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Environmental Science and Pollution Research

, Volume 21, Issue 16, pp 9589–9603 | Cite as

Faecal pollution loads in the wastewater effluents and receiving water bodies: a potential threat to the health of Sedibeng and Soshanguve communities, South Africa

  • Giorgis Z. Teklehaimanot
  • Martie A. A. Coetzee
  • Maggy N. B. Momba
Research Article

Abstract

The discharge of untreated or inadequately treated effluents has been identified among the activities responsible for the spread of a wide range of potentially infectious agents. The aim of this study was to determine whether inadequate treatment of wastewater and the faecal pollution load of effluents and receiving water bodies in Sedibeng District and Soshanguve peri-urban area of the Tshwane Metropolitan Municipality could be a potential threat to the health of the surrounding communities. Variations in the counts of faecal indicator bacteria and pathogenic microorganisms and compliance of the effluents and receiving water bodies with South African and World Health Organization standards were assessed between August 2011 and May 2012 using culture-based methods and molecular techniques. The overall quality of effluents did not comply with the South African special standard of no risk for unrestricted irrigation (zero Escherichia coli/100 ml). The quality of the receiving water bodies did not comply with South African regulatory limits set for domestic purposes (zero E. coli/100 ml, <30 faecal enterococci/100 ml and <1 somatic coliphages/100 ml), for full contact recreation (<20 somatic coliphages/100 ml) and aquaculture (<10 E. coli/100 ml) and WHO standards for full and intermediate contact recreational use (<1 E. coli/100 ml and <40 faecal enterococci/100 ml, respectively). The PCR results revealed the prevalence of pathogenic microorganisms; between 0 and 60 % of samples tested positive for Salmonella Typhimurium and Shigella dysenteriae, and between 20 and 60 % of samples tested positive for Vibrio cholerae. These findings demonstrated that potential health risks might be associated with the use of the target river waters for domestic, recreational and irrigation purposes. This study calls for a prompt intervention to improve wastewater management.

Keywords

Faecal pollution Water quality Wastewater effluent Pollution indicators Pathogenic bacteria Health risk 

Notes

Acknowledgments

The authors would like to thank the National Research Foundation (NRF) and the Tshwane University of Technology for the funding of this project.

