouel, A. et al. Oxidative stress and brain mitochondria swelling induced by endosulfan and protective role of quercetin in rat. Environ Sci Pollut Res 22, 7776–7781 (2015). https://doi.org/10.1007/s11356-014-3885-5

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  • DOI: https://doi.org/10.1007/s11356-014-3885-5

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