Advertisement

Environmental Science and Pollution Research

, Volume 22, Issue 2, pp 1127–1132 | Cite as

In vivo toxicity of orally administrated silicon dioxide nanoparticles in healthy adult mice

  • Ramin Hassankhani
  • Mohammad Esmaeillou
  • Ali Asghar Tehrani
  • Keyvan Nasirzadeh
  • Fatemeh Khadir
  • Hamid MaadiEmail author
Research Article

Abstract

The increasing use of silica nanoparticles (SiNPs) in various applications including industrial, agriculture, and medicine has raised concerns about their potential risks to human health. Various nanotoxicity researches have been done on the assessment of SiNPs’ toxic effects; however, a few in vivo investigations exist. In this investigation, an in vivo study was done in order to evaluate the oral toxicity of SiNPs. The biochemical levels of 19 different serum parameters were assessed. Moreover, the histopathological changes have been examined as well. We showed that SiNPs with diameters of 10–15 nm in size can cause significant changes in albumin, cholesterol, triglyceride, total protein, urea, HDL, and LDL as well as in alkaline phosphatase and aspartate aminotransferase activity. In addition, histopathological examinations demonstrated that SiNPs have toxic effects on various tissues including liver, kidney, lung, and testis.

Keywords

SiO2 nanoparticles Oral gavage Liver Kidney Testis Lung Biochemical elements 

References

  1. Ahmad J, Ahamed M, Akhtar MJ, Alrokayan SA, Siddiqui MA, Musarrat J, Al-Khedhairy AA (2012) Apoptosis induction by silica nanoparticles mediated through reactive oxygen species in human liver cell line HepG2. Toxicol Appl Pharmacol 259:160–168CrossRefGoogle Scholar
  2. Chen Z, Meng H, Xing G, Chen C, Zhao Y, Jia G, Wang T, Yuan H, Ye C, Zhao F et al (2006) Acute toxicological effects of copper nanoparticles in vivo. Toxicol Lett 163:109–120CrossRefGoogle Scholar
  3. Cho M, Cho WS, Choi M, Kim SJ, Han BS, Kim SH, Kim HO, Sheen YY, Jeong J (2009) The impact of size on tissue distribution and elimination by single intravenous injection of silica nanoparticles. Toxicol Lett 189:177–183CrossRefGoogle Scholar
  4. Esmaeillou M, Moharamnejad M, Hsankhani R, Tehrani AA, Maadi H (2013) Toxicity of ZnO nanoparticles in healthy adult mice. Environ Toxicol Pharmacol 35:67–71CrossRefGoogle Scholar
  5. Garcia TX, Costa GM, Franca LR, Hofmann MC (2014) Sub-acute intravenous administration of silver nanoparticles in male mice alters Leydig cell function and testosterone levels. Reprod Toxicol (Elmsford, NY) 45C:59–70CrossRefGoogle Scholar
  6. Gowda S, Desai PB, Kulkarni SS, Hull VV, Math AAK, Vernekar SN (2010) Markers of renal function tests. N Am J Med Sci 2:170Google Scholar
  7. Green RM, Flamm S (2002) AGA technical review on the evaluation of liver chemistry tests. Gastroenterology 123:1367–1384CrossRefGoogle Scholar
  8. Gromadzka-Ostrowska J, Dziendzikowska K, Lankoff A, Dobrzynska M, Instanes C, Brunborg G, Gajowik A, Radzikowska J, Wojewodzka M, Kruszewski M (2012) Silver nanoparticles effects on epididymal sperm in rats. Toxicol Lett 214:251–258CrossRefGoogle Scholar
  9. Guo LL, Liu XH, Qin DX, Gao L, Zhang HM, Liu JY, Cui YG (2009) Effects of nanosized titanium dioxide on the reproductive system of male mice. Zhonghua Nan Ke Xue Natl J Androl 15:517–522Google Scholar