References

  1. Adewumi JR, Ilemobade AA, Van Zyl JE (2010) Treated wastewater reuse in South Africa: Overview, potential and challenges. Resour Conserv Recycl 55:221–231CrossRefGoogle Scholar
  2. APHA (2001) Revisions to standard methods for the examination of water and wastewater (supplement). American Public Health Association, Washington DC, USAGoogle Scholar
  3. Bisson JW, Cabelli VJ (1979) Membrane filter enumeration method for Clostridium perfringens. Appl Environ Microbiol 37(1):55–66Google Scholar
  4. Bisson JW, Cabelli VJ (1980) Clostridium perfringens as a water pollution indicator. J Water Pollut Control Fed 52(2):241–248Google Scholar
  5. Brözel VS, Cloete TE (1991) Fingerprinting of commercially available water treatment bactericides in South Africa. Water SA 17(1):57–66Google Scholar
  6. Burkhardt W III, Calci KR, Watkins WD, Rippey SR, Chirtel SJ (2000) Inactivation of indicator microorganisms in estuarine waters. Water Res 34:2207–2214CrossRefGoogle Scholar
  7. Castillo MM, Allan JD, Sinsabaugh RL, Kling GW (2004) Seasonal and inter-annual variation of bacterial production in lowland rivers of the Orinoco basin. Freshw Biol 49:1400–1414CrossRefGoogle Scholar
  8. Cimenti M, Hubberstey A, Bewtra JK, Biswas N (2007) Alternative methods in tracking sources of microbial contamination in waters. Water SA 33:183–193Google Scholar
  9. Das A, Mazumder Y, Dutta BR, Shome BR, Bujarbaruah KM, Kumar R (2012) Molecular typing of Clostridium perfringens isolated from diarrhoeic cattle. J Anim Sci Adv 2(2):226–229Google Scholar
  10. Department of Health (2006) Mortality and morbidity among women and children. Annual Report, Government Printer, Pretoria South AfricaGoogle Scholar
  11. Dickens CWS, Graham PM (1998) Biomonitoring for effective management of wastewater discharges and the health of the river environment. Aquat Ecosyst Health Manag 1:199–217Google Scholar
  12. DWA (2011) South African Waste Water Quality Management Performance. Green Drop Report. Green Drop Certification. Department of Water Affairs and Forestry, Pretoria, South AfricaGoogle Scholar
  13. DWA (2012) South African Waste Water Quality Management Performance. Green Drop Report. Green Drop Certification. Department of Water Affairs and Forestry, Pretoria, South AfricaGoogle Scholar
  14. DWAF (1996a) South African Water Quality Guidelines (2nd edn.) Vol. 2: Domestic Use. Department of Water Affairs and Forestry, Pretoria, South Africa, pp 86–87Google Scholar
  15. DWAF (1996b) Community water supply and sanitation: Strategic Study-National Assessment. Department of Water Affairs and Forestry. South Africa, PretoriaGoogle Scholar
  16. DWAF (2004) Revision of General Authorizations in terms of Section 39 of the National Water Act, 1998 (Act No. 36 of 1998). South African Government Gazette No. 26187. Department of Water Affairs and Forestry, Pretoria, South AfricaGoogle Scholar
  17. DWAF (2009) Water Resources Planning Systems. Orange River: assessment of water quality data requirements for water quality planning purposes. Report No.5. PRSA D000/00/8009/2. Department of Water Affairs and Forestry, Pretoria, South AfricaGoogle Scholar
  18. Edberg SC, Rice EW, Karlin RJ, Allen MJ (2000) Escherichia coli: the best biological drinking water indicator for public health protection. J Appl Microbiol 88:106–116CrossRefGoogle Scholar
  19. Enriquez C, Nwachuku N, Gerba CP (2001) Direct exposure to animal enteric pathogens. Rev Environ Health 16(2):117–131CrossRefGoogle Scholar
  20. Espinosa AC, Arias CF, Sánchez-Colón S, Mazari-Hiriart M (2009) Comparative study of enteric viruses, coliphages and indicator bacteria for evaluating water quality in a tropical high-altitude system. Environ Health 8:49CrossRefGoogle Scholar
  21. Field KG, Samadpour M (2007) Faecal sources tracking, the indicator paradigm and managing water quality. Water Res 41(16):3517–3538CrossRefGoogle Scholar
  22. Gerba CP, Rose JB (1990) Viruses in sources and drinking water. In: McFeters GA (ed) Drinking Water Microbiology. Springer-Verlag, New York, pp 380–396CrossRefGoogle Scholar
  23. Gleeson C, Gray N (1997) The coliform index and waterborne diseases: Problems of microbial drinking water assessment. E & F.N. Spon Ltd., London, UKGoogle Scholar
  24. Goel AK, Ponmariappan S, Kamboj DV and Singh L (2007) Single multiplex polymerase chain reaction for environmental surveillance for toxigenic-pathogenic O1 and non-O1 Vibrio cholerae. Folia Microbiological 52(1):81–85Google Scholar
  25. Grabow WOK (2001) Bacteriophages: Update on application as models for viruses in water. Water SA 27:251–268Google Scholar
  26. Haigh EH, Fox HE, Davies-Coleman HD (2010) Framework for local governments to implement integrated water resources management linked to water services delivery. Water SA 36(4):475–486CrossRefGoogle Scholar
