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Experimental and kinetic study on the cometabolic biodegradation of phenol and 4-chlorophenol by psychrotrophic Pseudomonas putida LY1

Environmental Science and Pollution Research volume 22, pages 565–573 (2015)Cite this article

Abstract

This study investigated the kinetics of phenol and 4-chlorophenol (4-CP) biodegradation by a cold-adapted bacteria, Pseudomonas putida LY1, isolated from Songhua River sediment. The results showed that P. putida LY1 cannot grow on 4-CP as a sole carbon source. P. putida LY1 had the potential to cometabolic biodegrade phenol and 4-CP in a wide range of temperature (varying from 5 to 35 °C) with the optimal temperature around 25 °C. Mixture of phenol and 4-CP were completely removed at two 4-CP concentrations (15 and 40 mg/L) over a wide range of phenol (20–400 mg/L) concentrations, whereby the ratio of 4-CP/biomass (S 2/X) was lower than 0.03. The kinetic models of cometabolic biodegradation of phenol and 4-CP were proposed, considering the growth and nongrowth substrate inhibition. These models successfully simulate the processes of cometabolic degradation of phenol and 4-CP.

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Acknowledgments

The authors greatly appreciate financial support from the National Natural Science Foundation of China (No. 51322901), Jiangsu Province Ordinary University Graduate Student Scientific Research Innovation Plan (No. CXZZ12_0258), and the Fundamental Research Funds for the Central Universities (No. 2013B05214).

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Affiliations

  1. Key Laboratory of Integrated Regulation and Resource Development of Shallow Lakes, Ministry of Education, College of Environment, Hohai University, Xikang Road #1, Nanjing, 210098, People’s Republic of China

    Qing Wang, Yi Li, Jing Li, Yuming Wang, Chao Wang & Peifang Wang

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  1. Qing Wang
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  2. Yi Li
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  3. Jing Li
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  4. Yuming Wang
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  5. Chao Wang
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  6. Peifang Wang
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Correspondence to Yi Li.

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Responsible editor: Robert Duran

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Wang, Q., Li, Y., Li, J. et al. Experimental and kinetic study on the cometabolic biodegradation of phenol and 4-chlorophenol by psychrotrophic Pseudomonas putida LY1. Environ Sci Pollut Res 22, 565–573 (2015). https://doi.org/10.1007/s11356-014-3374-x

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  • DOI: https://doi.org/10.1007/s11356-014-3374-x

Keywords

  • Cometabolic biodegradation
  • Phenol
  • 4-Chlorophenol
  • Pseudomonas putida LY1
  • Kinetics