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Relationship between viral detection and turbidity in a watershed contaminated with group A rotavirus

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Abstract

Enteric viruses are present in aquatic environments due to contamination by raw sewage, even in the absence of fecal coliforms, which are considered to be significant indicators when it comes to microbial water quality assessment. This study investigated the presence of group A rotavirus (RVA) in surface water from a river basin in Minas Gerais, Brazil, assessing the influence of the urbanization, the rainfall, and the microbiological and physico-chemical parameters regarding water quality. Forty-eight surface water samples collected in urbanized and non-urbanized areas, both in dry and rainy periods, were obtained throughout the study. The viral particles were concentrated by adsorption-elution in a negatively charged membrane. Fecal coliforms, as well as physico-chemical water parameters were determined at each point in all collections. The RVA was detected in 62.5 % (30/48) of the water samples through a real-time PCR assay. All the sequenced RVA strains belonged to genotype I1. The RVA was detected in 50.0 % (11/22) of the water samples regarded as being acceptable by current microbiological standards. The presence of the RVA and the viral load were influenced by the collection area (p < 0.05). It was also observed a significant association between the RVA and detecting the turbidity of water (p < 0.05). The collected data showed a high level of contamination in this watershed by the discharge of raw sewage, highlighting the need for urgent measures to improve water quality, ensuring the safe use of this matrix.

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Acknowledgments

We thank the Sanitation Company, Cesama, which authorized our access to the dam reservoir of São Pedro. We also thank the Military Police Company of Environmental and the Military Firefighters of Juiz de Fora, state of Minas Gerais, which provided the boat and the staff for surface water collects in the dam reservoir, thus allowing the safe development of this study.

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None declared.

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Authors and Affiliations

  1. Laboratory of Virology, Microbiology Department, Institute of Biological Science, Federal University of Juiz de Fora, Juiz de Fora, Minas Gerais, Brazil

    Andrêssa Silvino Ferreira Assis, Lucas Taffarel Cruz, Aline Siqueira Ferreira & Maria Luzia da Rosa e Silva

  2. Laboratory for Analysis of Food and Water, Faculty of Pharmacy, Federal University of Juiz de Fora, Juiz de Fora, Minas Gerais, Brazil

    Martha Eunice Bessa & Miriam Aparecida de Oliveira Pinto

  3. Laboratory of Comparative and Environmental Virology, Oswaldo Cruz Institute, Rio de Janeiro, RJ, Brazil

    Carmen Baur Vieira & Marize Pereira Miagostovich

  4. Brazilian Agricultural Research Corporation, Embrapa Dairy Cattle, Rua Eugênio do Nascimento, 610 - Dom Bosco, Juiz de Fora, Minas Gerais, 36038-330, Brazil

    Marcelo Henrique Otenio

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  1. Andrêssa Silvino Ferreira Assis
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  2. Lucas Taffarel Cruz
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  3. Aline Siqueira Ferreira
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  4. Martha Eunice Bessa
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  5. Miriam Aparecida de Oliveira Pinto
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  6. Carmen Baur Vieira
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  7. Marcelo Henrique Otenio
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  8. Marize Pereira Miagostovich
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  9. Maria Luzia da Rosa e Silva
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Correspondence to Marcelo Henrique Otenio.

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Assis, A.S.F., Cruz, L.T., Ferreira, A.S. et al. Relationship between viral detection and turbidity in a watershed contaminated with group A rotavirus. Environ Sci Pollut Res 22, 6886–6897 (2015). https://doi.org/10.1007/s11356-014-3874-8

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