  10. Guo M, Xu X, Yan X, Wang S, Gao S, Zhu S (2013) In vivo biodistribution and synergistic toxicity of silica nanoparticles and cadmium chloride in mice. J Hazard Mater 260:780–788CrossRefGoogle Scholar
  11. Hansen SF, Michelson ES, Kamper A, Borling P, Stuer-Lauridsen F, Baun A (2008) Categorization framework to aid exposure assessment of nanomaterials in consumer products. Ecotoxicology (London, England) 17:438–447CrossRefGoogle Scholar
  12. He Q, Zhang Z, Gao F, Li Y, Shi J (2011) In vivo biodistribution and urinary excretion of mesoporous silica nanoparticles: effects of particle size and PEGylation. Small (Weinheim an der Bergstrasse, Germany) 7:271–280CrossRefGoogle Scholar
  13. Huang X, Li L, Liu T, Hao N, Liu H, Chen D, Tang F (2011) The shape effect of mesoporous silica nanoparticles on biodistribution, clearance, and biocompatibility in vivo. ACS Nano 5:5390–5399CrossRefGoogle Scholar
  14. Jia F, Sun Z, Yan X, Zhou B, Wang J (2014) Effect of pubertal nano-TiO2 exposure on testosterone synthesis and spermatogenesis in mice. Arch Toxicol 88:781–788Google Scholar
  15. Kim M-H, Na H-K, Kim Y-K, Ryoo S-R, Cho HS, Lee KE, Jeon H, Ryoo R, Min D-H (2011) Facile synthesis of monodispersed mesoporous silica nanoparticles with ultralarge pores and their application in gene delivery. ACS Nano 5:3568–3576CrossRefGoogle Scholar
  16. Kumar R, Roy I, Ohulchanskky TY, Vathy LA, Bergey EJ, Sajjad M, Prasad PN (2010) In vivo biodistribution and clearance studies using multimodal organically modified silica nanoparticles. ACS Nano 4:699–708CrossRefGoogle Scholar
  17. Lin W, Huang YW, Zhou XD, Ma Y (2006) In vitro toxicity of silica nanoparticles in human lung cancer cells. Toxicol Appl Pharmacol 217:252–259CrossRefGoogle Scholar
  18. Liu T, Li L, Fu C, Liu H, Chen D, Tang F (2012) Pathological mechanisms of liver injury caused by continuous intraperitoneal injection of silica nanoparticles. Biomaterials 33:2399–2407CrossRefGoogle Scholar
  19. Minuk GY (1998) Canadian Association of Gastroenterology Practice Guidelines: evaluation of abnormal liver enzyme tests. Can J Gastroenterol J Can Gastroenterol 12:417–421Google Scholar
  20. Morishita Y, Yoshioka Y, Satoh H, Nojiri N, Nagano K, Abe Y, Kamada H, Tsunoda S-I, Nabeshi H, Yoshikawa T et al (2012) Distribution and histologic effects of intravenously administered amorphous nanosilica particles in the testes of mice. Biochem Biophys Res Commun 420:297–301CrossRefGoogle Scholar
  21. Nishimori H, Kondoh M, Isoda K, Tsunoda S, Tsutsumi Y, Yagi K (2009a) Histological analysis of 70-nm silica particles-induced chronic toxicity in mice. Eur J Pharm Biopharm Off J Arbeitsgemeinschaft PharmVerfahrenstechnik eV 72:626–629CrossRefGoogle Scholar
  22. Nishimori H, Kondoh M, Isoda K, Tsunoda S, Tsutsumi Y, Yagi K (2009b) Silica nanoparticles as hepatotoxicants. Eur J Pharm Biopharm Off J Arbeitsgemeinschaft PharmVerfahrenstechnik eV 72:496–501CrossRefGoogle Scholar
  23. Passagne I, Morille M, Rousset M, Pujalte I, L’Azou B (2012) Implication of oxidative stress in size-dependent toxicity of silica nanoparticles in kidney cells. Toxicology 299:112–124CrossRefGoogle Scholar
