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Relationship between variation of seasonal temperature and extent of occupational exposure to phthalates

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Spot urine samples were collected in summer and winter season to examine the association between temperature variation and phthalate concentration in an occupationally exposed group. We analysed samples by high-performance liquid chromatography with mass spectrometry (HPLC-MS/MS) to determine the concentrations of four phthalate metabolites: mono (2-ethylhexyl) phthalate (MEHP), monobutyl phthalate (MnBP), monoethyl phthalate (MEP), and monoisononyl phthalate (MiNP). We observed significantly higher urinary concentrations of all monitored phthalate metabolites collected during the summer in occupationally exposed group (MEP p < 0.0015, MiNP p < 0.0001, MnBP p < 0.00019, and MEHP p < 0.05); however, in general, population was noticed this difference only for MEHP (p < 0.05) in winter season. We conclude that increasing indoor and outdoor temperature is related to phthalate exposure in specific types of work environment.

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  • Bornehag CG, Lundgren B, Wechler CJ, Sigsgaard T, Hagerhed-Engman L, Sundell J (2005) Phthalates in indoor dust and their association with building characteristics. Environ Health Perspect 113:1399–1404

    Article  CAS  Google Scholar 

  • Caserta D, Mantovani A, Marci R, Fazi A, Ciardo F, La Rocca C, Maranghi F, Moscarini M (2011) Environment and women’s reproductive health. Hum Reprod Update 17(3):418–433

    Article  CAS  Google Scholar 

  • CDC (2005) Third National Report on Human Exposure to Environmental Chemicals. ( Atlanta, GA, Centers for Disease Control and Prevention; National Center for Environmental Health; Division of Laboratory Sciences. 11-4-2007

  • Clausen PA, Liu Z, Kofoed-Sorensen V, Wolkoff P (2012) Influence of temperature on the emission of di-(2-ethylhexyl) phthalate (DEHP) from PVC flooring in the emission cell FLEC. Environ Sci Technol 46:909–915

    Article  CAS  Google Scholar 

  • Duty SM, Calafat AM, Silva MJ, Ryan L (2005) Phthalate exposure and reproductive hormones in adult men. Hum Reprod 20:604–610

    Article  CAS  Google Scholar 

  • Fong JP, Lee FJ, Lu IS, Uang SN, Lee CC (2014) Estimating the contribution of inhalation exposure to di-2-ethylhexyl phthalate (DEHP) for PVC production workers, using personal air sampling and urinary metabolite monitoring. Int J Hyg Environ Health 217(1):102–129

    Article  CAS  Google Scholar 

  • Fujii M, Shinohara N, Lim A, Otake T, Kumagai K, Yanagisawa Y (2003) A study on emission of phthalate esters from plastic materials using a passive flux sampler. Atmos Environ 37:5495–5504

    Article  CAS  Google Scholar 

  • Gaudin R, Marsan P, Ndaw S, Robert A, Ducos P (2011) Biological monitoring of exposure to di(2-ethylhexyl) phthalate in six French factories: a field study. Int Arch Occup Environ Health 84:523–531

    Article  CAS  Google Scholar 

  • Guo Y, Wu Q, Kannan K (2011) Phthalate metabolites in urine from China, and implications for human exposures. Environ Int 37(5):893–8

    Article  CAS  Google Scholar 

  • Hauser R, Calafat AM (2005) Phthalates and human health. Occup Environ Med 62:806–818

    Article  CAS  Google Scholar 

  • Heudorf M, Mersch-Sundermann V, Angerer J (2007) Phthalates: Toxicology and exposure. Int J Hyg Environ Health 210:623–634

    Article  CAS  Google Scholar 

  • Hines CJ, Nilsen Hopf NB, Deddens JA, Calafat AM, Silva MJ, Grote AA, Sammons DL (2009) Urinary phthalate metabolite concentrations among workers in selected industries: a pilot biomonitoring study. Ann Occup Hyg 53(1):1–17

    Article  CAS  Google Scholar 

  • Jaakkola JJK, Knight TL (2008) The role of exposure to phthalates from polyvinyl chloride products in the development of asthma and allergies. Environ Health Perspect 116:845–853

    Article  CAS  Google Scholar 

  • Jonsson BA, Richtoff J, Rylander L, Giwercman A, Haqmar L (2005) Urinary phthalate metabolites and biomarkers of reproductive function in young men. Epidemiology 16(4):487–493

    Article  Google Scholar 

  • Kavlock R, Boeckelheide K, Chapin R, Cunningham M, Faustman E, Foster P, Golub M, Henderson R, Hinberg I, Little R, Seed J, Shea K, Tabacova S, Tyl R, Williams P, Zacharewski T (2002) NTP Center for the Evaluation of Risks to Human Reproduction: Phthalates expert panel report on the reproductive and developmental toxicity of di(2-ethylhexyl)phthalate. Reprod Toxicol 16:529–653

    Article  CAS  Google Scholar 

  • Koch HM, Calafat AM (2009) Human body burdens of chemicals used in plastic manufacture. Philos Trans R Soc Lond B Biol Sci 364:2063–2078

    Article  CAS  Google Scholar 

  • Koch HM, Rossbach B, Drexler H, Angerer J (2003) Internal exposure of the general population to DEHP and other phthalates—Determination of secondary and primary phthalate monoester metabolites in urine. Environ Res 93:177–185

    Article  CAS  Google Scholar 

  • Kolarik B, Naydenov K, Larsson M, Bornehag CG, Sundell J (2008) The association between phthalates in dust and allergic diseases among Bulgarian children. Environ Health Perspect 116:98–103

    Article  CAS  Google Scholar 

  • Koniecki D, Wang R, Moody RP, Zhu J (2011) Phthalates in cosmetic and personal care products: Concentrations and possible dermal exposure. Environ Res 111(3):329–336

    Article  CAS  Google Scholar 

  • Silva M, Slakman AR, Reidy JA, Preau JR, Herbert JL, Samandar E, Needham LL, Calafat AM (2004a) Analysis of human urine for fifteen phthalate metabolites using automated solid-phase extraction. J Chromatogr B 805(2004):161–167

    Article  CAS  Google Scholar 

  • Silva MJ, Barr DB, Reidy JA, Malek NA (2004b) Urinary levels of seven phthalate metabolites in the US population from the National Health and Nutrition Examination Survey (NHANES) 1999–2000. Environ Health Perspect 112:331–338

    Article  CAS  Google Scholar 

  • Stanley MK, Robillard KA, Staples CHA (2003) Introduction. Handbook of environmental chemistry 3Q, s. 1–7

  • Swan SH (2008) Enviromental phthalate exposure in relation to reproductive outcomes and another health endpoints in humans. In: Environ res. 105:177–184

  • Wittassek M, Koch HM, Angerer J, Bruning T (2011) Assessing exposure to phthalates-The human biomonitoring approach. Mol Nutr Food Res 55:7–31

    Article  CAS  Google Scholar 

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This study was supported by projects VEGA “Analysis of selected environmental factors in relation to potential health risks” (V1/0042/12) and the project implementation: “Environmental aspects of urban area” (ITMS: 26220220110) supported by the Research & Development Operational Programme funded by the ERDF. We thank Michaela Földesiova for excellent technical assistance.

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The authors declare that they have no conflict of interest.

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Correspondence to Tomas Pilka.

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Responsible editor: Hongwen Sun

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Pilka, T., Petrovicova, I., Kolena, B. et al. Relationship between variation of seasonal temperature and extent of occupational exposure to phthalates. Environ Sci Pollut Res 22, 434–440 (2015).

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