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Determination of phthalates and their by-products in tissues of roach (Rutilus rutilus) from the Orge river (France)

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Abstract

Seven phthalate by-products were investigated for the first time, in target tissues of roach from a contaminated river of the Ile-de-France district. All parent phthalates were bioaccumulated in liver and muscle and liver contents were correlated with river concentrations (p < 0.01). All metabolites were found in liver, plasma and bile. The mono-iso-butyl phthalate (MiBP; 1.6 μg g−1 dw of liver), followed by mono-n-butyl phthalate (MnBP; 1.5 μg g−1 dw of liver) were the most abundant ones. Among the three metabolites of di-ethylhexyl phthalate (DEHP), mono (2-ethylhexyl) phthalate (MEHP) predominated in bile (15.5 ng ml−1) and liver (0.237 μg g−1 dw), whereas in plasma, it was mono (2-ethyl-5-hydroxyhexyl) phthalate – MEHHP (214 ng ml−1). In liver, MEHP/DEHP ratios ranged from 0.04 to 0.2. Among the oxidized metabolites, only mono (2-ethyl-5-oxohexyl) phthalate (MEOHP) was correlated (p < 0.05) with parent DEHP and appeared to be a more reliable marker of DEHP impact than the monoester.

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Acknowledgments

This work was supported by the PIREN-SEINE Research Programme. The French National Agency for Water and Aquatic Environments (ONEMA) provided assistance for electric fishing. We thank Catherine Bourges and Annie Desportes for their technical assistance.

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Authors and Affiliations

  1. Université Paris VI/CNRS-UMR 7619 METIS, Tour 56-46, case courrier 105, 4 place Jussieu, 75252, Pari, Cedex 05s, France

    A. S. Valton, C. Serre-Dargnat, M. Blanchard, F. Alliot, M. Chevreuil & Marie Jeanne Teil

  2. Laboratoire «Hydrologie et Environnement», Ecole Pratique des Hautes Etudes, UMR 7619 Sisyphe, Université Paris VI, Tour 56-46, case courrier 105, 4 place Jussieu, 75252, Paris, Cedex 05, France

    M. Blanchard, F. Alliot, M. Chevreuil & Marie Jeanne Teil

Authors
  1. A. S. Valton
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  2. C. Serre-Dargnat
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  3. M. Blanchard
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  4. F. Alliot
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  5. M. Chevreuil
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  6. Marie Jeanne Teil
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Correspondence to Marie Jeanne Teil.

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Responsible editor: Roland Kallenborn

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Valton, A.S., Serre-Dargnat, C., Blanchard, M. et al. Determination of phthalates and their by-products in tissues of roach (Rutilus rutilus) from the Orge river (France). Environ Sci Pollut Res 21, 12723–12730 (2014). https://doi.org/10.1007/s11356-014-3213-0

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  • DOI: https://doi.org/10.1007/s11356-014-3213-0

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