  24. Rahman M, Laurent S, Tawil N, Yahia LH, Mahmoudi M (2013) Nanoparticle and protein corona. In Protein-nanoparticle interactions (Springer Berlin Heidelberg), pp. 21–44Google Scholar
  25. Ranjbakhsh E, Bordbar AK, Abbasi M, Khosropour AR, Shams E (2012) Enhancement of stability and catalytic activity of immobilized lipase on silica-coated modified magnetite nanoparticles. Chem Eng J 179:272–276CrossRefGoogle Scholar
  26. Rees D, Murray J (2007) Silica, silicosis and tuberculosis. Int J Tuberc Lung Dis Off J Int Union Against Tuberc Lung Dis 11:474–484Google Scholar
  27. Sun L, Li Y, Liu X, Jin M, Zhang L, Du Z, Guo C, Huang P, Sun Z (2011) Cytotoxicity and mitochondrial damage caused by silica nanoparticles. Toxicol In Vitro Int J Publ Assoc BIBRA 25:1619–1629CrossRefGoogle Scholar
  28. van der Zande M, Vandebriel R, Groot M, Kramer E, Herrera Rivera Z, Rasmussen K, Ossenkoppele J, Tromp P, Gremmer E, Peters R et al (2014) Sub-chronic toxicity study in rats orally exposed to nanostructured silica. Part Fibre Toxicol 11:1–19Google Scholar
  29. Wang B, Feng W, Wang M, Wang T, Gu Y, Zhu M, Ouyang H, Shi J, Zhang F, Zhao Y et al (2008) Acute toxicological impact of nano- and submicro-scaled zinc oxide powder on healthy adult mice. J Nanopart Res 10:263–276CrossRefGoogle Scholar
  30. Xie G, Sun J, Zhong G, Shi L, Zhang D (2010) Biodistribution and toxicity of intravenously administered silica nanoparticles in mice. Arch Toxicol 84:183–190CrossRefGoogle Scholar
  31. Yamashita K, Yoshioka Y, Higashisaka K, Mimura K, Morishita Y, Nozaki M, Yoshida T, Ogura T, Nabeshi H, Nagano K et al (2011) Silica and titanium dioxide nanoparticles cause pregnancy complications in mice. Nat Nanotechnol 6:321–328CrossRefGoogle Scholar
  32. Yang X, Liu J, He H, Zhou L, Gong C, Wang X, Yang L, Yuan J, Huang H, He L et al (2010) SiO2 nanoparticles induce cytotoxicity and protein expression alteration in HaCaT cells. Part Fibre Toxicol 7:1CrossRefGoogle Scholar
  33. Ye Y, Liu J, Chen M, Sun L, Lan M (2010a) In vitro toxicity of silica nanoparticles in myocardial cells. Environ Toxicol Pharmacol 29:131–137CrossRefGoogle Scholar
  34. Ye Y, Liu J, Xu J, Sun L, Chen M, Lan M (2010b) Nano-SiO2 induces apoptosis via activation of p53 and Bax mediated by oxidative stress in human hepatic cell line. Toxicol In Vitro Int J Publ Assoc BIBRA 24:751–758CrossRefGoogle Scholar
  35. Yuan L, Tang Q, Yang D, Zhang JZ, Zhang F, Hu J (2011) Preparation of pH-responsive mesoporous silica nanoparticles and their application in controlled drug delivery. J Phys Chem C 115:9926–9932CrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 2014

Authors and Affiliations

  • Ramin Hassankhani
    • 1
  • Mohammad Esmaeillou
    • 2
  • Ali Asghar Tehrani
    • 3
  • Keyvan Nasirzadeh
    • 4
  • Fatemeh Khadir
    • 3
  • Hamid Maadi
    • 2
    Email author
  1. 1.Department of Science, Urmia BranchIslamic Azad UniversityUrmiaIran
  2. 2.Young Researchers and Elite Club, Urmia BranchIslamic Azad UniversityUrmiaIran
  3. 3.Department of Pathology, Faculty of Veterinary MedicineUrmia UniversityUrmiaIran
  4. 4.Industrial and Environmental Biotechnology DivisionNational Institute of Genetic Engineering and Biotechnology (NIGEB)TehranIran

Personalised recommendations