Abstract
Two petroleum-degrading strains were screened from oil fields and denoted as SWH-1 (Bacillus subtilis) and SWH-2 (Sphingobacterium multivorum), which were used to ferment and prepare bacterial agent to remediate petroleum-contaminated sites in Shengli Oil Field in China. The optimal liquid fermentation medium and conditions were MgSO4·7H2O (0.5 %), NaCl (0.5 %), soybean dregs (3 %), pH 7.0, culturing at 30 °C, and 220 r/min for 16 h. Peat was chosen as the bacterial carrier due to its ability of keeping microbial activity. Mixed fermented liquid was added into peat (1:2) and air-dried, and the bacterial agent was obtained. It was applied to the petroleum-contaminated soil, which was irrigated, tilled, and fertilized. The removal rate reached 67.7 % after 2 months of remediation. During remediation, the quantity of indigenous bacteria varied a lot, while the inoculated bacteria remained stable; the dehydrogenase activity was at high levels and then decreased. Indigenous microorganisms, inoculated bacterial agent, nutrients, water, and soil permeability all played important roles. The study prepared an environment-friendly bacterial agent and established a set of bioremediation technique, which provided further insights into integration of fermentation engineering and soil remediation engineering.
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Acknowledgments
This work was funded by the International S&T Cooperation Program of China (2013DFR90540), National High Technology Research and Development Program (“863” Program) of China (2013AA06A210), Natural Science Foundation of Shandong Province (ZR2011DQ002), Science and Technology Development Program of Shandong Province (2013GSF11703), Environmental Bottlenecks Analysis and Breakthrough Program of Shandong Province (SDHBPJ-ZB-07), the Youth Fund of Shandong Academy of Sciences (2013QN015), and Open Program of MOE Key Laboratory of Pollution Processes and Environmental Criteria (KL-PPEC-2013-12).
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Yu, Y., Zhang, W., Chen, G. et al. Preparation of petroleum-degrading bacterial agent and its application in remediation of contaminated soil in Shengli Oil Field, China. Environ Sci Pollut Res 21, 7929–7937 (2014). https://doi.org/10.1007/s11356-014-2707-0
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DOI: https://doi.org/10.1007/s11356-014-2707-0