  27. Igbinosa EO, Okoh AI (2009) Impact of discharge wastewater effluents on the physicochemical qualities of a receiving water shed in a typical rural community. Int J Environ Sci Technol 6:175–182CrossRefGoogle Scholar
  28. ISO (2000) Water quality—detection and enumeration of bacteriophages: enumeration of somatic coliphages. International Standards Organization, ISO 10705, Part 2Google Scholar
  29. Jessup D (2001) Marine mammals face increased mortality rate. The Environmental and Energy Study Institute, Washington DCGoogle Scholar
  30. Jofre J, Olle E, Ribas F, Vidal A, Lucena F (1995) Potential usefulness of bacteriophages that infect Bacteriodes fragilis as model organisms for monitoring virus removal in drinking water treatment plants. Appl Environ Microbiol 61(9):3227–3231Google Scholar
  31. Ke D, Picard FJ, Martineau F, Menard C, Roy PH, Ouellette M, Bergeron MG (1999) Development of a PCR assay for rapid detection of enterococci. J Clin Microbiol 37:3497–3503Google Scholar
  32. Kong RYC, Lee SKY, Law TWF, Law SHW, Wu RSS (2002) Rapid detection of six types of bacterial pathogens in marine waters by multiplex PCR. Water Res 36:2802–2812CrossRefGoogle Scholar
  33. Lederberg J (1999) Encyclopedia of microbiology. Academic Press, San Diego CA, p 2000Google Scholar
  34. Maddocks S, Olma T, Chen S (2002) Comparison of CHROMagar Salmonella medium and Xylose-Lysine-Desoxycholate and Salmonella-Shigella agars for isolation of Salmonella strains from stool samples. J Clin Microbiol 40(8):2999–3003CrossRefGoogle Scholar
  35. Maimon A, Tal A, Friedler E, Gross A (2010) Safe on-site reuse of greywater for irrigation—a critical review of current guidelines. Environ Sci Technol 44:3213–3220CrossRefGoogle Scholar
  36. Mara D (1976) Sewage treatment in hot climates. John Wiley, New York, USAGoogle Scholar
  37. McFeters GA, Bissonnette GK, Jezeski JJ (1974) Comparative survival of indicator bacteria and enteric pathogens in well water. Appl Environ Microbiol 27:823–829Google Scholar
  38. McQuaig SM, Scott TM, Harwood VJ, Farrah SR, Lukasik JO (2006) Detection of human derived faecal pollution in environmental waters by use of a PCR-based human polyomavirus assay. Appl Environ Microbiol 72(12):7567–7574CrossRefGoogle Scholar
  39. Merck Catalogue Number 1.07667.0500. (2014) Salmonella-Shigella Agar. Salmonella and Shigella diagnostic Agar. Merck KGaA, Darmstadt, GermanyGoogle Scholar
  40. Merck Catalogue Number 1.10263.0500. (2014) Thiosulfate Citrate Bile Salt Agar. Vibrio diagnostic Agar. Merck KGaA, Darmstadt, GermanyGoogle Scholar
  41. Metcalf and Eddy (2004). Wastewater engineering: treatment and reuse (4th ed.). Revised by: Techobanoglous, G., Burton, FL. & Stensel, HD. McGraw-Hill, New YorkGoogle Scholar
  42. Mocé-Llivina L, Muniesa M, Pimenta-Vale H, Lucena F, Jofre J (2003) Survival of bacterial indicator species and bacteriophages after thermal treatment of sludge and sewage. Appl Environ Microbiol 69(3):1452–1456CrossRefGoogle Scholar
  43. Momba MNB and Mfenyana C (2005) Inadequate treatment of wastewater: a source of coliform bacteria in receiving surface water bodies in developing countries—case study: Eastern Cape Province of South Africa. In: Lehr JH and Keeley J (eds.) Water Encyclopaedia - Domestic, Municipal and Industrial Water Supply and Waste Disposal. John Wiley & Sons. pp. 661-667Google Scholar
  44. Momba MNB, Osode AN, Sibewu M (2006) The impact of inadequate wastewater treatment on the receiving water bodies-case study: Buffalo City and Nkonkobe Municipalities of the Eastern Cape. Water SA 32(5):687–692Google Scholar
  45. Nandi B, Nandy RK, Mukhopadhyay S, Nair GB, Shimada T, Ghose AC (2000) Rapid method for species-specific identification of Vibrio using primers targeted to the gene of outer membrane protein OmpW. J Clin Microbiol 38(11):4145–4151Google Scholar
  46. Noble RT, Blackwood AD, Griffith JF, McGee CD, Weisberg SB (2010) Comparison of rapid quantitative PCR-based and conventional culture-based methods for enumeration of Enterococcus spp. and Escherichia coli in recreational waters. Appl Environ Microbiol 76(22):7437–7443CrossRefGoogle Scholar
  47. Payment P, Franco E (1993) Clostridium perfringens and somatic coliphages as indicators of the efficiency of drinking water treatment for viruses and protozoan cysts. Appl Environ Microbiol 59(8):2418–2424Google Scholar
  48. Peter D, Andrew AC, Alex DH (2000) Emerging infectious diseases of wildlife: threat to biodiversity and human health. Sci 287:443–445CrossRefGoogle Scholar
  49. Postel SL, Daily GC, Ehrlich PR (1996) Human appropriation of renewable fresh water. Sci 271(5250):785–788CrossRefGoogle Scholar
