Environmental Science and Pollution Research

, Volume 22, Issue 9, pp 6414–6422 | Cite as

Direct injection method for HPLC/MS/MS analysis of acrylamide in aqueous solutions: application to adsorption experiments

  • Ines MnifEmail author
  • Charlotte Hurel
  • Nicolas Marmier
Degradation and transfer of polyacrylamide based floculents in sludges and industrial and natural waters


Polyacrylamides are polymers used in many fields and represent the main source of release of the highly toxic acrylamide in the environment. In this work, a simple, rapid, and sensitive analytical method was developed with HPLC/MS/MS and direct injection for acrylamide analysis in water and adsorption samples. AFNOR standards NF T90-210 and NF T90-220 were used for the analytical method validation and uncertainty estimation. Limit of quantification (LOQ) for acrylamide was 1 μg/L, and accuracy was checked at three acrylamide levels (1, 6, and 10 μg/L). Uncertainties were estimated at 34.2, 22, and 12.4 % for acrylamide concentrations at LOQ, 6 μg/L, and 10 μg/L, respectively. Acrylamide adsorption on clays (kaolinite, illite) and sludge was then studied as a function of pH, time, and acrylamide concentrations. Acrylamide adsorption on kaolinite, illite, and sludge was found to be very weak since adsorption percentages were inferior to 10 %, whatever the pH value and the initial acrylamide concentration. The low affinity of acrylamide for clays and sludge is likely due to its hydrophilic property, small size, and charge neutrality.


Acrylamide HPLC/MS/MS Quantification Adsorption Clays Sludge 



The authors thank the ANR CES for the financial support of the Aquapol project and Anne Togola from BRGM for fruitful discussions about analytical optimizations.

Supplementary material

11356_2014_3774_MOESM1_ESM.docx (44 kb)
ESM 1 (DOCX 44 kb)


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Copyright information

© Springer-Verlag Berlin Heidelberg 2014

Authors and Affiliations

  1. 1.ECOMERSUniversity Nice Sophia AntipolisNice Cedex 2France

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