  50. SABS (2003) Water quality—detection and enumeration of Vibrio cholerae. South African National Standards (edn. 1). SANS Method 6315. South African Bureau of Standards, Pretoria, South Africa, pp 1–14Google Scholar
  51. Samie A, Obi CL, Igumbor JO, Momba MNB (2009) Focus on 14 sewage treatment plants in the Mpumalanga Province, South Africa in order to gauge the efficiency of wastewater treatment. Afr J Biotechnol 8(14):3276–3285Google Scholar
  52. Savichtcheva O, Okabe S (2006) Alternative indicators of faecal pollution: Relations with pathogens and conventional indicators, current methodologies for direct pathogen monitoring and future application perspectives. Water Res 40:2463–2476CrossRefGoogle Scholar
  53. Simpson JM, Santo Domingo JW, Reasoner DJ (2002) Microbial source tracking: state of the science. Environ Sci Technol 36(24):5279–5288CrossRefGoogle Scholar
  54. Slots J (1986) Bacterial specificity in adult periodontitis. A summary of recent work. I. Clin Periodontol 13:912–917CrossRefGoogle Scholar
  55. Snyman HG, Van Niekerk AM and Rajasakran N (2006) Sustainable wastewater treatment—What has gone wrong and how do we get back on track. Proceedings of the Water Institute of South Africa (WISA) Conference, Durban, 21-25 May 2006Google Scholar
  56. StataCorp (2009) Stata time-series reference manual. Release 11, Statistical Software, College station, TX: StataCorp LP, Texas, USAGoogle Scholar
  57. Stoeckel DM, Harwood VJ (2007) Performance, design and analysis in microbial sources tracking studies. Appl Environ Microbiol 73(8):2405–2415CrossRefGoogle Scholar
  58. Stroeher UH, Karageorgos LE, Morona R, Manning PA (1992) Serotype conversion in Vibrio cholerae 01. Proc Natl Acad Sci U S A 89(7):2566–2570CrossRefGoogle Scholar
  59. Sundram A, Donnelly L, Ehlers MM, Vrey A, Grabow WOK, Bailey IW (2002) Evaluation of FENA as indicators of viruses and sources of faecal pollution. Water SA. Special edition WISA proceedings. Available from: http://www.wrc.org.za/Knowledge%20Documents/Water%20SA%20Journals/Manuscripts/2002/05/WaterSA_2002_05_18.pdf. Accessed 07 March 2014
  60. Thiem VD, Sethabutr O, Seidlein LV, Tung TV, Canh DG, Chien BT, Tho LH, Lee H, Houng HS, Hale TL, Clemens JD, Mason C, Trach DD (2004) Detection of Shigella by PCR assay targeting the ipaH gene suggests increased prevalence of Shigellosis in Nha Trang. Vietnam J Clin Microbiol 42:2031–2035CrossRefGoogle Scholar
  61. Tiefenthaler L, Stein ED, Schiff KC (2011) Levels and patterns of faecal indicator bacteria in stormwater runoff from homogenous land use sites and urban watersheds. J Water Health 9:279–290CrossRefGoogle Scholar
  62. Tsai YL, Palmer CJ, Sangermano LR (1993) Detection of Escherichia coli in sewage and sludge by polymerase chain reaction. Appl Environ Microbiol 59:353–357Google Scholar
  63. UNESCO/WHO/UNEP (1996) Water quality assessment: a guide to use of biota, sediments and water environment (2nd edn.). Cambridge University Press, CambridgeGoogle Scholar
  64. USEPA (2001) EPA Method 1602: male specific (F+) and somatic coliphages enumeration in water by single layer (SAL) procedure. EPA Report 821-R-01-029. US Environmental Protection Agency, Washington DC, USAGoogle Scholar
  65. WHO (1996) Guidelines for drinking water quality (2nd edn.). Vol. 2. World Health Organization, Geneva, SwitzerlandGoogle Scholar
  66. WHO (2001) Guidelines: the current position. In: Lorna F, Jamie B (eds) Water quality: Guidelines, Standards and Health. IWA Publishing, London, UKGoogle Scholar
  67. WHO (2004) Guidelines for drinking-water quality (3rd edn.). Vol. 1: Recommendation. World Health Organization, Geneva, Switzerland, pp 259–274Google Scholar
  68. WHO (2009a) Cholera annual report 2008. Wkly Epidemiol Rec 84(31):309–324Google Scholar
  69. WHO (2009b) Global task force on cholera control. World Health Organization. Available from: www.who.int/cholera/countries/Zimbabwecountry profile2009.pdf. Accessed on 07 October 2012
  70. Wilkes G, Edge T, Gannon V, Jokiene C, Lyautey E, Medeiros D, Neumann N, Ruecker N, Topp E, Lapen DR (2009) Seasonal relationships among indicator bacteria, pathogenic bacteria, Cryptosporidium oocysts, Giardia cysts and hydrogeological indices for surface waters within an agricultural landscape. Water Res 43(8):2209–2223CrossRefGoogle Scholar
  71. Wohlsen T, Bayliss J, Gray B, Bates J, Katouli M (2006) Evaluation of an alternative method for the enumeration and confirmation of Clostridium perfringens from treated and untreated sewage. Lett Appl Microbiol 42:438–444CrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 2014

Authors and Affiliations

  • Giorgis Z. Teklehaimanot
    • 1
  • Martie A. A. Coetzee
    • 1
  • Maggy N. B. Momba
    • 1
  1. 1.Water Care Unit, Department of Environmental, Water and Earth SciencesTUTPretoriaSouth Africa

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